Population Structure and Cryptic Evolutionary Units in the Alligator Snapping Turtle

The alligator snapping turtle ( Macroclemys temminckii) is a long‐lived, slow‐growing chelydrid turtle found in Gulf of Mexico drainages from Florida to Texas (U.S.A.). Populations are thought to be depleted throughout the range due in part to an increased harvest in the 1960s through 1980s. To identify population and evolutionary units, 420 base pairs were sequenced within the mitochondrial DNA control region of 158 specimens from 12 drainages. Results indicate substantial phylogeographic structuring and strong population‐level separations among river drainages. Eight of 11 haplotypes were observed to be river‐specific, providing diagnostic markers for most drainages. Three partitions are resolved in the mtDNA genealogy, corresponding to the eastern, central, and western portion of the species’ range. These separations coincide with recognized biogeographic provinces. The population structure by river system indicates that many drainages are distinct management units, with the Suwannee River lineage possibly deserving special attention, based on the criterion of genetic distinctiveness. The partitioning of M. temminckii into river‐specific populations illustrates the management framework and conservation challenges that apply to a broad array of riverine species. Drainage‐specific molecular markers may be used to identify the geographic origin of turtle products in the marketplace.

[1]  J. Avise,et al.  PRINCIPLES OF PHYLOGEOGRAPHY AS ILLUSTRATED BY FRESHWATER AND TERRESTRIAL TURTLES IN THE SOUTHEASTERN UNITED STATES , 1998 .

[2]  P. Moler,et al.  Phylogeographic uniformity in mitochondrial DNA of the snapping turtle (Chelydra serpentina) , 1998 .

[3]  F. Cipriano,et al.  Molecular genetic identification of whale and dolphin products from commercial markets in Korea and Japan , 1996 .

[4]  M. Miyamoto,et al.  Phylogeography and population structure of the Atlantic and Mediterranean green turtle Chelonia mydas: a mitochondrial DNA control region sequence assessment , 1996, Molecular ecology.

[5]  W. W. Dimmick,et al.  Conservation Genetics of the Common Snapping Turtle (Chelydra serpentina) , 1996 .

[6]  D. Hillis.  Nucleic acids IV : Sequencing and cloning , 1996 .

[7]  C. M. Allen,et al.  MOVEMENTS AND HABITAT USE OF SUBADULT ALLIGATOR SNAPPING TURTLES (MACROCLEMYS TEMMINCKII) IN LOUISIANA , 1996 .

[8]  C. Lydeard,et al.  A Diverse and Endangered Aquatic Ecosystem of the Southeast United States , 1995 .

[9]  I. Barák,et al.  A comparison of mtDNA restriction sites vs. control region sequences in phylogeographic assessment of the musk turtle (Sternotherus minor) , 1995, Molecular ecology.

[10]  C. Lydeard,et al.  Molecular Systematics of Map Turtles (Graptemys): A Comparison of Mitochondrial Restriction Site Versus Sequence Data , 1994 .

[11]  C. Nilsson,et al.  Fragmentation and Flow Regulation of River Systems in the Northern Third of the World , 1994, Science.

[12]  C. Moritz,et al.  Sequence evolution and phylogenetic signal in control-region and cytochrome b sequences of rainbow fishes (Melanotaeniidae). , 1994, Molecular biology and evolution.

[13]  A. Baker,et al.  Patterns of sequence variation in the mitochondrial D-loop region of shrews. , 1994, Molecular biology and evolution.

[14]  A. Pudovkin,et al.  Two Programs to Estimate Significance of χ2 Values Using Pseudo-Probability Tests , 1993 .

[15]  A. Hoelzel.  Molecular genetic analysis of populations: a practical approach. , 1993 .

[16]  T. W. Quinn.  The genetic legacy of Mother Goose– phylogeographic patterns of lesser snow goose Chen caerulescens caerulescens maternal lineages , 1992, Molecular ecology.

[17]  L. Excoffier,et al.  Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. , 1992, Genetics.

[18]  J. Avise.  Molecular population structure and the biogeographic history of a regional fauna : a case history with lessons for conservation biology , 1992 .

[19]  P. S. White,et al.  Mitochondrial DNA isolation. , 1992 .

[20]  P. Pritchard.  The Alligator Snapping Turtle: Biology and Conservation , 1989 .

[21]  M. Slatkin,et al.  A COMPARISON OF THREE INDIRECT METHODS FOR ESTIMATING AVERAGE LEVELS OF GENE FLOW , 1989, Evolution; international journal of organic evolution.

[22]  J. Avise.  A role for molecular genetics in the recognition and conservation of endangered species. , 1989, Trends in ecology & evolution.

[23]  D. Roff,et al.  The statistical analysis of mitochondrial DNA polymorphisms: chi 2 and the problem of small samples. , 1989, Molecular biology and evolution.

[24]  R. Mayden.  VICARIANCE BIOGEOGRAPHY, PARSIMONY, AND EVOLUTION IN NORTH AMERICAN FRESHWATER FISHES , 1988 .

[25]  N. Saitou,et al.  The neighbor-joining method: a new method for reconstructing phylogenetic trees. , 1987, Molecular biology and evolution.

[26]  M. Nei.  Molecular Evolutionary Genetics , 1987 .

[27]  E. Bermingham,et al.  Molecular zoogeography of freshwater fishes in the southeastern United States. , 1986, Genetics.

[28]  M. Slatkin.  RARE ALLELES AS INDICATORS OF GENE FLOW , 1985, Evolution; international journal of organic evolution.

[29]  P. Pritchard.  Encyclopedia of turtles , 1979 .

[30]  J. T. Collins,et al.  A Field Guide to Reptiles and Amphibians: Eastern and Central North America , 1975 .

[31]  Clifford H. Pope,et al.  Turtles of the United States and Canada , 1939 .