Survey of environmental biocontamination on board the International Space Station.

The International Space Station (ISS) is an orbital living and working environment extending from the original Zarya control module built in 1998. The expected life span of the completed station is around 10 years and during this period it will be constantly manned. It is inevitable that the ISS will also be home to an unknown number of microorganisms. This survey reports on microbiological contamination in potable water, air, and on surfaces inside the ISS. The viable counts in potable water did not exceed 1.0 x 10(2) CFU/ml. Sphingomonas sp. and Methylobacterium sp. were identified as the dominant genera. Molecular analysis demonstrated the presence of nucleic acids belonging to various pathogens, but no viable pathogens were recovered. More than 500 samples were collected at different locations over a period of 6 years to characterize air and surface contamination in the ISS. Concentrations of airborne bacteria and fungi were lower than 7.1 x 10(2) and 4.4 x 10(1) CFU/m3, respectively. Staphylococcus sp. was by far the most dominant airborne bacterial genus, whereas Aspergillus sp. and Penicillium sp. dominated the fungal population. The bacterial concentrations in surface samples fluctuated from 2.5 x 10(1) to 4.3 x 10(4) CFU/100 cm2. Staphylococcus sp. dominated in all of these samples. The number of fungi varied between 2.5 x 10(1) and 3.0 x 10(5) CFU/100 cm2, with Aspergillus sp. and Cladosporium sp. as the most dominant genera. Furthermore, the investigations identified the presence of several (opportunistic) pathogens and strains involved in the biodegradation of structural materials.

[1]  S. Fridkin,et al.  Epidemiology of nosocomial fungal infections , 1996, Clinical microbiology reviews.

[2]  A N Viktorov,et al.  Biological induced corrosion of materials II: new test methods and experiences from MIR station. , 1999, Acta astronautica.

[3]  K. Venkateswaran,et al.  Microbial Monitoring of Spacecraft and Associated Environments , 2004, Microbial Ecology.

[4]  John B. Shoven,et al.  I , Edinburgh Medical and Surgical Journal.

[5]  A N Viktorov,et al.  [Results of integrated tests of the facility to disinfect gaseous environment of orbital stations]. , 1996, Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine.

[6]  E. Boa,et al.  Ainsworth and Bisby's Dictionary of the Fungi , 1998 .

[7]  S S Yang,et al.  Microbiologically induced corrosion of aluminum alloys in fuel-oil/aqueous system. , 1998, Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi.

[8]  M. Roman,et al.  The role of microbial biofilms in deterioration of space station candidate materials. , 1998, International biodeterioration & biodegradation.

[9]  Kasthuri Venkateswaran,et al.  Evidence of pathogenic microbes in the International Space Station drinking water: reason for concern? , 2004, Habitation.

[10]  Ji-Dong Gu,et al.  Microbiological deterioration and degradation of synthetic polymeric materials: recent research advances , 2003 .

[11]  D. Pierson,et al.  Environmental mycology and its importance to public health. , 1992, Journal of medical and veterinary mycology : bi-monthly publication of the International Society for Human and Animal Mycology.

[12]  S. Makino,et al.  Application of the real-time PCR for the detection of airborne microbial pathogens in reference to the anthrax spores. , 2003, Journal of microbiological methods.

[13]  C Pasquarella,et al.  New methods for microbial contamination monitoring: an experiment on board the MIR orbital station. , 1997, Acta astronautica.

[14]  Kasthuri Venkateswaran,et al.  Characterization and monitoring of microbes in the international space station drinking water , 2003 .

[15]  D. Pierson,et al.  Microbial contamination of spacecraft. , 2001, Gravitational and space biology bulletin : publication of the American Society for Gravitational and Space Biology.

[16]  M. Ghannoum,et al.  Indoor Mold, Toxigenic Fungi, and Stachybotrys chartarum: Infectious Disease Perspective , 2003, Clinical Microbiology Reviews.

[17]  Viktorov An,et al.  Results of integrated tests of the facility to disinfect gaseous environment of orbital stations , 1996 .

[18]  Elena Deshevaya,et al.  The Results of Microbiological Research of Environmental Microflora of Orbital Station Mir , 2001 .

[19]  V Borisov,et al.  The "SCORPION" experiment onboard the International Space Station. Preliminary results. , 2003, Advances in space research : the official journal of the Committee on Space Research.

[20]  S. Parks,et al.  An assessment of the Sartorius MD8 microbiological air sampler. , 1996, The Journal of applied bacteriology.

[21]  G. Perrière,et al.  BIBI, a Bioinformatics Bacterial Identification Tool , 2003, Journal of Clinical Microbiology.

[22]  I. Dahllöf,et al.  Molecular community analysis of microbial diversity. , 2002, Current opinion in biotechnology.

[23]  D. Pierson,et al.  Microbial Characterization during the Early Habitation of the International Space Station , 2004, Microbial Ecology.

[24]  Jizhong Zhou.  Microarrays for bacterial detection and microbial community analysis. , 2003, Current opinion in microbiology.

[25]  N. D. Novikova,et al.  Review of the Knowledge of Microbial Contamination of the Russian Manned Spacecraft , 2004, Microbial Ecology.

[26]  L Birch,et al.  Molecular methods for the assessment of bacterial viability. , 2003, Journal of microbiological methods.

[27]  David E. Williams,et al.  International Space Station Environmental Control And Life Support System Status: 2001-2002 , 2002 .

[28]  James L. Baker,et al.  Ochratoxin Production by the Aspergillus ochraceus Group and Aspergillus alliaceus , 2002, Applied and Environmental Microbiology.