Toxicological approaches to the metabolites of Fusaria. IX. Isolation of vomiting factor from moldy corn infected with Fusarium species.

A sample of moldy corn infected with Fusarium species was analyzed both chemically and biologically to elucidate the vomiting factor. With the aid of rabbit reticulocyte and ducking bioassays, 26 mg of deoxynivalenol, one of trichothecene mycotoxin, was isolated from 3.5 kg of the corn sample. The toxin was extracted with aqueous methanol and purified using solvent partitions and chromatographic separations.