Physiopathological roles of spontaneously released outer membrane vesicles of Bordetella pertussis.

AIM Bordetella pertussis has been shown to release outer membrane vesicles (OMV) both in vitro and in vivo but little is known about their biological role during the initial phases of B. pertussis infection of the airways. RESULTS We have demonstrated that OMV are released by B. pertussis in a human ciliated-airway cell model and purified vesicles can interact with host cells. Binding and uptake are strictly Bvg-regulated and OMV-associated pertussis toxin contributes to host-cell intoxication. Furthermore, we have shown that OMV act as iron-delivery systems complementing the B. pertussis growth defect in iron-limiting conditions. CONCLUSION We have proved that OMV play different roles in B. pertussis physiopathology and we opened new perspectives to be further investigated.

[1]  Yao Wang,et al.  A Pseudomonas T6SS effector recruits PQS-containing outer membrane vesicles for iron acquisition , 2017, Nature Communications.

[2]  M. Pizza,et al.  A novel high-throughput assay to quantify the vaccine-induced inhibition of Bordetella pertussis adhesion to airway epithelia , 2016, BMC Microbiology.

[3]  M. Rumbo,et al.  Characterization of the immune response induced by pertussis OMVs-based vaccine. , 2016, Vaccine.

[4]  F. Mooi,et al.  Highly differentiated human airway epithelial cells: a model to study host cell–parasite interactions in pertussis , 2016, Infectious diseases.

[5]  M. Kuehn,et al.  Outer-membrane vesicles from Gram-negative bacteria: biogenesis and functions , 2015, Nature Reviews Microbiology.

[6]  C. Baldari,et al.  Neisseria meningitidis subverts the polarized organization and intracellular trafficking of host cells to cross the epithelial barrier , 2015, Cellular microbiology.

[7]  J. Pennings,et al.  Immunoproteomic Profiling of Bordetella pertussis Outer Membrane Vesicle Vaccine Reveals Broad and Balanced Humoral Immunogenicity. , 2015, Journal of proteome research.

[8]  M. Feldman,et al.  Prokaryotic membrane vesicles: new insights on biogenesis and biological roles , 2015, Biological chemistry.

[9]  M. Rumbo,et al.  Characterization of the key antigenic components of pertussis vaccine based on outer membrane vesicles. , 2014, Vaccine.

[10]  A. Casadevall,et al.  Characterization of protective extracellular membrane-derived vesicles produced by Streptococcus pneumoniae. , 2014, Journal of proteomics.

[11]  T. J. Brickman,et al.  Bordetella pertussis FbpA Binds Both Unchelated Iron and Iron Siderophore Complexes , 2014, Biochemistry.

[12]  S. Chisholm,et al.  Bacterial Vesicles in Marine Ecosystems , 2014, Science.

[13]  Daniel G. Piqué,et al.  Role for Mycobacterium tuberculosis Membrane Vesicles in Iron Acquisition , 2014, Journal of bacteriology.

[14]  M. Rumbo,et al.  Outer membrane vesicles derived from Bordetella parapertussis as an acellular vaccine against Bordetella parapertussis and Bordetella pertussis infection. , 2013, Vaccine.

[15]  M. E. Rodriguez,et al.  Bordetella pertussis iron regulated proteins as potential vaccine components. , 2013, Vaccine.

[16]  E. Chiappini,et al.  Pertussis re-emergence in the post-vaccination era , 2013, BMC Infectious Diseases.

[17]  D. Whitworth,et al.  Predatory activity of Myxococcus xanthus outer-membrane vesicles and properties of their hydrolase cargo. , 2012, Microbiology.

[18]  D. Hinton,et al.  The Bordetella pertussis model of exquisite gene control by the global transcription factor BvgA. , 2012, Microbiology.

[19]  E. Hewlett,et al.  Delivery of Bordetella pertussis adenylate cyclase toxin to target cells via outer membrane vesicles , 2012, FEBS letters.

[20]  J. Benach,et al.  The Enolase of Borrelia burgdorferi Is a Plasminogen Receptor Released in Outer Membrane Vesicles , 2011, Infection and Immunity.

[21]  D. Relman,et al.  Transcriptional Profiling of the Iron Starvation Response in Bordetella pertussis Provides New Insights into Siderophore Utilization and Virulence Gene Expression , 2011, Journal of Bacteriology.

[22]  K. Chaudhuri,et al.  Association of cholera toxin with Vibrio cholerae outer membrane vesicles which are internalized by human intestinal epithelial cells , 2011, FEBS letters.

[23]  D. Byrne,et al.  HmuY Haemophore and Gingipain Proteases Constitute a Unique Syntrophic System of Haem Acquisition by Porphyromonas gingivalis , 2011, PloS one.

[24]  P. A. van der Ley,et al.  Outer membrane vesicles obtained from Bordetella pertussis Tohama expressing the lipid A deacylase PagL as a novel acellular vaccine candidate. , 2011, Vaccine.

[25]  H. Takeuchi,et al.  Outer membrane vesicles function as offensive weapons in host-parasite interactions. , 2010, Microbes and infection.

[26]  M. Kuehn,et al.  Virulence and Immunomodulatory Roles of Bacterial Outer Membrane Vesicles , 2010, Microbiology and Molecular Biology Reviews.

[27]  M. Rumbo,et al.  Outer membrane vesicles as acellular vaccine against pertussis. , 2008, Vaccine.

[28]  M. Reedy,et al.  Enterotoxigenic Escherichia coli vesicles target toxin delivery into mammalian cells , 2004, The EMBO journal.

[29]  I. Barr,et al.  Characterization of a Novel Outer Membrane Hemin-Binding Protein of Porphyromonas gingivalis , 2000, Journal of bacteriology.

[30]  T. Cover,et al.  Release of Helicobacter pylori vacuolating cytotoxin by both a specific secretion pathway and budding of outer membrane vesicles. Uptake of released toxin and vesicles by gastric epithelium , 1999, The Journal of pathology.

[31]  A. Lagares,et al.  Release of Outer Membrane Vesicles from Bordetella pertussis , 1999, Current Microbiology.

[32]  R. Rappuoli,et al.  Adhesion of Bordetella pertussis to eukaryotic cells requires a time-dependent export and maturation of filamentous hemagglutinin. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[33]  L. Nencioni,et al.  Mutants of pertussis toxin suitable for vaccine development. , 1989, Science.

[34]  R. Rappuoli,et al.  Filamentous hemagglutinin of Bordetella pertussis: nucleotide sequence and crucial role in adherence. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[35]  J. Neilands,et al.  Universal chemical assay for the detection and determination of siderophores. , 1987, Analytical biochemistry.

[36]  E. Hewlett,et al.  Induction of a novel morphological response in Chinese hamster ovary cells by pertussis toxin , 1983, Infection and immunity.

[37]  S. Falkow,et al.  Transposon insertion and subsequent donor formation promoted by Tn501 in Bordetella pertussis , 1983, Journal of bacteriology.

[38]  D W Stainer,et al.  A simple chemically defined medium for the production of phase I Bordetella pertussis. , 1970, Journal of general microbiology.