Photoacoustic monitoring of neovascularities in grafted skin

In skin grafting, evaluation of graft adhesion to the recipient site in the early postgrafting period is important. However, conventional diagnoses such as visual observation and thermography required about 1 week to obtain results and these methods cannot give quantitative information on the adhesion of a skin graft. We proposed a new method for monitoring adhesion of grafted skin that is based on measurement of photoacoustic signals. To investigate the validity of the method, we performed experiments using rat autografts models.

[1]  Setsuo Takatani,et al.  Theoretical Analysis of Diffuse Reflectance from a Two-Layer Tissue Model , 1979, IEEE Transactions on Biomedical Engineering.

[2]  Masanori Fujita,et al.  Photoacoustic signal measurement for burned skins in the spectral range of 500-650 nm: experiment with rat burn models , 2002, SPIE BiOS.

[3]  V. Tron,et al.  Comparison of Donor‐Site Healing under Xeroform and Jelonet Dressings: Unexpected Findings , 2003, Plastic and reconstructive surgery.

[4]  Roy G. M. Kolkman,et al.  In vivo photoacoustic imaging of blood vessels using an extreme-narrow aperture sensor , 2003 .

[5]  Bernard Choi,et al.  In vivo port-wine stain depth determination with a photoacoustic probe. , 2003, Applied optics.

[6]  F. Dunn,et al.  Comprehensive compilation of empirical ultrasonic properties of mammalian tissues. , 1978, The Journal of the Acoustical Society of America.

[7]  Tsunenori Arai,et al.  Measurement of photoacoustic signals from skin: potential application to burn depth estimation , 2001, European Conference on Biomedical Optics.

[8]  P. Carmeliet.  Mechanisms of angiogenesis and arteriogenesis , 2000, Nature Medicine.

[9]  A. Mason,et al.  Enhanced survival of autoepidermal-allodermal composite grafts in allosensitized animals by use of silver-nylon dressings and direct current. , 1995, The Journal of trauma.

[10]  Alexander A. Oraevsky,et al.  Laser optoacoustic imaging of breast cancer in vivo , 2001, SPIE BiOS.

[11]  Rakesh K. Jain,et al.  Quantitative angiogenesis assays: Progress and problems , 1997, Nature Medicine.

[12]  Steven L. Jacques,et al.  Depth profiling of absorbing soft materials using photoacoustic methods , 1999 .

[13]  Robert A Kruger,et al.  Thermoacoustic molecular imaging of small animals. , 2003, Molecular imaging.

[14]  Martin Frenz,et al.  Comparision of laser-induced and classical ultasound , 2003, SPIE BiOS.

[15]  Shunichi Sato,et al.  Adhesion monitoring of skin grafts by photoacoustic measurement: experiment using rat allograft models , 2004, SPIE BiOS.

[16]  P. Branemark,et al.  The vascularization of a free full thickness skin graft. 3. An infrared thermographic study. , 1969, Scandinavian journal of plastic and reconstructive surgery.

[17]  Cherry,et al.  Dermal oedema assessed by high frequency ultrasound in venous leg ulcers , 1998, The British journal of dermatology.

[18]  Lihong V. Wang,et al.  In vivo dark-field reflection-mode photoacoustic microscopy. , 2005, Optics letters.

[19]  Miya Ishihara,et al.  Photoacoustic detection of neovascularities in skin graft , 2005, SPIE BiOS.

[20]  Geng Ku,et al.  Three-dimensional laser-induced photoacoustic tomography of mouse brain with the skin and skull intact. , 2003, Optics letters.