Self-referencing SPR-biosensors based on penetration difference of evanescent waves.

SPR based biosensors register binding of analytes to the surface with immobilized receptors by measuring changes of the refractive index near this surface. An important task in the improvement of this measurement technology is a separation of signals, corresponding to the changes in the chemosensitive layer, from undesired contributions of bulk phase, for example, due to fluctuations of temperature, concentrations of solutes, pressure. The wavelength of the incident light influences strongly the penetration depth of the corresponding evanescent wave. This dependence was exploited here for compensation of the contribution of the bulk refractive index. It was performed using differential SPR measurements at two wavelengths with differing penetration depths. Theoretical analysis and numerical optimization of the suggested approach, named a Penetration Difference Self-Referencing SPR (PDSR-SPR), were performed. Experimental test was performed using 658 and 980 nm laser diodes. Over 20 times suppression of variations of bulk refractive index with magnitude up to 1000 μRIU was observed. Finally, PDSR-SPR approach was applied for monitoring of antibodies binding to the immobilized antigens.

[1]  Wolfgang Knoll,et al.  Immunosensor with self-referencing based on surface plasmon diffraction. , 2004, Analytical chemistry.

[2]  J H Grassi,et al.  Temperature-Dependent Refractive Index Determination from Critical Angle Measurements:  Implications for Quantitative SPR Sensing. , 1999, Analytical chemistry.

[3]  W. Wagner,et al.  The IAPWS Formulation 1995 for the Thermodynamic Properties of Ordinary Water Substance for General and Scientific Use , 2002 .

[4]  Jing Guo,et al.  Dual-mode surface-plasmon-resonance sensors using angular interrogation. , 2008 .

[5]  J. Xiang,et al.  Electrochemical Surface Plasmon Resonance Spectroscopy at Bilayered Silver/Gold Films. , 2007, The journal of physical chemistry. C, Nanomaterials and interfaces.

[6]  C. Grunwald,et al.  Noise reduction by multiple referencing in surface plasmon resonance imaging. , 2008, The Review of scientific instruments.

[7]  B. Liedberg,et al.  Surface plasmon resonance for gas detection and biosensing , 1983 .

[8]  B. Liedberg,et al.  Principles of biosensing with an extended coupling matrix and surface plasmon resonance , 1993 .

[9]  Nongjian Tao,et al.  High-performance differential surface plasmon resonance sensor using quadrant cell photodetector , 2003 .

[10]  R. Georgiadis,et al.  Two-color approach for determination of thickness and dielectric constant of thin films using surface plasmon resonance spectroscopy , 1996 .

[11]  K. A. Peterlinz,et al.  Comment on “Characterization of Self-Assembled Monolayers on Silver and Gold Using Surface Plasmon Resonance Spectroscopy” , 1997 .

[12]  C. Thirstrup,et al.  Temperature-dependent sensitivity of surface plasmon resonance sensors at the gold–water interface , 2008 .

[13]  Ingemar Lundström,et al.  Bioanalysis with surface plasmon resonance , 1991 .

[14]  J. Homola.  Present and future of surface plasmon resonance biosensors , 2003, Analytical and bioanalytical chemistry.

[15]  B. Liedberg,et al.  Biosensing with surface plasmon resonance--how it all started. , 1995, Biosensors & bioelectronics.

[16]  J. Homola.  Surface plasmon resonance based sensors , 2006 .

[17]  R. Robelek.  Surface plasmon resonance sensors in cell biology: basics and application , 2009 .

[18]  Radan Slavik,et al.  Advanced biosensing using simultaneous excitation of short and long range surface plasmons , 2006 .

[19]  Nobuko I. Wakayama,et al.  Measurement of temperature and concentration dependences of refractive index of hen‐egg‐white lysozyme solution , 2003 .

[20]  L. Noe,et al.  Characterization of Self-Assembled Alkanethiol Monolayers on Silver and Gold Using Surface Plasmon Spectroscopy , 1997 .

[21]  R. Schasfoort,et al.  Handbook of surface plasmon resonance , 2008 .

[22]  Erik Wischerhoff,et al.  Real-Time Two-Wavelength Surface Plasmon Resonance as a Tool for the Vertical Resolution of Binding Processes in Biosensing Hydrogels , 2002 .

[23]  L G Bachas,et al.  Optimal self-referenced sensing using long- and short- range surface plasmons. , 2007, Optics express.

[24]  Yuri M. Shirshov,et al.  Bimetallic Layers Increase Sensitivity of Affinity Sensors Based on Surface Plasmon Resonance , 2002 .

[25]  W. Worek,et al.  Two-wavelength interferometric technique for measuring the refractive index of salt-water solutions. , 1993, Applied optics.

[26]  I. Nikolov,et al.  Analysis of the dispersion of optical plastic materials , 2007 .

[27]  Sinclair S. Yee,et al.  Quantifying the information content of surface plasmon resonance reflection spectra , 1998 .

[28]  Christian Grunwald,et al.  Double-wavelength technique for surface plasmon resonance measurements: basic concept and applications for single sensors and two-dimensional sensor arrays. , 2005, Analytical chemistry.

[29]  J. Homola,et al.  Surface plasmon resonance (SPR) sensors: approaching their limits? , 2009, Optics express.

[30]  Allan H. Harvey,et al.  Revised Formulation for the Refractive Index of Water and Steam as a Function of Wavelength, Temperature and Density , 1998 .