Live Cell Analysis Under Shear Flow

This chapter describes a microfluidic system for live cell analysis that enables precise control over shear stress to emulate physiological conditions encountered in the vasculature and other niches. Data from a number of key application areas are included in the report, including stem cell biology, platelet biology, immune response, and bacterial biofilms. The approach addresses critical limitations of macroscopic approaches like laminar flow chambers or cone and plate assays by providing real-time, high-resolution microscopy datain a low-volume microfluidic format. Utilizing a pneumatic pumping system instead of liquid displacement pumps obviates the need to sterilize components between runs and contributes to higher throughput and parallelization. The microfluidic device is contained within an SBS-standard well plate format that remedies many of the interfacing issues often encountered with hand-made microfluidic devices.

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