Potential Theranostics Application of Bio-Synthesized Silver Nanoparticles (4-in-1 System)

In this report, we have designed a simple and efficient green chemistry approach for the synthesis of colloidal silver nanoparticles (b-AgNPs) that is formed by the reduction of silver nitrate (AgNO3) solution using Olax scandens leaf extract. The colloidal b-AgNPs, characterized by various physico-chemical techniques exhibit multifunctional biological activities (4-in-1 system). Firstly, bio-synthesized silver nanoparticles (b-AgNPs) shows enhanced antibacterial activity compared to chemically synthesize silver nanoparticles (c-AgNPs). Secondly, b-AgNPs show anti-cancer activities to different cancer cells (A549: human lung cancer cell lines, B16: mouse melanoma cell line & MCF7: human breast cancer cells) (anti-cancer). Thirdly, these nanoparticles are biocompatible to rat cardiomyoblast normal cell line (H9C2), human umbilical vein endothelial cells (HUVEC) and Chinese hamster ovary cells (CHO) which indicates the future application of b-AgNPs as drug delivery vehicle. Finally, the bio-synthesized AgNPs show bright red fluorescence inside the cells that could be utilized to detect the localization of drug molecules inside the cancer cells (a diagnostic approach). All results together demonstrate the multifunctional biological activities of bio-synthesized AgNPs (4-in-1 system) that could be applied as (i) anti-bacterial & (ii) anti-cancer agent, (iii) drug delivery vehicle, and (iv) imaging facilitator. To the best of our knowledge, there is not a single report of biosynthesized AgNPs that demonstrates the versatile applications (4-in-1 system) towards various biomedical applications. Additionally, a plausible mechanistic approach has been explored for the synthesis of b-AgNPs and its anti-bacterial as well as anti-cancer activity. We strongly believe that bio-synthesized AgNPs will open a new direction towards various biomedical applications in near future.

[1]  M. Ayyanar,et al.  Traditional uses of medicinal plants among the tribal people in Theni District (Western Ghats), Southern India , 2011 .

[2]  Jing Kong,et al.  Antibacterial activity of graphite, graphite oxide, graphene oxide, and reduced graphene oxide: membrane and oxidative stress. , 2011, ACS nano.

[3]  M. Hitt,et al.  Re-expression of TSLC1 in a non-small-cell lung cancer cell line induces apoptosis and inhibits tumor growth , 2004, Oncogene.

[4]  A. Awad,et al.  Peanuts as a Source of β-Sitosterol, a Sterol With Anticancer Properties , 2000 .

[5]  Shiv Shankar,et al.  Controlling the Optical Properties of Lemongrass Extract Synthesized Gold Nanotriangles and Potential Application in Infrared-Absorbing Optical Coatings , 2005 .

[6]  G. Blanchard,et al.  Formation of gold nanoparticles using amine reducing agents. , 2006, Langmuir : the ACS journal of surfaces and colloids.

[7]  D. Mukhopadhyay,et al.  Pro‐angiogenic Properties of Europium(III) Hydroxide Nanorods , 2008 .

[8]  D. Mukhopadhyay,et al.  Targeted delivery of gemcitabine to pancreatic adenocarcinoma using cetuximab as a targeting agent. , 2008, Cancer research.

[9]  Martyn Poliakoff,et al.  The 24 Principles of Green Engineering and Green Chemistry: “IMPROVEMENTS PRODUCTIVELY” , 2008 .

[10]  Joana M. Xavier,et al.  The role of p53 in apoptosis. , 2010, Discovery medicine.

[11]  Y. Park,et al.  Antibacterial Activity and Mechanism of Action of the Silver Ion in Staphylococcus aureus and Escherichia coli , 2008, Applied and Environmental Microbiology.

[12]  R. Koren,et al.  The role of reactive oxygen species in the anticancer activity of vitamin D. , 2003, Recent results in cancer research. Fortschritte der Krebsforschung. Progres dans les recherches sur le cancer.

[13]  I. Tannock,et al.  Acid pH in tumors and its potential for therapeutic exploitation. , 1989, Cancer research.

[14]  S. Ignacimuthu,et al.  Antimicrobial activity of some ethnomedicinal plants used by Paliyar tribe from Tamil Nadu, India , 2006, BMC complementary and alternative medicine.

[15]  Daniel I. C. Wang,et al.  Identification of active biomolecules in the high-yield synthesis of single-crystalline gold nanoplates in algal solutions. , 2007, Small.

[16]  S. Gurunathan,et al.  Cytotoxicity of Biologically Synthesized Silver Nanoparticles in MDA-MB-231 Human Breast Cancer Cells , 2013, BioMed research international.

[17]  L. Tran,et al.  Synthesis, characterization, antibacterial and antiproliferative activities of monodisperse chitosan- based silver nanoparticles , 2010 .

[18]  U. Banerjee,et al.  Biosynthesis of silver nanoparticles: Elucidation of prospective mechanism and therapeutic potential. , 2014, Journal of colloid and interface science.

