Calculus removal and the prevention of its formation.

Periodontitis is strongly associated with the presence of dental calculus on root surfaces. Although the rough calculus surface may not in itself induce inflammation in the adjacent periodontal tissues, dental calculus serves as an ideal substrate for subgingival microbial colonization. Therefore, causerelated anti-infective therapy aims to eliminate the microbial biofilm and calcified deposits from the diseased root surfaces by means of root surface debridement. Over the past 50 years, a large number of clinical and laboratory studies have been performed to determine the efficacy of calculus removal from diseased root surfaces by various methods. These studies aimed to determine whether complete removal of subgingival calculus by root surface debridement is possible. They also evaluated the importance of operator experience in the effectiveness of calculus removal. Possible differences in efficacy between hand tools and power-driven instruments or lasers have been investigated. The impact of tooth and site characteristics, such as probing depths, tooth type, tooth surfaces and furcation areas, has also been evaluated. In addition, side-effects such as unintentional root substance removal and patient discomfort have been assessed. This review focuses on the composition and formation of calculus, its significance for the disease process, the methods available for calculus removal, and prevention of its formation.

[1]  Y. K. Chan,et al.  Comparison of periodontal pocket penetration by conventional and microultrasonic inserts. , 1999, Journal of Clinical Periodontology.

[2]  F. Grases,et al.  Effects of phytate and pyrophosphate on brushite and hydroxyapatite crystallization , 2000, Urological Research.

[3]  H. R. Hitz,et al.  Investigations on the mode of action of Triclosan, a broad spectrum antimicrobial agent. , 1974, Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie und Parasitologie.

[4]  J. Waerhaug The gingival pocket; anatomy, pathology, deepening and elimination. , 1952, Odontologisk tidskrift.

[5]  B. Gustafsson,et al.  Dental calculus in germfree rats. , 1962, Acta odontologica Scandinavica.

[6]  R. Genco Assessment of risk of periodontal disease. , 1994, Compendium (Newtown, Pa.). Supplement.

[7]  J. Bernimoulin,et al.  Effect of diphosphonate on human supragingival calculus. , 1970, Helvetica odontologica acta.

[8]  J. Clarkson,et al.  A randomised controlled trial to explore attitudes to routine scale and polish and compare manual versus ultrasonic scaling in the general dental service in Scotland [ISRCTN99609795] , 2005, BMC oral health.

[9]  R. Yukna,et al.  Clinical evaluation of the speed and effectiveness of subgingival calculus removal on single-rooted teeth with diamond-coated ultrasonic tips. , 1997, Journal of periodontology.

[10]  R. Genco,et al.  Dental Plaque and Calculus: Risk Indicators for Their Formation , 1992, Journal of dental research.

[11]  H. Worthington,et al.  The detection of Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis and Prevotella intermedia using an ELISA in an adolescent population with early periodontitis. , 1997, Journal of clinical periodontology.

[12]  P. M. de Freitas,et al.  Er:YAG Laser, ultrasonic system, and curette produce different profiles on dentine root surfaces: an in vitro study. , 2008, Photomedicine and laser surgery.

[13]  A. Aoki,et al.  In vitro studies on laser scaling of subgingival calculus with an erbium:YAG laser. , 1994, Journal of periodontology.

[14]  D. J. White,et al.  The comparative clinical efficacy of pyrophosphate/triclosan, copolymer/triclosan and zinc citrate/triclosan dentifrices for the reduction of supragingival calculus formation. , 1997, The Journal of clinical dentistry.

[15]  J. Featherstone Caries detection and prevention with laser energy. , 2000, Dental clinics of North America.

[16]  J. Waerhaug The Source of Mineral Salts in Subgingival Calculus , 1955, Journal of dental research.

[17]  M. Gilthorpe,et al.  Prevalence and extent of lifetime cumulative attachment loss (LCAL) at different thresholds and associations with clinical variables: changes in a population of young male military recruits over 3 years. , 2001, Journal of clinical periodontology.

[18]  I. Mandel,et al.  Calculus update: prevalence, pathogenicity and prevention. , 1995, Journal of the American Dental Association.

[19]  Absence of histologic signs of chronic inflammation following closed subgingival scaling and root planing using the dental endoscope: human biopsies - a pilot study. , 2008, Journal of periodontology.

