Concentration of Individual Red Cells by Laser Light Scattering

Cell volume (MCV) and hemoglobin concentration (MCHC) are the red cell indices used to characterize the blood of patients with anemia. Since the introduction of flow cytometric methods for the measurement of these indices, it has generally been assumed that the values derived by these instruments are accurate. However. it has recently been shown that a number of cellular factors. including alterations in cellular deformability. can lead to inaccurate measurement of cell volume by these automated instruments. Because cell hemoglobin concentration and hematocrit are computed from the measured values of cell volume. accuracy of these indices is also compromised by inaccurate determination of cell volume. A recently developed experimental flow cytometric method based on laser light scattering. which can independently measure volume and hemoglobin concentration. has been used in the present study to measure MCV and MCHC of density-fractionated normal and sickle red cells. hydrated and dehydrated

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