Cellulosome assembly: paradigms are meant to be broken!

Cohesin-Dockerin interactions are at the core of cellulosomal assembly and organization. They are highly specific and form stable complexes, allowing cellulosomes to adopt distinct conformations. Each cellulosomal system seems to have a particular organizational strategy that can vary in complexity according to the nature of its Cohesin-Dockerin interactions. Hence, several efforts have been undertaken to reveal the mechanisms that govern the specificity, affinity and flexibility of these protein-protein interactions. Here we review the most recent studies that have focused on the structural aspects of Cohesin-Dockerin recognition. They reveal an ever-increasing number of subtle intricacies suggesting that cellulosome assembly is more complex than was initially thought.

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[2]  E. Bayer,et al.  Single Binding Mode Integration of Hemicellulose-degrading Enzymes via Adaptor Scaffoldins in Ruminococcus flavefaciens Cellulosome* , 2016, The Journal of Biological Chemistry.

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[9]  Harry J. Gilbert,et al.  Novel Clostridium thermocellum Type I Cohesin-Dockerin Complexes Reveal a Single Binding Mode* , 2012, The Journal of Biological Chemistry.

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[12]  Pedro Bule,et al.  Assembly of Ruminococcus flavefaciens cellulosome revealed by structures of two cohesin-dockerin complexes , 2017, Scientific Reports.

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[14]  B. Henrissat,et al.  Broad phylogeny and functionality of cellulosomal components in the bovine rumen microbiome , 2016, Environmental microbiology.

[15]  H. Fierobe,et al.  Sequence Analysis of Scaffolding Protein CipC and ORFXp, a New Cohesin-Containing Protein inClostridium cellulolyticum: Comparison of Various Cohesin Domains and Subcellular Localization of ORFXp , 1999, Journal of bacteriology.

[16]  Daniel B. Fried,et al.  Resolving dual binding conformations of cellulosome cohesin-dockerin complexes using single-molecule force spectroscopy , 2015, eLife.

[17]  E. Bayer,et al.  Standalone cohesin as a molecular shuttle in cellulosome assembly , 2015, FEBS letters.

[18]  Ora Schueler-Furman,et al.  Crucial Roles of Single Residues in Binding Affinity, Specificity, and Promiscuity in the Cellulosomal Cohesin-Dockerin Interface* , 2015, The Journal of Biological Chemistry.

[19]  Conrad C. Huang,et al.  UCSF Chimera—A visualization system for exploratory research and analysis , 2004, J. Comput. Chem..

[20]  Michael A Nash,et al.  Single versus dual-binding conformations in cellulosomal cohesin-dockerin complexes. , 2016, Current opinion in structural biology.

[21]  Anders F. Andersson,et al.  Ninety-nine de novo assembled genomes from the moose (Alces alces) rumen microbiome provide new insights into microbial plant biomass degradation , 2017, The ISME Journal.

[22]  E. Bayer,et al.  Cohesin‐dockerin microarray: Diverse specificities between two complementary families of interacting protein modules , 2008, Proteomics.

[23]  Harry J. Gilbert,et al.  Cellulosome assembly revealed by the crystal structure of the cohesin–dockerin complex , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[24]  E. Bayer,et al.  Combined Crystal Structure of a Type I Cohesin , 2015, The Journal of Biological Chemistry.

[25]  E. Bayer,et al.  Lysozyme activity of the Ruminococcus champanellensis cellulosome. , 2016, Environmental microbiology.

[26]  Raphael Lamed,et al.  A Novel Acetivibrio cellulolyticus Anchoring Scaffoldin That Bears Divergent Cohesins , 2004, Journal of bacteriology.

[27]  Daniel B. Fried,et al.  Insights into a type III cohesin–dockerin recognition interface from the cellulose‐degrading bacterium Ruminococcus flavefaciens , 2015, Journal of molecular recognition : JMR.

[28]  Structure–function analyses generate novel specificities to assemble the components of multienzyme bacterial cellulosome complexes , 2018 .

[29]  B. White,et al.  Ruminococcal cellulosome systems from rumen to human. , 2015, Environmental microbiology.

[30]  Sagar M. Utturkar,et al.  Near-Complete Genome Sequence of the Cellulolytic Bacterium Bacteroides (Pseudobacteroides) cellulosolvens ATCC 35603 , 2015, Genome Announcements.

[31]  Pedro Bule,et al.  Cell-surface Attachment of Bacterial Multienzyme Complexes Involves Highly Dynamic Protein-Protein Anchors* , 2015, The Journal of Biological Chemistry.

[32]  E. Bayer,et al.  Cellulosome: a discrete cell surface organelle of Clostridium thermocellum which exhibits separate antigenic, cellulose-binding and various cellulolytic activities , 1983 .

[33]  E. Bayer,et al.  Diverse specificity of cellulosome attachment to the bacterial cell surface , 2016, Scientific Reports.

[34]  Marek Cieplak,et al.  Large conformational fluctuations of the multi-domain xylanase Z of Clostridium thermocellum. , 2015, Journal of structural biology.

[35]  Raphael Lamed,et al.  ScaC, an Adaptor Protein Carrying a Novel Cohesin That Expands the Dockerin-Binding Repertoire of the Ruminococcus flavefaciens 17 Cellulosome , 2004, Journal of bacteriology.

[36]  E. Bayer,et al.  Unconventional Mode of Attachment of the Ruminococcus flavefaciens Cellulosome to the Cell Surface , 2005, Journal of bacteriology.

[37]  Sagar M. Utturkar,et al.  Unique organization and unprecedented diversity of the Bacteroides (Pseudobacteroides) cellulosolvens cellulosome system , 2017, Biotechnology for Biofuels.

[38]  E. Bayer,et al.  Cellulosomics of the cellulolytic thermophile Clostridium clariflavum , 2014, Biotechnology for Biofuels.

[39]  B. White,et al.  Atypical Cohesin-Dockerin Complex Responsible for Cell Surface Attachment of Cellulosomal Components , 2013, The Journal of Biological Chemistry.

[40]  Alvaro G. Hernandez,et al.  Diversity and Strain Specificity of Plant Cell Wall Degrading Enzymes Revealed by the Draft Genome of Ruminococcus flavefaciens FD-1 , 2009, PloS one.

[41]  B. Różycki,et al.  The length but not the sequence of peptide linker modules exerts the primary influence on the conformations of protein domains in cellulosome multi-enzyme complexes. , 2017, Physical chemistry chemical physics : PCCP.

[42]  Z. Jia,et al.  Mechanism of bacterial cell-surface attachment revealed by the structure of cellulosomal type II cohesin-dockerin complex. , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[43]  Edward A Bayer,et al.  Evidence for a dual binding mode of dockerin modules to cohesins , 2007, Proceedings of the National Academy of Sciences.

[44]  E. Bayer,et al.  Enzymatic profiling of cellulosomal enzymes from the human gut bacterium, Ruminococcus champanellensis, reveals a fine-tuned system for cohesin-dockerin recognition. , 2016, Environmental microbiology.