[20]  A. Aoki,et al.  In vitro evaluation of Er:YAG laser scaling of subgingival calculus in comparison with ultrasonic scaling. , 2000, Journal of periodontal research.

[21]  Y. Açil,et al.  Efficacy of subgingival calculus removal with Er:YAG laser compared to mechanical debridement: an in situ study. , 2003, Journal of clinical periodontology.

[22]  G. S. Ingram,et al.  The Oral Disposition of Zinc Following the Use of an Anticalculus Toothpaste Containing 0.5% Zinc Citrate , 1988, The Journal of pharmacy and pharmacology.

[23]  K. Selvig Attachment of plaque and calculus to tooth surfaces. , 1970, Journal of periodontal research.

[24]  K. Yokoyama,et al.  Effects of erbium, chromium:YSGG laser irradiation on root surface: morphological and atomic analytical studies. , 2001, Journal of clinical laser medicine & surgery.

[25]  D. J. White,et al.  Dental calculus: recent insights into occurrence, formation, prevention, removal and oral health effects of supragingival and subgingival deposits. , 1997, European journal of oral sciences.

[26]  I. Ishikawa,et al.  In vitro effectiveness of a newly-designed ultrasonic scaler tip for furcation areas. , 1989, Journal of periodontology.

[27]  A. Aoki,et al.  Lasers in nonsurgical periodontal therapy. , 2004, Periodontology 2000.

[28]  T. O'Leary,et al.  The effectiveness of hand versus ultrasonic instrumentation in open flap root planing. , 1984, Journal of periodontology.

[29]  Chun-Chan Ting,et al.  Effects of Er,Cr:YSGG laser irradiation on the root surface: morphologic analysis and efficiency of calculus removal. , 2007, Journal of periodontology.

[30]  H. Schroeder,et al.  Formation and inhibition of dental calculus. , 1969, Journal of periodontology.

[31]  R. Caffesse,et al.  Scaling and root planing with and without periodontal flap surgery. , 1986, Journal of clinical periodontology.

[32]  L. Matsson,et al.  Marginal bone loss in the primary dentition. A survey of 7-9-year-old children in Sweden. , 1994, Journal of clinical periodontology.

[33]  R Hickel,et al.  The effect of working parameters on root substance removal using a piezoelectric ultrasonic scaler in vitro. , 1998, Journal of clinical periodontology.

[34]  A. Parashis,et al.  Calculus removal from multirooted teeth with and without surgical access. II. Comparison between external and furcation surfaces and effect of furcation entrance width. , 1993, Journal of clinical periodontology.

[35]  P. Preshaw,et al.  The use of a linear oscillating device in periodontal treatment: a review. , 2008, Journal of clinical periodontology.

[36]  F. Grases,et al.  Anticalculus effect of a triclosan mouthwash containing phytate: a double-blind, randomized, three-period crossover trial. , 2009, Journal of periodontal research.

[37]  T. O'Leary,et al.  The effect of retained subgingival calculus on healing after flap surgery. , 1988, Journal of periodontology.

[38]  M. Beuchat,et al.  A comparative in vitro study of a magnetostrictive and a piezoelectric ultrasonic scaling instrument. , 2001, Journal of clinical periodontology.

[39]  A. Braun,et al.  Efficiency of the Vector -system compared with conventional subgingival debridement in vitro and in vivo. , 2006, Journal of clinical periodontology.

[40]  L. Hammarström,et al.  An enzyme histochemical study of dental plaque and calculus. , 1982, Acta odontologica Scandinavica.

[41]  I. Needleman,et al.  A guide to systematic reviews. , 2002, Journal of clinical periodontology.

[42]  M. Lennon,et al.  A two-year longitudinal study of early periodontitis in 14- to 16-year-old schoolchildren. , 1986, Community dental health.

[43]  F. Nichols,et al.  Porphyromonas gingivalis lipids and diseased dental tissues. , 2006, Oral microbiology and immunology.

[44]  J. Otomo-Corgel,et al.  The effectiveness of scaling and root planing with curets designed for deep pockets. , 1992, Journal of periodontology.

[45]  Albert Mehl,et al.  Subgingival calculus detection with fluorescence induced by 655 nm InGaAsP diode laser radiation. , 2002, Journal of periodontology.

