First report of viral infections that affect argentine honeybees.

Honey is one of the most important agricultural products for export in Argentina. In fact, more than 3.5 million beehives and 50 000 beekeepers are related with this production, mainly located in Buenos Aires province. Honeybee mortality is a serious problem that beekeepers in Argentina have had to face during the last 3 years. It is known that the consequence of the complex interactions between environmental and beekeeping parameters added to the effect of different disease agents such as viruses, bacteria, fungi and parasitic mites may result in a sudden collapse of the colony. In addition, multiple viral infections are frequently detected concomitantly in bee colonies. We describe here the preliminary results of a survey of three honeybee-pathogenic viruses, acute bee paralysis viruses (ABPV), chronic bee paralysis viruses (CBPV) and Sacbrood viruses (SBV) detected during a screening of 61 apiaries located in the main honey producer province using a RT-PCR assay. This is the first molecular report of the presence of these viruses in Argentine apiaries.

[1]  M. Higes,et al.  South American native bumblebees (Hymenoptera: Apidae) infected by Nosema ceranae (Microsporidia), an emerging pathogen of honeybees (Apis mellifera). , 2009, Environmental microbiology reports.

[2]  P. García-Palencia,et al.  Honeybee colony collapse due to Nosema ceranae in professional apiaries. , 2009, Environmental microbiology reports.

[3]  N. Sardella,et al.  First detection of Varroa destructor resistance to coumaphos in Argentina , 2009, Experimental and Applied Acarology.

[4]  J. Pettis,et al.  Virus infections in Brazilian honey bees. , 2008, Journal of invertebrate pathology.

[5]  D. Grasso,et al.  Evidence for plasmid-mediated tetracycline resistance in Paenibacillus larvae, the causal agent of American Foulbrood (AFB) disease in honeybees. , 2007, Veterinary microbiology.

[6]  P. Zunino,et al.  Honeybee viruses in Uruguay. , 2006, Journal of invertebrate pathology.

[7]  P. Zunino,et al.  Detection of chronic bee paralysis virus and acute bee paralysis virus in Uruguayan honeybees. , 2005, Journal of invertebrate pathology.

[8]  J. Ellis,et al.  The worldwide health status of honey bees , 2005 .

[9]  M. Bergoin,et al.  Prevalence and Seasonal Variations of Six Bee Viruses in Apis mellifera L. and Varroa destructor Mite Populations in France , 2004, Applied and Environmental Microbiology.

[10]  Yan Zhao,et al.  Multiple virus infections in the honey bee and genome divergence of honey bee viruses. , 2004, Journal of invertebrate pathology.

[11]  S. Davison,et al.  Detection of Acute Bee Paralysis Virus and Black Queen Cell Virus from Honeybees by Reverse Transcriptase PCR , 2001, Applied and Environmental Microbiology.

[12]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .