Perception of UV-B by the Arabidopsis UVR8 Protein

A plant ultraviolet-B photoreceptor uses a tryptophan-based chromophore. To optimize their growth and survival, plants perceive and respond to ultraviolet-B (UV-B) radiation. However, neither the molecular identity of the UV-B photoreceptor nor the photoperception mechanism is known. Here we show that dimers of the UVR8 protein perceive UV-B, probably by a tryptophan-based mechanism. Absorption of UV-B induces instant monomerization of the photoreceptor and interaction with COP1, the central regulator of light signaling. Thereby this signaling cascade controlled by UVR8 mediates UV-B photomorphogenic responses securing plant acclimation and thus promotes survival in sunlight.

[1]  L. Björn,et al.  UV-B as an environmental factor in plant life: stress and regulation. , 1997, Trends in ecology & evolution.

[2]  R. D. Gietz,et al.  Transformation of yeast by lithium acetate/single-stranded carrier DNA/polyethylene glycol method. , 2002, Methods in enzymology.

[3]  Chih-Chieh Chen,et al.  (PS)2: protein structure prediction server , 2006, Nucleic Acids Res..

[4]  F. Nagy,et al.  Signalling and gene regulation in response to ultraviolet light. , 2005, Current opinion in plant biology.

[5]  Gareth I Jenkins,et al.  UV-B Promotes Rapid Nuclear Translocation of the Arabidopsis UV-B–Specific Signaling Component UVR8 and Activates Its Function in the Nucleus , 2007, The Plant Cell Online.

[6]  L. Klotz,et al.  Lightening up the UV response by identification of the arylhydrocarbon receptor as a cytoplasmatic target for ultraviolet B radiation , 2007, Proceedings of the National Academy of Sciences.

[7]  M. Wickens,et al.  Analysis of yeast prp20 mutations and functional complementation by the human homologue RCC1, a protein involved in the control of chromosome condensation , 1991, Molecular and General Genetics MGG.

[8]  Chentao Lin,et al.  Photoexcited CRY2 Interacts with CIB1 to Regulate Transcription and Floral Initiation in Arabidopsis , 2008, Science.

[9]  D. Kliebenstein,et al.  Arabidopsis UVR8 Regulates Ultraviolet-B Signal Transduction and Tolerance and Contains Sequence Similarity to Human Regulator of Chromatin Condensation 1 , 2002, Plant Physiology.

[10]  Pawel Herzyk,et al.  A UV-B-specific signaling component orchestrates plant UV protection. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[11]  H. Hirt,et al.  MAP KINASE PHOSPHATASE1 and PROTEIN TYROSINE PHOSPHATASE1 Are Repressors of Salicylic Acid Synthesis and SNC1-Mediated Responses in Arabidopsis[C][W] , 2009, The Plant Cell Online.

[12]  Y. Komeda,et al.  Genetic and molecular analysis of an allelic series of cop1 mutants suggests functional roles for the multiple protein domains. , 1994, The Plant cell.

[13]  A. Vojtek,et al.  Ras-Raf interaction: two-hybrid analysis. , 1995, Methods in enzymology.

[14]  H. Lehrach,et al.  A Human Protein-Protein Interaction Network: A Resource for Annotating the Proteome , 2005, Cell.

[15]  Geoffrey J. Barton,et al.  Jalview Version 2—a multiple sequence alignment editor and analysis workbench , 2009, Bioinform..

[16]  T. Kerppola,et al.  Visualization of molecular interactions by fluorescence complementation , 2006, Nature Reviews Molecular Cell Biology.

[17]  Gareth I Jenkins,et al.  UVR8 in Arabidopsis thaliana regulates multiple aspects of cellular differentiation during leaf development in response to ultraviolet B radiation. , 2009, The New phytologist.

[18]  Dorothee Staiger,et al.  Ultraviolet-B Radiation-Mediated Responses in Plants. Balancing Damage and Protection1 , 2003, Plant Physiology.

[19]  N. Paul,et al.  Ecological roles of solar UV radiation: towards an integrated approach , 2003 .

[20]  E. Huq,et al.  A light-switchable gene promoter system , 2002, Nature Biotechnology.

[21]  Alfred Wittinghofer,et al.  The 1.7 Å crystal structure of the regulator of chromosome condensation (RCC1) reveals a seven-bladed propeller , 1998, Nature.

[22]  Xing Wang Deng,et al.  COP1 - from plant photomorphogenesis to mammalian tumorigenesis. , 2005, Trends in cell biology.

[23]  E. Oakeley,et al.  Genome-wide analysis of gene expression reveals function of the bZIP transcription factor HY5 in the UV-B response of Arabidopsis. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[24]  Edward J Oakeley,et al.  CONSTITUTIVELY PHOTOMORPHOGENIC1 Is Required for the UV-B Response in Arabidopsis[W] , 2006, The Plant Cell Online.

[25]  A. Buckling,et al.  Bacteria-Phage Antagonistic Coevolution in Soil , 2011, Science.

[26]  C. Fankhauser,et al.  Light-regulated plant growth and development. , 2010, Current topics in developmental biology.

[27]  Edward J Oakeley,et al.  Interaction of COP1 and UVR8 regulates UV‐B‐induced photomorphogenesis and stress acclimation in Arabidopsis , 2009, The EMBO journal.

[28]  C. Fankhauser,et al.  Nuclear Accumulation of the Phytochrome A Photoreceptor Requires FHY1 , 2005, Current Biology.

[29]  David Creed,et al.  THE PHOTOPHYSICS AND PHOTOCHEMISTRY OF THE NEAR‐UV ABSORBING AMINO ACIDS–I. TRYPTOPHAN AND ITS SIMPLE DERIVATIVES , 1984 .

[30]  Gareth I. Jenkins,et al.  UV‐B Action Spectrum for UVR8‐Mediated HY5 Transcript Accumulation in Arabidopsis , 2009, Photochemistry and photobiology.

[31]  K. Müller,et al.  SnAvi – a new tandem tag for high-affinity protein-complex purification , 2010, Nucleic acids research.

[32]  W. Briggs,et al.  Role of root UV-B sensing in Arabidopsis early seedling development , 2008, Proceedings of the National Academy of Sciences.

[33]  Gareth I Jenkins,et al.  Signal transduction in responses to UV-B radiation. , 2009, Annual review of plant biology.

[34]  M. Dieterle,et al.  Functional analysis of EID1, an F-box protein involved in phytochrome A-dependent light signal transduction. , 2006, The Plant journal : for cell and molecular biology.