Anewly discovered human -globin gene

to the ease with which purified populations of hematopoietic cells are isolated. We hypothesized that human reticulocytes contain sufficient mRNA from the terminal stages of differentiation for the study of globin gene expression patterns in high throughput. Levels of globin mRNAdetection and differences in globin gene transcription between cord and adult blood were studied to determine the potential of this approach for clinical assessments of hemoglobinopathies and hemoglobin switching. Using oligonucleotide arrays, significantly different globin transcription patterns were found in cord and adult blood samples. Evidence for transcription of the major globin genes was clearly demonstrated. Surprisingly, we also identified transcription from the genomic region previously thought to encode the pseudo-2 gene. The source of that transcription is characterized in this report as a previously unrecognized globin gene.

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