[19]  Michael S Strano,et al.  Reactive oxygen species driven angiogenesis by inorganic nanorods. , 2011, Nano letters.

[20]  A. Sironmani,et al.  Silver Nanoparticles – Universal Multifunctional Nanoparticles for Bio Sensing, Imaging for Diagnostics and Targeted Drug Delivery for Therapeutic Applications , 2011 .

[21]  Y. Ju,et al.  beta-Sitosterol, beta-Sitosterol Glucoside, and a Mixture of beta-Sitosterol and beta-Sitosterol Glucoside Modulate the Growth of Estrogen-Responsive Breast Cancer Cells In Vitro and in Ovariectomized Athymic Mice. , 2004, The Journal of nutrition.

[22]  E. Holland,et al.  E4BP4 facilitates glucocorticoid-evoked apoptosis of human leukemic CEM cells via upregulation of Bim , 2011, Journal of molecular signaling.

[23]  L. Zhang,et al.  Nanoparticles in Medicine: Therapeutic Applications and Developments , 2008, Clinical pharmacology and therapeutics.

[24]  F. Porta,et al.  Gold nanoparticles prepared using cape aloe active components. , 2009, Langmuir : the ACS journal of surfaces and colloids.

[25]  Qingshan Shi,et al.  Antibacterial activity and mechanism of silver nanoparticles on Escherichia coli , 2009, Applied Microbiology and Biotechnology.

[26]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[27]  H. White,et al.  Electrochemistry of Sulfur Adlayers on the Low-Index Faces of Silver , 1996 .

[28]  S. Godet,et al.  Synthesis of Silver Nanoparticles by Chemical Reduction Method and Their Antibacterial Activity , 2008 .

[29]  B. Sreedhar,et al.  Potential therapeutic and diagnostic applications of one-step in situ biosynthesized gold nanoconjugates (2-in-1 system) in cancer treatment , 2013 .

[30]  Zhong-Yu Duan,et al.  Anti-bacterial and in vivo tumor treatment by reactive oxygen species generated by magnetic nanoparticles. , 2013, Journal of materials chemistry. B.

[31]  Z. Gong,et al.  Toxicity of silver nanoparticles in zebrafish models , 2008, Nanotechnology.

[32]  Milan Kolar,et al.  Antibacterial activity and toxicity of silver – nanosilver versus ionic silver , 2011 .

[33]  S. Banerjee,et al.  A proteomic view of isoproterenol induced cardiac hypertrophy: Prohibitin identified as a potential biomarker in rats , 2013, Journal of Translational Medicine.

[34]  C. Patra,et al.  A luminescent nanoporous hybrid material based drug delivery system showing excellent theranostics potential for cancer. , 2013, Chemical communications.

[35]  B. Sreedhar,et al.  Green chemistry approach for the synthesis and stabilization of biocompatible gold nanoparticles and their potential applications in cancer therapy , 2012, Nanotechnology.

[36]  S. Kedzierska,et al.  The role of DnaK/DnaJ and GroEL/GroES systems in the removal of endogenous proteins aggregated by heat‐shock from Escherichia coli cells , 1999, FEBS letters.

[37]  M. Ahamed,et al.  Silver nanoparticle applications and human health. , 2010, Clinica chimica acta; international journal of clinical chemistry.

[38]  M. Ueno,et al.  Redox control of cell death. , 2002, Antioxidants & redox signaling.

[39]  G. Georgiou,et al.  The many faces of glutathione in bacteria. , 2006, Antioxidants & redox signaling.

[40]  M. El-Sayed,et al.  Gold and silver nanoparticles in sensing and imaging: sensitivity of plasmon response to size, shape, and metal composition. , 2006, The journal of physical chemistry. B.

[41]  Cristina Rodríguez-Padilla,et al.  Antitumor activity of colloidal silver on MCF-7 human breast cancer cells , 2010, Journal of experimental & clinical cancer research : CR.

[42]  A. Hameed,et al.  Antibacterial Characterization of Silver Nanoparticles against E. Coli ATCC-15224 , 2009 .

[43]  L. Galluzzi,et al.  Mechanisms of cytochrome c release from mitochondria , 2006, Cell Death and Differentiation.

[44]  S. Prabhu,et al.  Silver nanoparticles: mechanism of antimicrobial action, synthesis, medical applications, and toxicity effects , 2012, International Nano Letters.

[45]  A B Awad,et al.  Peanuts as a source of beta-sitosterol, a sterol with anticancer properties. , 2000, Nutrition and cancer.

[46]  Krishnendu Pal,et al.  Zinc oxide nanoflowers make new blood vessels. , 2012, Nanoscale.

[47]  Hyun‐Seok Kim,et al.  Wound healing and antibacterial activities of chondroitin sulfate- and acharan sulfate-reduced silver nanoparticles , 2013, Nanotechnology.

[48]  J. Zweier,et al.  Cancer chemopreventive oltipraz generates superoxide anion radical. , 2005, Archives of biochemistry and biophysics.