[46]  T. Kocher,et al.  Substance loss caused by scaling with different sonic scaler inserts--an in vitro study. , 2008, Journal of clinical periodontology.

[47]  A. Braun,et al.  Subjective intensity of pain during ultrasonic supragingival calculus removal. , 2007, Journal of clinical periodontology.

[48]  B. Simonet,et al.  Dietary myo-inositol hexaphosphate prevents dystrophic calcifications in soft tissues: a pilot study in Wistar rats. , 2004, Life sciences.

[49]  M. Ash,et al.  The effectiveness of subgingival scaling and root planing in calculus removal. , 1981, Journal of periodontology.

[50]  P. Grøn,et al.  Human dental calculus. Inorganic chemical and crystallographic composition. , 1967, Archives of oral biology.

[51]  R. Gerlach,et al.  Anticalculus efficacy and safety of a novel whitening dentifrice containing sodium hexametaphosphate: a controlled six-month clinical trial. , 2002, The Journal of clinical dentistry.

[52]  V. Clerehugh,et al.  The effect of subgingival calculus on the validity of clinical probing measurements. , 1996, Journal of dentistry.

[53]  J. Hedderich,et al.  Significant influence of scaler tip design on root substance loss resulting from ultrasonic scaling: a laserprofilometric in vitro study. , 2004, Journal of clinical periodontology.

[54]  R. Oppermann,et al.  Thiol groups and reduced acidogenicity of dental plaque in the presence of metal ions in vivo. , 1980, Scandinavian journal of dental research.

[55]  T. Karring,et al.  Role of "diseased" root cementum in healing following treatment of periodontal disease. A clinical study. , 1988, Journal of clinical periodontology.

[56]  A. Braun,et al.  Efficiency of the VectorTM‐system compared with conventional subgingival debridement in vitro and in vivo , 2006 .

[57]  Kopczyk Ra,et al.  The attachment of calculus to root planed surfaces. , 1968 .

[58]  D. Adams Calculus-Inhibition Agents: a Review of Recent Clinical Trials , 1995 .

[59]  R. Hickel,et al.  Working parameters of a sonic scaler influencing root substance removal in vitro , 1997, Clinical Oral Investigations.

[60]  I. Mandel Biochemical aspects of calculus formation. , 1969, Journal of periodontal research. Supplement.

[61]  R. Sognnaes,et al.  Electronmicroscopy of Dental Calculus , 1960, Science.

[62]  P. Soparkar,et al.  Reduced formation of supragingival calculus with use of fluoride-zinc chloride dentifrice. , 1987, Journal of the American Dental Association.

[63]  H. Löe,et al.  Gingival state and dental calculus in early-onset periodontitis. , 1996, Journal of periodontology.

[64]  C. Schwahn,et al.  A new ultrasonic device in maintenance therapy: perception of pain and clinical efficacy. , 2005, Journal of clinical periodontology.

[65]  H. Löe,et al.  Gingival inflammation and subgingival calculus as determinants of disease progression in early-onset periodontitis. , 1998, Journal of clinical periodontology.

[66]  L. Hammarström,et al.  A comparative, scanning electron microscopic study of supragingival and subgingival calculus. , 1980, Journal of periodontology.

[67]  R. Hickel,et al.  Influence of parameters on root surface roughness following treatment with a magnetostrictive ultrasonic scaler: an in vitro study. , 2004, Journal of periodontology.

[68]  H. Worthington,et al.  Site progression of loss of attachment over 5 years in 14- to 19-year-old adolescents. , 2005, Journal of clinical periodontology.

[69]  G. Ong,et al.  Periodontal reasons for tooth loss in an Asian population. , 1996, Journal of clinical periodontology.

[70]  J. Mellonig,et al.  Scaling and root planing efficacy in multirooted teeth. , 1989, Journal of periodontology.

[71]  A. Parashis,et al.  Calculus removal from multirooted teeth with and without surgical access. (I). Efficacy on external and furcation surfaces in relation to probing depth. , 1993, Journal of clinical periodontology.

[72]  G. Isacsson,et al.  A quantitative microradiographic study of mineral content of supragingival and subgingival dental calculus. , 1984, Scandinavian journal of dental research.

