Hematopoietic Progenitor Cell Rolling in Bone Marrow Microvessels: Parallel Contributions by Endothelial Selectins and Vascular Cell Adhesion Molecule 1

We have used intravital microscopy to study physiologically perfused microvessels in murine bone marrow (BM). BM sinusoids and venules, but not adjacent bone vessels, supported rolling interactions of hematopoietic progenitor cells. Rolling did not involve L-selectin, but was partially reduced in wild-type mice treated with antibodies to P- or E-selectin and in mice that were deficient in these two selectins. Selectin-independent rolling was mediated by α4 integrins, which interacted with endothelial vascular cell adhesion molecule (VCAM)-1. Parallel contribution of the endothelial selectins and VCAM-1 is not known to direct blood cell trafficking to other noninflamed tissues. This combination of constitutively expressed adhesion molecules may thus constitute a BM-specific recruitment pathway for progenitor cells analogous to the vascular addressins that direct selective lymphocyte homing to lymphoid organs.

[1]  A. Beaudet,et al.  The Association between α4-Integrin, P-Selectin, and E-Selectin in an Allergic Model of Inflammation , 1997, The Journal of experimental medicine.

[2]  R. Hynes.  Targeted mutations in cell adhesion genes: what have we learned from them? , 1996, Developmental biology.

[3]  E. Dzierzak,et al.  Characterization of the first definitive hematopoietic stem cells in the AGM and liver of the mouse embryo. , 1996, Immunity.

[4]  M. Cybulsky,et al.  Vascular cell adhesion molecule‐1 expression by hematopoiesis‐supporting stromal cells is not essential for lymphoid or myeloid differentiation in vivo or in vitro , 1996, European journal of immunology.

[5]  G S Kansas,et al.  Selectins and their ligands: current concepts and controversies. , 1996, Blood.

[6]  P. Kubes,et al.  Leukocyte-endothelial cell interactions evoked by mast cells. , 1996, Cardiovascular research.

[7]  Petr Malý,et al.  The α(1,3)Fucosyltransferase Fuc-TVII Controls Leukocyte Trafficking through an Essential Role in L-, E-, and P-selectin Ligand Biosynthesis , 1996, Cell.

[8]  R. Hynes,et al.  Differential Requirements for α4 Integrins during Fetal and Adult Hematopoiesis , 1996, Cell.

[9]  P. Kubes,et al.  The alpha 4-integrin supports leukocyte rolling and adhesion in chronically inflamed postcapillary venules in vivo , 1996, The Journal of experimental medicine.

[10]  L. Picker,et al.  Lymphocyte Homing and Homeostasis , 1996, Science.

[11]  Richard O Hynes,et al.  Susceptibility to Infection and Altered Hematopoiesis in Mice Deficient in Both P- and E-Selectins , 1996, Cell.

[12]  J F Keij,et al.  Homing of fluorescently labeled murine hematopoietic stem cells. , 1996, Experimental hematology.

[13]  C. E. van der Schoot,et al.  Constitutive expression of E-selectin and vascular cell adhesion molecule-1 on endothelial cells of hematopoietic tissues. , 1996, The American journal of pathology.

[14]  Paul,et al.  Adhesion receptors on bone marrow stromal cells: in vivo expression of vascular cell adhesion molecule-1 by reticular cells and sinusoidal endothelium in normal and gamma-irradiated mice. , 1996, Blood.

[15]  U. V. von Andrian.  Intravital microscopy of the peripheral lymph node microcirculation in mice. , 1996, Microcirculation.

[16]  D. G. Osmond,et al.  Adhesion receptors on bone marrow stromal cells: in vivo expression of vascular cell adhesion molecule-1 by reticular cells and sinusoidal endothelium in normal and gamma-irradiated mice , 1996 .

[17]  Mark V. Dahl,et al.  α4 Integrins mediate lymphocyte attachment and rolling under physiologic flow , 1996 .

[18]  A. Cumano,et al.  Circulation of hematopoietic progenitors in the mouse embryo. , 1996, Immunity.

[19]  C. Craddock,et al.  The VLA4/VCAM-1 adhesion pathway defines contrasting mechanisms of lodgement of transplanted murine hemopoietic progenitors between bone marrow and spleen. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[20]  A. Zannettino,et al.  Primitive human hematopoietic progenitors adhere to P-selectin (CD62P). , 1995, Blood.

[21]  S. Rodenhuis,et al.  Expression of adhesion molecules on CD34+ cells: CD34+ L-selectin+ cells predict a rapid platelet recovery after peripheral blood stem cell transplantation. , 1995, Blood.

[22]  M. Vadas,et al.  Cytokines increase human hemopoietic cell adhesiveness by activation of very late antigen (VLA)-4 and VLA-5 integrins , 1995, The Journal of experimental medicine.