[49]  K. Tománková,et al.  The targeted antibacterial and antifungal properties of magnetic nanocomposite of iron oxide and silver nanoparticles. , 2011, Biomaterials.

[50]  Y. Ju,et al.  β-Sitosterol, β-Sitosterol Glucoside, and a Mixture of β-Sitosterol and β-Sitosterol Glucoside Modulate the Growth of Estrogen-Responsive Breast Cancer Cells In Vitro and in Ovariectomized Athymic Mice , 2004 .

[51]  Robert A. Keyzers,et al.  Reactive oxygen species mediated apoptosis of esophageal cancer cells induced by marine triprenyl toluquinones and toluhydroquinones , 2007, Molecular Cancer Therapeutics.

[52]  Biju Jacob,et al.  Toxicity and antibacterial assessment of chitosancoated silver nanoparticles on human pathogens and macrophage cells , 2012, International journal of nanomedicine.

[53]  A. Ferraris,et al.  Predominant role of catalase in the disposal of hydrogen peroxide within human erythrocytes. , 1996, Blood.

[54]  Chad A. Mirkin,et al.  Gold nanoparticles for biology and medicine. , 2010, Angewandte Chemie.

[55]  Daniella Yeheskely-Hayon,et al.  High levels of reactive oxygen species in gold nanoparticle-targeted cancer cells following femtosecond pulse irradiation , 2013, Scientific Reports.

[56]  Rajender S Varma,et al.  Beet juice-induced green fabrication of plasmonic AgCl/Ag nanoparticles. , 2012, ChemSusChem.

[57]  P. Wei,et al.  Glucose-Regulated Protein 78 (GRP78) Mediated the Efficacy to Curcumin Treatment on Hepatocellular Carcinoma , 2011, Annals of Surgical Oncology.

[58]  Arnab Roy,et al.  Characterization of enhanced antibacterial effects of novel silver nanoparticles , 2007, Nanotechnology.

[59]  Jin Won Hyun,et al.  Silver nanoparticles induce oxidative cell damage in human liver cells through inhibition of reduced glutathione and induction of mitochondria-involved apoptosis. , 2011, Toxicology letters.

[60]  Aruna Jyothi Kora,et al.  Superior bactericidal activity of SDS capped silver nanoparticles: Synthesis and characterization , 2009 .

[61]  S. Nie,et al.  In vivo cancer targeting and imaging with semiconductor quantum dots , 2004, Nature Biotechnology.

[62]  S Gurudeeban,et al.  Biomedical potential of silver nanoparticles synthesized from calli cells of Citrullus colocynthis (L.) Schrad. , 2011, Journal of nanobiotechnology.

[63]  P. Alivisatos.  The use of nanocrystals in biological detection , 2004, Nature Biotechnology.

[64]  O. Salata,et al.  Applications of nanoparticles in biology and medicine , 2004, Journal of nanobiotechnology.

[65]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[66]  N. Mei,et al.  Silver nanoparticle‐induced mutations and oxidative stress in mouse lymphoma cells , 2012, Environmental and molecular mutagenesis.

[67]  Ramanathan Vaidyanathan,et al.  Antiangiogenic properties of silver nanoparticles. , 2009, Biomaterials.

[68]  Satish K. Nune,et al.  Soybeans as a phytochemical reservoir for the production and stabilization of biocompatible gold nanoparticles. , 2008, Small.

[69]  Chad A Mirkin,et al.  Gold nanoparticles for biology and medicine. , 2010, Angewandte Chemie.

[70]  K. Song,et al.  Antibacterial activity of silver nanoparticles prepared by a chemical reduction method , 2010 .

[71]  T. Hotokebuchi,et al.  Antibacterial properties of nanostructured silver titanate thin films formed on a titanium plate. , 2009, Journal of biomedical materials research. Part A.

[72]  Chunrong Yu,et al.  Synergistic antitumour activity of sorafenib in combination with tetrandrine is mediated by reactive oxygen species (ROS)/Akt signaling , 2013, British Journal of Cancer.

[73]  Ganesan Singaravelu,et al.  Silver, gold and bimetallic nanoparticles production using single-cell protein (Spirulina platensis) Geitler , 2008, Journal of Materials Science.

[74]  G Thippeswamy,et al.  Octacosanol isolated from Tinospora cordifolia downregulates VEGF gene expression by inhibiting nuclear translocation of NF-B and its DNA binding activity. , 2008, European journal of pharmacology.

[75]  N. Savithramma.  BIO-PROSPECTING AND DOCUMENTATION OF TRADITIONAL MEDICINAL PLANTS USED TO TREAT ITCHING, PSORIASIS AND WOUNDS BY ETHNIC GROUPS OF KURNOOL DISTRICT, ANDHRA PRADESH, INDIA. , 2012 .

[76]  M. Yacamán,et al.  The bactericidal effect of silver nanoparticles , 2005, Nanotechnology.

[77]  E. Hoek,et al.  A review of the antibacterial effects of silver nanomaterials and potential implications for human health and the environment , 2010 .