[73]  K. Matsumoto,et al.  Effects of erbium,chromium:YSGG laser irradiation on canine mandibular bone. , 2001, Journal of periodontology.

[74]  D. Breininger,et al.  Comparative effectiveness of ultrasonic and hand scaling for the removal of subgingival plaque and calculus. , 1987, Journal of periodontology.

[75]  A. Braun,et al.  Efficiency of subgingival calculus removal with the Vector-system compared to ultrasonic scaling and hand instrumentation in vitro. , 2005, Journal of periodontal research.

[76]  H. Shinoda,et al.  Inhibitory effect of a novel bisphosphonate, TRK-530, on dental calculus formation in rats. , 2004, Journal of periodontology.

[77]  S. C. Lea,et al.  Advances in power driven pocket/root instrumentation. , 2008, Journal of clinical periodontology.

[78]  F. Schwarz,et al.  Influence of fluorescence-controlled Er:YAG laser radiation, the Vector system and hand instruments on periodontally diseased root surfaces in vivo. , 2006, Journal of clinical periodontology.

[79]  P. Robertson,et al.  Calculus removal by scaling/root planing with and without surgical access. , 1987, Journal of periodontology.

[80]  C. Gürgan,et al.  Distribution of different morphologic types of subgingival calculus on proximal root surfaces. , 2005, Quintessence international.

[81]  J. Waerhaug,et al.  Incidence of clinical manifestations of periodontal disease in light of oral hygiene and calculus formation. , 1958, Journal of the American Dental Association.

[82]  J. Pratten,et al.  Study of bacterial viability within human supragingival dental calculus. , 2004, Journal of periodontology.

[83]  P. Bartold,et al.  Use of the Vector scaling unit in supportive periodontal therapy: a subjective patient evaluation. , 2005, Journal of clinical periodontology.

[84]  C. Bleiwas,et al.  Zinc effect on the in vitro formation of calcium phosphates: relevance to clinical inhibition of calculus formation. , 1999, American journal of dentistry.

[85]  W. Teughels,et al.  Innovations in non-surgical periodontal therapy: Consensus Report of the Sixth European Workshop on Periodontology. , 2008, Journal of clinical periodontology.

[86]  B. Mealey,et al.  The clinical effectiveness of open versus closed scaling and root planing on multi-rooted teeth. , 1993, Journal of periodontology.

[87]  Matthias Frentzen,et al.  Evaluation of selective calculus removal by a fluorescence feedback-controlled Er:YAG laser in vitro. , 2007, Journal of clinical periodontology.

[88]  J. Bergström Tobacco smoking and subgingival dental calculus. , 2005, Journal of clinical periodontology.

[89]  P. R. Sherman,et al.  The effectiveness of subgingival scaling and root planning. I. Clinical detection of residual calculus. , 1990, Journal of periodontology.

[90]  T. Kocher,et al.  Pain during prophylaxis treatment elicited by two power-driven instruments. , 2005, Journal of clinical periodontology.

[91]  F. V. Ribeiro,et al.  Root surface defect produced by hand instruments and ultrasonic scaler with different power settings: an in vitro study. , 2009, Brazilian dental journal.

[92]  O. P. Sturzenberger,et al.  Reduction of dental calculus in humans through the use of a dentifrice containing a crystal-growth inhibitor. , 1971, Journal of periodontology.

[93]  N. Lang,et al.  Clinical and microbiological changes associated with an altered subgingival environment induced by periodontal pocket reduction. , 1995, Journal of clinical periodontology.

[94]  Xiaogu Wang,et al.  In vivo study of the healing processes that occur in the jaws of rabbits following perforation by an Er,Cr:YSGG laser , 2005, Lasers in Medical Science.

[95]  M. Frentzen,et al.  The possibility of detecting subgingival calculus by laser-fluorescence in vitro , 2003, Lasers in Medical Science.

[96]  M. Kishida,et al.  A comparison of root surface instrumentation using two piezoelectric ultrasonic scalers and a hand scaler in vivo. , 2007, Journal of periodontal research.

[97]  V Clerehugh,et al.  Subgingival calculus: where are we now? A comparative review. , 2000, Journal of dentistry.

[98]  O. P. Sturzenberger,et al.  The rate of calculus formation in adults. , 1968, Journal of periodontology.

