Topography of glycosylation in the rough endoplasmic reticulum and Golgi apparatus.

PERSPECTIVES AND SUMMARy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . .. . . . . 64 TOPOGRAPHY OF ASPARAGINE GLYCOSYLATION IN THE ROUGH ENDOPLASMIC RETICULUM . . . . . . .. . .. . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . .. 64 Topography of Enzymes of GlclMan9GlcNAcrPP-Dolichol Synthesis and Transfer 65 Topography of Oligosaccharide-Lipid Intermediates in the Rough Endoplasmic Reticulum Membrane 67 Transport of Nucleotide Sugars into Rough Endoplasmic Reticulum Vesicles . . . . . . . . 69 Model for the Topography of Glycosylation in the Rough Endoplasmic Reticulum . .... . . . . . . . . . . . . . . . . . . . ..... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . 71 TOPOGRAPHY OF GLYCOSYLATION IN THE GOLGI APPARATUS . . . . . . . . . . . . . . . 7S Topography of Golgi Glycosyltransferases 75 Detection of Luminal Pools of Nucleotide Sugars . . . . . . . . . . . . ... . . . . . . . . . . . 75 Nucleotide Sugar Translocation into Golgi Vesicles 76 Physiological Relevance of Nucleotide Sugar Translocation into Golgi Vesicles . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79 Properties of Nucleotide Sugar and PAPS Translocation 80 Model foT' the Topography of Glycosylation and Sulfation in the Golgi Apparatus 83 FUTURE DIRECTIONS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ... . . . . . . . . . . . . . . . . . . . . . . . . . .. . .. . . . . . . . . . . . 84