MMHub, a database for the mulberry metabolome

Abstract Mulberry is an important economic crop plant and traditional medicine. It contains a huge array of bioactive metabolites such as flavonoids, amino acids, alkaloids and vitamins. Consequently, mulberry has received increasing attention in recent years. MMHub (version 1.0) is the first open public repository of mass spectra of small chemical compounds (<1000 Da) in mulberry leaves. The database contains 936 electrospray ionization tandem mass spectrometry (ESI-MS2) data and lists the specific distribution of compounds in 91 mulberry resources with two biological duplicates. ESI-MS2 data were obtained under non-standardized and independent experimental conditions. In total, 124 metabolites were identified or tentatively annotated and details of 90 metabolites with associated chemical structures have been deposited in the database. Supporting information such as PubChem compound information, molecular formula and metabolite classification are also provided in the MS2 spectral tag library. The MMHub provides important and comprehensive metabolome data for scientists working with mulberry. This information will be useful for the screening of quality resources and specific metabolites of mulberry. Database URL: https://biodb.swu.edu.cn/mmdb/

[1]  K. Shiwaku,et al.  Antioxidant flavonol glycosides in mulberry (Morus alba L.) leaves isolated based on LDL antioxidant activity , 2006 .

[2]  Hyun-bok Kim,et al.  UPLC-DAD-QTOF/MS Analysis of Flavonoids from 12 Varieties of Korean Mulberry Fruit , 2019, Journal of Food Quality.

[3]  A. Fernie,et al.  Exploring the Diversity of Plant Metabolism. , 2019, Trends in plant science.

[4]  Takao Shimizu,et al.  Basic analytical systems for lipidomics by mass spectrometry in Japan. , 2007, Methods in enzymology.

[5]  Jianbing Yan,et al.  Metabolome-based genome-wide association study of maize kernel leads to novel biochemical insights , 2014, Nature Communications.

[6]  Michal Holcapek,et al.  Comparison of negative ion electrospray mass spectra measured by seven tandem mass analyzers towards library formation. , 2008, Rapid communications in mass spectrometry : RCM.

[7]  Jingyuan Fu,et al.  The genetics of plant metabolism , 2006, Nature Genetics.

[8]  Shulei Qiu,et al.  Purification, characterization and in vitro and in vivo immune enhancement of polysaccharides from mulberry leaves , 2019, PloS one.

[9]  David S. Wishart,et al.  HMDB: a knowledgebase for the human metabolome , 2008, Nucleic Acids Res..

[10]  Wei Chen,et al.  Genome-wide association analyses provide genetic and biochemical insights into natural variation in rice metabolism , 2014, Nature Genetics.

[11]  Y. Kojima,et al.  Food-grade mulberry powder enriched with 1-deoxynojirimycin suppresses the elevation of postprandial blood glucose in humans. , 2007, Journal of agricultural and food chemistry.

[12]  A. Fernie,et al.  Genome-wide dissection of co-selected UV-B responsive pathways in the UV-B adaptation of qingke. , 2020, Molecular plant.

[13]  Xiaodong Zheng,et al.  Anthocyanin-rich mulberry fruit improves insulin resistance and protects hepatocytes against oxidative stress during hyperglycemia by regulating AMPK/ACC/mTOR pathway , 2017 .

[14]  R. P. Srivastava,et al.  Mulberry (Moms alba) leaves as human food: a new dimension of sericulture , 2003, International journal of food sciences and nutrition.

[15]  L. Xiong,et al.  A novel integrated method for large-scale detection, identification, and quantification of widely targeted metabolites: application in the study of rice metabolomics. , 2013, Molecular plant.

[16]  Z. Xiang,et al.  Definition of Eight Mulberry Species in the Genus Morus by Internal Transcribed Spacer-Based Phylogeny , 2015, PloS one.

[17]  A. Fernie,et al.  Metabolite profiling: from diagnostics to systems biology , 2004, Nature Reviews Molecular Cell Biology.

[18]  Heng Zhang,et al.  Purification of Flavonoids from Mulberry Leaves via High-Speed Counter-Current Chromatography , 2019, Processes.

[19]  M. Hirai,et al.  MassBank: a public repository for sharing mass spectral data for life sciences. , 2010, Journal of mass spectrometry : JMS.

[20]  A. Y. Chen,et al.  A review of the dietary flavonoid, kaempferol on human health and cancer chemoprevention. , 2013, Food chemistry.

[21]  S. Cho,et al.  Enhancement of 1-deoxynojirimycin content and α-glucosidase inhibitory activity in mulberry leaf using various fermenting microorganisms isolated from Korean traditional fermented food , 2014, Biotechnology and Bioprocess Engineering.

[22]  Xiao-dong Lin,et al.  Metabolic changes in the midgut of Eri silkworm after Oral administration of 1-deoxynojirimycin: A 1H-NMR-based metabonomic study , 2017, PloS one.

[23]  R. Abagyan,et al.  METLIN: A Metabolite Mass Spectral Database , 2005, Therapeutic drug monitoring.

[24]  Saburo Hara,et al.  Mulberry leaf extract prevents amyloid beta-peptide fibril formation and neurotoxicity , 2007, Neuroreport.

[25]  Guojun Yang,et al.  Draft genome sequence of the mulberry tree Morus notabilis , 2013, Nature Communications.

[26]  K. Thakur,et al.  Metabolic Effect of 1-Deoxynojirimycin from Mulberry Leaves on db/db Diabetic Mice Using Liquid Chromatography-Mass Spectrometry Based Metabolomics. , 2017, Journal of agricultural and food chemistry.

[27]  Jung Eun Lee,et al.  Anti-inflammatory activity of mulberry leaf extract through inhibition of NF-κB , 2013 .

[28]  Christian Gieger,et al.  Genetic variation in metabolic phenotypes: study designs and applications , 2012, Nature Reviews Genetics.

[29]  A. Aharoni,et al.  Transcriptome and Metabolic Profiling Provides Insights into Betalain Biosynthesis and Evolution in Mirabilis jalapa. , 2018, Molecular plant.

[30]  J. Hohmann,et al.  Metabolic Effects of Mulberry Leaves: Exploring Potential Benefits in Type 2 Diabetes and Hyperuricemia , 2013, Evidence-based complementary and alternative medicine : eCAM.

[31]  Mark Stitt,et al.  Recommendations for Reporting Metabolite Data[W] , 2011, Plant Cell.

[32]  Xiangrong Li,et al.  Hypolipidemic effect of flavonoids from mulberry leaves in triton WR-1339 induced hyperlipidemic mice. , 2007, Asia Pacific journal of clinical nutrition.

[33]  Mark Stitt,et al.  Characterization of a recently evolved flavonol-phenylacyltransferase gene provides signatures of natural light selection in Brassicaceae , 2016, Nature Communications.

[34]  Tian Li,et al.  MorusDB: a resource for mulberry genomics and genome biology , 2014, Database J. Biol. Databases Curation.