[99]  T. Karring,et al.  Role of "diseased" root cementum in healing following treatment of periodontal disease. An experimental study in the dog. , 1986, Journal of periodontal research.

[100]  C. Cobb Clinical significance of non-surgical periodontal therapy: an evidence-based perspective of scaling and root planing. , 2002, Journal of clinical periodontology.

[101]  D. J. White,et al.  In vitro studies of the anticalculus efficacy of a sodium hexametaphosphate whitening dentifrice. , 2002, The Journal of Clinical Dentistry.

[102]  R. Schenk,et al.  Absence of histologic signs of chronic inflammation following closed subgingival scaling and root planing using the dental endoscope: human biopsies - a pilot study. , 2008, Journal of periodontology.

[103]  R. Gellin,et al.  The Effectiveness of the Titan‐S Sonic Sealer Versus Curettes in the Removal of Subgingival Calculus , 1986 .

[104]  A. Aoki,et al.  Laser application in non-surgical periodontal therapy: a systematic review. , 2008, Journal of clinical periodontology.

[105]  R. Kopczyk,et al.  The attachment of calculus to root planed surfaces. , 1968, Periodontics.

[106]  F. Schwarz,et al.  In vivo and in vitro effects of an Er:YAG laser, a GaAlAs diode laser, and scaling and root planing on periodontally diseased root surfaces: A comparative histologic study , 2003, Lasers in surgery and medicine.

[107]  R. Hickel,et al.  Safety and efficiency of novel sonic scaler tips in vitro. , 2003, Journal of clinical periodontology.

[108]  M. Ryder,et al.  The effects of subgingival calculus on the clinical outcomes of locally-delivered controlled-release doxycycline compared to scaling and root planing. , 2002, Journal of clinical periodontology.

[109]  R. Gellin,et al.  The effectiveness of the Titan-S sonic scaler versus curettes in the removal of subgingival calculus. A human surgical evaluation. , 1986, Journal of periodontology.

[110]  R. Davies,et al.  A review of calculus clinical efficacy studies. , 1993, The Journal of clinical dentistry.

[111]  T. Modéer,et al.  Risk factors and risk indicators in relation to incipient alveolar bone loss in Swedish 19-year-olds , 2008, Acta odontologica Scandinavica.

[112]  A. Gaffar,et al.  The effect of triclosan on mediators of gingival inflammation. , 1995, Journal of clinical periodontology.

[113]  M. Little,et al.  Dental Calculus Composition. 2. Subgingival Calculus: Ash, Calcium, Phosphorus, and Sodium , 1964, Journal of dental research.

[114]  A. Kingman,et al.  Gingival recession, gingival bleeding, and dental calculus in adults 30 years of age and older in the United States, 1988-1994. , 1999, Journal of periodontology.

[115]  H. Horowitz,et al.  A clinical trial of a calculus-inhibitory dentifrice. , 1974, Journal of periodontology.

[116]  I. Roeder,et al.  In vitro, clinical, and microbiological evaluation of a linear oscillating device for scaling and root planing. , 2005, Journal of periodontology.

[117]  R. Caffesse,et al.  Effectiveness of subgingival scaling and root planing: single versus multiple episodes of instrumentation. , 1996, Journal of periodontology.

[118]  M. Listgarten,et al.  Electron microscopic evidence of a cellular attachment between junctional epithelium and dental calculus. , 1973, Journal of periodontal research.

[119]  A. Heinecke,et al.  A systematic review of efficacy of machine-driven and manual subgingival debridement in the treatment of chronic periodontitis. , 2002, Journal of clinical periodontology.

[120]  A. Moritz,et al.  Treatment of periodontal pockets with a diode laser , 1998, Lasers in surgery and medicine.

[121]  D. Birkhed,et al.  The release of vitamin C from chewing gum and its effects on supragingival calculus formation. , 2005, European journal of oral sciences.

[122]  R. Fitzgerald,et al.  ELECTRON MICROSCOPIC OBSERVATIONS OF DENTAL CALCULUS IN GERMFREE AND CONVENTIONAL RATS. , 1964, Archives of oral biology.

[123]  R. Davies,et al.  Anticalculus efficacy of three dentifrices. , 1995, American journal of dentistry.

