Time-resolved fluorescence spectroscopy of human brain tumors

Fluorescence spectroscopy of the endogenous emission of brain tumors has been researched as a potentially important method for the intraoperative localization of brain tumor margins. In this study, we investigate the use of time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) for demarcation of primary brain tumors by studying the time-resolved spectra of gliomas of different histologic grades. Time-resolved fluorescence (3 ns, 337 nm excitation) from excised human brain tumor show differences between the time-resolved emission of malignant glioma and normal brain tissue (gray and white matter). Our findings suggest that brain tumors can be differentiated from normal brain tissue based upon unique time-resolved fluorescence signature.

[1]  Robert R. Alfano,et al.  Time-resolved fluorescence of benign and malignant breast tissues , 1992 .

[2]  G. Bottiroli,et al.  Brain tissue autofluorescence: an aid for intraoperative delineation of tumor resection margins. , 1998, Cancer detection and prevention.

[3]  J. Lakowicz.  Principles of fluorescence spectroscopy , 1983 .

[4]  R R Alfano,et al.  Fluorescence polarization spectroscopy and time-resolved fluorescence kinetics of native cancerous and normal rat kidney tissues. , 1986, Biophysical journal.

[5]  Laura Marcu,et al.  Photobleaching of Arterial Fluorescent Compounds: Characterization of Elastin, Collagen and Cholesterol Time‐resolved Spectra during Prolonged Ultraviolet Irradiation , 1999, Photochemistry and photobiology.

[6]  Robert R. Alfano,et al.  Steady state and time-resolved laser fluorescence from normal and tumor lung and breast tissues , 1988 .

[7]  S. L. Jacques,et al.  Diagnostic potential of laser-induced autofluorescence emission in brain tissue. , 1997, Journal of Korean medical science.

[8]  M Motamedi,et al.  Brain tumor demarcation using optical spectroscopy; an in vitro study. , 2000, Journal of biomedical optics.

[9]  B. Wilson,et al.  In Vivo Fluorescence Spectroscopy and Imaging for Oncological Applications , 1998, Photochemistry and photobiology.

[10]  E. Sevick-Muraca,et al.  Quantitative optical spectroscopy for tissue diagnosis. , 1996, Annual review of physical chemistry.

[11]  Robert R. Alfano,et al.  Time-resolved UV photoexcited fluorescence kinetics from malignant and non-malignant human breast tissues , 1992 .

[12]  Kenneth R. Hess,et al.  Extent of Resection as a Prognostic Variable in the Treatment of Gliomas , 1999, Journal of Neuro-Oncology.

[13]  Anita Mahadevan-Jansen,et al.  In Vivo Brain Tumor Demarcation Using Optical Spectroscopy¶ , 2001, Photochemistry and photobiology.

[14]  Stefan Andersson-Engels,et al.  The use of time-resolved fluorescence for diagnosis of atherosclerotic plaque and malignant tumours , 1990 .

[15]  Gene H. Barnett,et al.  The Role of Image-guided Technology in the Surgical Planning and Resection of Gliomas , 1999, Journal of Neuro-Oncology.