论文信息 - Apoptosis in thyroid tissue from patients with Hashimoto's thyroiditis.

Apoptosis in thyroid tissue from patients with Hashimoto's thyroiditis.

To clarify whether apoptosis of thyroid follicular epithelial cells occurs at the tissue level in autoimmune thyroiditis, 17 specimens of thyroid tissues with Hashimoto's thyroiditis were stained for fragmented DNA. Almost all nuclei of follicular epithelial cells forming atrophic thyroid follicles surrounded by mononuclear cell infiltration and fibrosis showed positive staining. With increasing distance from lymphoid cell follicles, the percentage of follicular epithelial cells with DNA fragmentation-positive nuclei decreased (30-80%). Electron microscopic study revealed the existence of epithelial cells with shrunk and condensed nuclei. The frequency of those cells in different areas was almost compatible with that of cells with fragmentation-positive nuclei. These findings suggest that apoptosis plays an important role in the thyroid tissue injury in autoimmune thyroiditis.

[1] G. Kroemer,et al. Pharmacological inhibition of programmed lymphocyte death. , 1994, Immunology today.

[2] P. Henkart. Mechanism of lymphocyte-mediated cytotoxicity. , 1985, Annual review of immunology.

[3] R. Aebersold,et al. A natural killer cell granule protein that induces DNA fragmentation and apoptosis , 1992, The Journal of experimental medicine.

[4] M. Raff,et al. Programmed cell death and Bcl‐2 protection in the absence of a nucleus. , 1994, The EMBO journal.

[5] J. Cohen,et al. Differences in target cell DNA fragmentation induced by mouse cytotoxic T lymphocytes and natural killer cells. , 1986, Journal of immunology.

[6] E. Podack,et al. A central role of perforin in cytolysis? , 1991, Annual review of immunology.

[7] J. Cohen,et al. Endogenous endonuclease-induced DNA fragmentation: an early event in cell-mediated cytolysis. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[8] J. Russell,et al. Mechanisms of immune lysis. I. Physiological distinction between target cell death mediated by cytotoxic T lymphocytes and antibody plus complement. , 1980, Journal of immunology.

[9] J. Tite,et al. DNA fragmentation: manifestation of target cell destruction mediated by cytotoxic T-cell lines, lymphotoxin-secreting helper T-cell clones, and cell-free lymphotoxin-containing supernatant. , 1986, Proceedings of the National Academy of Sciences of the United States of America.