[124]  J. Mellonig,et al.  Scaling and root planing effectiveness: the effect of root surface access and operator experience. , 1989, Journal of periodontology.

[125]  T. Kocher,et al.  Effectiveness of subgingival instrumentation with power-driven instruments in the hands of experienced and inexperienced operators. A study on manikins. , 1997, Journal of clinical periodontology.

[126]  P. Adriaens,et al.  Effects of nonsurgical periodontal therapy on hard and soft tissues. , 2004, Periodontology 2000.

[127]  A. Braun,et al.  Removal of root substance with the Vector-system compared with conventional debridement in vitro. , 2005, Journal of clinical periodontology.

[128]  A. Braun,et al.  Subjective intensity of pain during the treatment of periodontal lesions with the Vector-system. , 2003, Journal of periodontal research.

[129]  S. Creanor,et al.  In vivo anticalculus effect of a dentifrice containing 0.5% zinc citrate trihydrate. , 1987, Caries research.

[130]  F. Grases,et al.  Phytate (Myo-inositol hexakisphosphate) inhibits cardiovascular calcifications in rats. , 2006, Frontiers in bioscience : a journal and virtual library.

[131]  R. Genco,et al.  Position paper: sonic and ultrasonic scalers in periodontics. Research, Science and Therapy Committee of the American Academy of Periodontology. , 2000, Journal of periodontology.

[132]  M. Nunn,et al.  The relationship between the presence of tooth-borne subgingival deposits and inflammation found with a dental endoscope. , 2008, Journal of periodontology.

[133]  G. Koch,et al.  Assessment of risk for periodontal disease. I. Risk indicators for attachment loss. , 1994, Journal of periodontology.

[134]  C. Dawes,et al.  A comparison of the site-specificity of supragingival and subgingival calculus deposition. , 1998, Journal of periodontology.

[135]  V. Segreto,et al.  Anticalculus effect of a dentifrice containing 0.5% zinc citrate trihydrate. , 1991, Community dentistry and oral epidemiology.

[136]  H. Löe,et al.  The natural history and clinical course of calculus formation in man. , 1991, Journal of clinical periodontology.

[137]  A. Aoki,et al.  The impact of laser application on periodontal and peri-implant wound healing. , 2009, Periodontology 2000.

[138]  N. Bissada,et al.  Efficiency of scaling of the molar furcation area with and without surgical access. , 1986, The International journal of periodontics & restorative dentistry.

[139]  J. Friskopp Ultrastructure of nondecalcified supragingival and subgingival calculus. , 1983, Journal of periodontology.

[140]  H. Shinoda,et al.  Pharmacological topics of bone metabolism: a novel bisphosphonate for the treatment of periodontitis. , 2008, Journal of pharmacological sciences.

[141]  D. A. Kerr,et al.  TISSUE RESPONSE IN THE GUINEA PIG TO STERILE AND NON-STERILE CALCULUS. , 1965, The Journal of periodontology.

[142]  H. Zander,et al.  Mineralization of Dental Calculus.∗ , 1960, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[143]  R Hickel,et al.  Working parameters of a magnetostrictive ultrasonic scaler influencing root substance removal in vitro. , 1998, Journal of periodontology.

[144]  C. Schwahn,et al.  Learning root debridement with curettes and power-driven instruments. Part I: a training program to increase effectivity. , 2002, Journal of clinical periodontology.

[145]  P. Schmidlin,et al.  Tooth substance loss resulting from mechanical, sonic and ultrasonic root instrumentation assessed by liquid scintillation. , 2001, Journal of clinical periodontology.

[146]  D. J. White,et al.  Anticalculus effects of a novel, dual-phase polypyrophosphate dentifrice: chemical basis, mechanism, and clinical response. , 2000, The journal of contemporary dental practice.

[147]  F. Abbas,et al.  Untreated periodontal disease in Indonesian adolescents. Longitudinal clinical data and prospective clinical and microbiological risk assessment. , 2000, Journal of clinical periodontology.

[148]  I. Mandel,et al.  Calculus revisited. A review. , 1986, Journal of clinical periodontology.

[149]  Ye Jin,et al.  Supragingival calculus: formation and control. , 2002, Critical reviews in oral biology and medicine : an official publication of the American Association of Oral Biologists.