Production of α-1,3-Galactosyltransferase Knockout Pigs by Nuclear Transfer Cloning

The presence of galactose α-1,3-galactose residues on the surface of pig cells is a major obstacle to successful xenotransplantation. Here, we report the production of four live pigs in which one allele of the α-1,3-galactosyltransferase locus has been knocked out. These pigs were produced by nuclear transfer technology; clonal fetal fibroblast cell lines were used as nuclear donors for embryos reconstructed with enucleated pig oocytes.

[1]  C. Polge,et al.  The effect of reducing the number of embryos during early stages of gestation on the maintenance of pregnancy in the pig. , 1966, Journal of reproduction and fertility.

[2]  H. R. Tervit,et al.  Production of cloned calves following nuclear transfer with cultured adult mural granulosa cells. , 1999, Biology of reproduction.

[3]  B. N. Day,et al.  Optimisation of porcine oocyte activation following nuclear transfer , 2000, Zygote.

[4]  F. A. P. León,et al.  Cloned transgenic calves produced from nonquiescent fetal fibroblasts. , 1998, Science.

[5]  Kwang-Wook Park,et al.  PRODUCTION OF NUCLEAR TRANSFER-DERIVED SWINE THAT EXPRESS THE ENHANCED GREEN FLUORESCENT PROTEIN , 2001, Animal biotechnology.

[6]  W. Fodor,et al.  Expression of the human α:1,2‐fucosyltransferase in transgenic pigs modifies the cell surface carbohydrate phenotype and confers resistance to human serum‐mediated cytolysis , 1999, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[7]  D. Sachs,et al.  Discordant organ xenotransplantation in primates: world experience and current status. , 1998, Transplantation.

[8]  T. Shigehisa,et al.  Remodeling of the Major Pig Xenoantigen by N-Acetylglucosaminyltransferase III in Transgenic Pig* , 2001, The Journal of Biological Chemistry.

[9]  S. Rosenberg,et al.  TRANSPLANTATION IN MINIATURE SWINE: I. Fixation of the Major Histocompatibility Complex , 1976, Transplantation.

[10]  W. F. Pope,et al.  Causes and consequences of early embryonic diversity in pigs. , 2020, Journal of reproduction and fertility. Supplement.

[11]  W. Fodor,et al.  Cloned pigs generated from cultured skin fibroblasts derived from a H‐transferase transgenic boar , 2001, Molecular reproduction and development.

[12]  P. Malý,et al.  Oocyte Galα1,3Gal Epitopes Implicated in Sperm Adhesion to the Zona Pellucida Glycoprotein ZP3 Are Not Required for Fertilization in the Mouse (*) , 1995, The Journal of Biological Chemistry.

[13]  A Onishi,et al.  Pig cloning by microinjection of fetal fibroblast nuclei. , 2000, Science.

[14]  Matthew W. Miller,et al.  Clinical and pathologic features of cloned transgenic calves and fetuses (13 case studies). , 1999, Theriogenology.

[15]  M. Pfister-Genskow,et al.  Production of cloned pigs from in vitro systems , 2000, Nature Biotechnology.

[16]  U. Galili.  Preformed and elicited antibodies to pig antigens in nonhuman primates and humans , 2001 .

[17]  A. Schnieke,et al.  Production of gene-targeted sheep by nuclear transfer from cultured somatic cells , 2000, Nature.

[18]  Yifan Dai,et al.  Cloned pigs produced by nuclear transfer from adult somatic cells , 2000, Nature.

[19]  J. Fletcher,et al.  Deletion of the α(1,3)galactosyl transferase (GGTA1) gene and the prion protein (PrP) gene in sheep , 2001, Nature Biotechnology.