[23]  T. Springer,et al.  The integrin VLA-4 supports tethering and rolling in flow on VCAM-1 , 1995, The Journal of cell biology.

[24]  E. Berg,et al.  α4 integrins mediate lymphocyte attachment and rolling under physiologic flow , 1995, Cell.

[25]  A. Beaudet,et al.  Sequential contribution of L- and P-selectin to leukocyte rolling in vivo , 1995, The Journal of experimental medicine.

[26]  D. Scadden,et al.  Functional isolation and characterization of human hematopoietic stem cells. , 1995, Science.

[27]  C. Craddock,et al.  VLA4/VCAM‐1接着経路が骨髄とひ臓の間で移植したマウス造血幹細胞の対照的な定着機構を決定する , 1995 .

[28]  L. McIntire,et al.  A two-step adhesion cascade for T cell/endothelial cell interactions under flow conditions. , 1994, The Journal of clinical investigation.

[29]  D. Benayahu,et al.  Myeloblastic cell line expresses osteoclastic properties following coculture with marrow stromal adipocytes , 1994, Journal of cellular biochemistry.

[30]  E. Butcher,et al.  L-selectin and very late antigen-4 integrin promote eosinophil rolling at physiological shear rates in vivo. , 1994, Journal of immunology.

[31]  K. Ley,et al.  Lymphocyte homing and leukocyte rolling and migration are impaired in L-selectin-deficient mice. , 1994, Immunity.

[32]  G S Kansas,et al.  Monocyte rolling, arrest and spreading on IL-4-activated vascular endothelium under flow is mediated via sequential action of L-selectin, beta 1-integrins, and beta 2-integrins , 1994, The Journal of cell biology.

[33]  T. Springer.  Traffic signals for lymphocyte recirculation and leukocyte emigration: The multistep paradigm , 1994, Cell.

[34]  B. Furie,et al.  Expression cloning of a functional glycoprotein ligand for P-selectin , 1993, Cell.

[35]  T. Papayannopoulou,et al.  Peripheralization of hemopoietic progenitors in primates treated with anti-VLA4 integrin. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[36]  R. Mulligan,et al.  The basic science of gene therapy. , 1993, Science.

[37]  E. Zanjani,et al.  Liver-derived fetal hematopoietic stem cells selectively and preferentially home to the fetal bone marrow , 1993 .

[38]  E. Butcher.  Leukocyte-endothelial cell recognition: Three (or more) steps to specificity and diversity , 1991, Cell.

[39]  K. Ley,et al.  Endothelial, not hemodynamic, differences are responsible for preferential leukocyte rolling in rat mesenteric venules. , 1991, Circulation research.

[40]  E. Berg,et al.  A carbohydrate domain common to both sialyl Le(a) and sialyl Le(X) is recognized by the endothelial cell leukocyte adhesion molecule ELAM-1. , 1991, The Journal of biological chemistry.

[41]  K. Ley,et al.  Rapid leukocyte accumulation by "spontaneous" rolling and adhesion in the exteriorized rabbit mesentery. , 1991, International journal of microcirculation, clinical and experimental.

[42]  M. Tavassoli,et al.  Molecular basis of homing of intravenously transplanted stem cells to the marrow. , 1990, Blood.

[43]  M. Belvin,et al.  Granulocyte-macrophage colony-stimulating factor and other cytokines regulate surface expression of the leukocyte adhesion molecule-1 on human neutrophils, monocytes, and their precursors. , 1990, Journal of immunology.

[44]  B. Seed,et al.  Endothelial leukocyte adhesion molecule 1: an inducible receptor for neutrophils related to complement regulatory proteins and lectins. , 1989, Science.

[45]  Pries Ar,et al.  A versatile video image analysis system for microcirculatory research. , 1988 .

[46]  A. Pries,et al.  A versatile video image analysis system for microcirculatory research. , 1988, International journal of microcirculation, clinical and experimental.

[47]  T. Dexter,et al.  Self-renewal and differentiation of interleukin-3-dependent multipotent stem cells are modulated by stromal cells and serum factors. , 1986, Differentiation; research in biological diversity.

[48]  I. Bertoncello,et al.  Multiparameter analysis of transplantable hemopoietic stem cells: I. The separation and enrichment of stem cells homing to marrow and spleen on the basis of rhodamine-123 fluorescence. , 1985, Experimental hematology.

[49]  M L Walsh,et al.  Monitoring of relative mitochondrial membrane potential in living cells by fluorescence microscopy , 1981, The Journal of cell biology.

[50]  S. Michelson,et al.  The migration of blood cells of the bone marrow through the sinusoidal wall , 1971, Journal of morphology.

[51]  P. Branemark.  Vital microscopy of bone marrow in rabbit. , 1959, Scandinavian journal of clinical and laboratory investigation.