DETECTION OF SPECIES IN MEAT AND MEAT PRODUCTS USING ENZYME‐LINKED IMMUNOSORBENT ASSAY

Detection of species adulteration in meat products is important for consumer protection and food labeling law enforcement. In this study, samples of 28 fermented sausages; 14 cooked salami; 11 frankfurters; 9 raw meats; 16 raw ground meats and meat balls; 3 pastramis, 2 hams and 5 bacons; 7 cooked meats; and 5 canned products resulting in a total of 100 meat and meat products were analyzed for species determination by enzyme-linked immunosorbent assay test kits prepared with monoclonal antibody technique. Results showed that 11 of 28 fermented sausages (39.2%), 5 of 14 cooked salami (35.7%), 3 of 11 frankfurters (27.2%), 2 of 9 raw meat (22.2%) and 1 of 16 raw ground meat and meat ball (6.2%) samples were found to contain undeclared species. Fermented sausage, cooked salami and frankfurter samples that had been declared as beef only contained poultry meat. Raw meat samples that were declared as beef were determined as horse and deer meat. One meatball sample declared as beef was found to be poultry meat. These results indicate that 22.0% of the samples were not in compliance with Turkish Food Codex violating consumer rights and presenting a potential public health risk. A broad national control program by central authority is required to protect consumers and to prevent unfair competition.

[1]  M. Saito,et al.  Polymerase chain reaction assay for detection of sheep and goat meats , 1994 .

[2]  P S Girish,et al.  Sequence analysis of mitochondrial 12S rRNA gene can identify meat species. , 2004, Meat science.

[3]  B. Tremlova Histologischer Nachweis von Knochenpartikeln in Fleischprodukten , 2000 .

[4]  V. S. Reddy,et al.  Identification of species origin of fresh, cooked and decomposed meats using brain antigens. , 2000 .

[5]  R. Johnston,et al.  Detection of poultry and pork in cooked and canned meat foods by enzyme-linked immunosorbent assays. , 1988, Journal - Association of Official Analytical Chemists.

[6]  Y. Hsieh,et al.  Detection of Species Substitution in Raw and Cooked Meats Using Immunoassays. , 1995, Journal of food protection.

[7]  C. Milstein,et al.  Continuous cultures of fused cells secreting antibody of predefined specificity , 1975, Nature.

[8]  R. Patterson,et al.  Monoclonal antibody sandwich ELISA for the potential detection of chicken meat in mixtures of raw beef and pork. , 1991, Meat science.

[9]  Fur-Chi Chen,et al.  Monoclonal Antibodies to Porcine Thermal-Stable Muscle Protein for Detection of Pork in Raw and Cooked Meats , 2008 .

[10]  B. Jayarao,et al.  Studies on thermostable antigens, production of species-specific antiadrenal sera and comparison of immunological techniques in meat speciation. , 1993, Meat science.

[11]  E. Rencová,et al.  Identification by ELISA of poultry, horse, kangaroo, and rat muscle specific proteins in heat-processed products. , 2000 .

[12]  Y. Hsieh,et al.  DETECTION OF SPECIES ADULTERATION IN PORK PRODUCTS USING AGAR‐GEL IMMUNODIFFUSION AND ENZYME‐LINKED IMMUNOSORBENT ASSAY , 1996 .

[13]  K. Chikuni,et al.  Determination of mitochondrial cytochrome B gene sequence for red deer (Cervus elaphus) and the differentiation of closely related deer meats. , 1998, Meat science.

[14]  E. Rencová,et al.  Identification of bovine-specific DNA in feedstuffs. , 2001, Journal of food protection.

[15]  R. Johnston,et al.  Detection of Beef, Sheep, Deer, and Horse Meat in Cooked Meat Products by Enzyme-Linked Immunosorbent Assay , 1992 .

[16]  Z. Mingguang,et al.  Establishment and application of a polymerase chain reaction for the identification of beef. , 1999, Meat science.

[17]  R. Bevan,et al.  The use of a poultry-specific murine monoclonal antibody directed to the insoluble muscle protein desmin in meat speciation , 1996 .