In vitro IL-4, IL-12, and IFN-gamma production by splenocytes from Ancylostoma ceylanicum infected hamsters

The aim of the study was the production of chicken polyclonal antibodies recognising hamster IL-4, IL-12p35, and IFN- , which were further used to estimate cytokine concentrations in splenocyte culture supernatants, obtained from three age groups of Ancylostoma ceylanicum infected hamsters. Worm burdens differed significantly among age groups; the younger animals were the more heavily infected. Th1 and Th2 type cytokines were detected only in cultures containing spleen cells from the oldest hamsters, which were the most resistant to hookworm infection. In cultures of cells from young more susceptible hamsters, the presence of IL-4 and IL-12 but not IFN- were observed.

[1]  J. Behnke,et al.  The mucosal response to secondary infection with Ancylostoma ceylanicum in hamsters immunized by abbreviated primary infection , 2010, Parasite immunology.

[2]  C. Shoemaker,et al.  Mucosal antibody responses in experimental hookworm infection , 2008, Parasite immunology.

[3]  S. Brooker,et al.  Stage-specific immune responses in human Necator americanus infection , 2007, Parasite immunology.

[4]  A. Loukas,et al.  Reduction of Worm Fecundity and Canine Host Blood Loss Mediates Protection against Hookworm Infection Elicited by Vaccination with Recombinant Ac-16 , 2007, Clinical and Vaccine Immunology.

[5]  V. Rutten,et al.  Cloning, sequencing and expression of white rhinoceros (Ceratotherium simum) interferon-gamma (IFN-γ) and the production of rhinoceros IFN-γ specific antibodies , 2007 .

[6]  R. Fujiwara,et al.  Comparative immunology of human and animal models of hookworm infection , 2006, Parasite immunology.

[7]  Peter J Hotez,et al.  Soil-transmitted helminth infections: ascariasis, trichuriasis, and hookworm , 2006, The Lancet.

[8]  P. Hotez,et al.  The impact of concurrent and treated Ancylostoma ceylanicum hookworm infections on the immunogenicity of a recombinant hookworm vaccine in hamsters. , 2006, The Journal of infectious diseases.

[9]  A. Loukas,et al.  Vaccination with Recombinant Aspartic Hemoglobinase Reduces Parasite Load and Blood Loss after Hookworm Infection in Dogs , 2005, PLoS medicine.

[10]  H. Gram,et al.  Production of antibodies to canine IL-1beta and canine TNF to assess the role of proinflammatory cytokines. , 2005, Veterinary immunology and immunopathology.

[11]  P. Hotez,et al.  Host Cytokine Production, Lymphoproliferation, and Antibody Responses during the Course of Ancylostoma ceylanicum Infection in the Golden Syrian Hamster , 2005, Infection and Immunity.

[12]  E. Długosz.  Hookworm infections in human and laboratory animals--differences and similarities in immune responses. , 2005, Wiadomosci parazytologiczne.

[13]  D. Pritchard,et al.  Immune responses in human necatoriasis: association between interleukin-5 responses and resistance to reinfection. , 2004, The Journal of infectious diseases.

[14]  R. Corrêa-Oliveira,et al.  Cellular responses and cytokine production in post‐treatment hookworm patients from an endemic area in Brazil , 2004, Clinical and experimental immunology.

[15]  E. Graham,et al.  Development of antibodies to feline IFN-γ as tools to elucidate the cellular immune responses to FeLV , 2003, Journal of Immunological Methods.

[16]  Rodrigo Corrêa-Oliveira,et al.  Cellular responses and cytokine profiles in Ascaris lumbricoides and Trichuris trichiura infected patients , 2002, Parasite immunology.

[17]  A. Loukas,et al.  Immune Responses in Hookworm Infections , 2001, Clinical Microbiology Reviews.

[18]  R. Corrêa-Oliveira,et al.  Natural versus drug-induced resistance in Schistosoma mansoni infection. , 2000, Parasitology today.

[19]  P. Enyong,et al.  Immunity to Onchocerciasis: Cells from Putatively Immune Individuals Produce Enhanced Levels of Interleukin-5, Gamma Interferon, and Granulocyte-Macrophage Colony-Stimulating Factor in Response to Onchocerca volvulus Larval and Male Worm Antigens , 2000, Infection and Immunity.

[20]  P. Hotez,et al.  Epidemiology of hookworm infection in Itagua, Paraguay: a cross sectional study. , 1999, Memorias do Instituto Oswaldo Cruz.

[21]  J. V. van Dongen,et al.  Analysing the developing lymphocyte system of neonates and infants , 1999, European Journal of Pediatrics.

[22]  J. Behnke,et al.  Expression of acquired immunity to the hookworm Ancylostoma ceylanicum in hamsters , 1997, Parasite immunology.

[23]  H. Wędrychowicz,et al.  Host antibody recognition of surface and somatic antigens of the parasitic developmental stages of Ancylostoma ceylanicum , 1996 .

[24]  T. Macdonald,et al.  Intestinal nematode infections in children: the pathophysiological price paid , 1992, Parasitology.

[25]  D. Lewis,et al.  Cellular and molecular mechanisms for reduced interleukin 4 and interferon-gamma production by neonatal T cells. , 1991, The Journal of clinical investigation.

[26]  J. Behnke,et al.  Acquired immunity to Ancylostoma ceylanicum in hamsters , 1990, Parasite immunology.

[27]  J. Behnke,et al.  Ancylostoma ceylanicum in the hamster: observations on the host—parasite relationship during primary infection , 1989, Parasitology.

[28]  A. Fleming.  Iron-deficiency in the tropics. , 1977, Scandinavian journal of haematology. Supplementum.

[29]  T. Miller.  Influence of age and sex on susceptibility of dogs to primary infection with Ancylostoma caninum. , 1965, The Journal of parasitology.