Phospholipase C from Bacillus cereus and its use in studies of tissue thromboplastin.

A simplified method for the purification of phospholipase C from Bacillus cereus is described. The enzyme is homogeneous in disc electrophoresis and in dodecylsulphate-polyacrylamide gel electrophoresis as well as in the analytical ultracentrifuge. The enzyme had a molecular weight of 23000, pI= 6.5 and consisted of one subunit. A divalent metal ion was necessary, Zn2+ was the most active. The activation of factor VII by tissue thromboplastin was reversible when tissue thromboplastin was destroyed by phospholipase C.

[1]  B. Roelofsen,et al.  Complete purification and some properties of phospholipase C from Bacillus cereus. , 1971, Biochimica et biophysica acta.

[2]  S. Kinsky,et al.  Fate of phospholipids in liposomal model membranes damaged by antibody and complement. , 1970, Biochemistry.

[3]  H. Prydz,et al.  Purification of the coagulation factors VII and X from human serum. Some properties of factor VII. , 1970, Biochimica et biophysica acta.

[4]  M. Sheetz,et al.  On the interactions of lipids and proteins in the red blood cell membrane. , 1970, Proceedings of the National Academy of Sciences of the United States of America.

[5]  W. Lands,et al.  Purification of a phospholipase C from Bacillus cereus. , 1969, Biochimica et biophysica acta.

[6]  K. Weber,et al.  The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. , 1969, The Journal of biological chemistry.

[7]  H. Prydz,et al.  Studies on Tissue Thromboplastin - Its Splitting into Two Separable Parts , 1969, Thrombosis and Haemostasis.

[8]  H. Prydz,et al.  Studies on Tissue Thromboplastin - Electron Micrography , 1969, Thrombosis and Haemostasis.

[9]  N. Catsimpoolas.  Micro isoelectric focusing in polyacrylamide gel columns. , 1968, Analytical biochemistry.

[10]  P. Bonventre,et al.  Lethal Toxin of Bacillus cereus I. Relationships and Nature of Toxin, Hemolysin, and Phospholipase , 1967, Journal of bacteriology.

[11]  W. Wober,et al.  Trennung phosphorhaltiger Abbauprodukte von Phosphatiden auf Celluloseplatten , 1966 .

[12]  A. Ottolenghi.  Phospholipase C from Bacillus cereus, a zinc-requiring metalloenzyme. , 1965, Biochimica et biophysica acta.

[13]  M. Patterson,et al.  MEASUREMENT OF LOW ENERGY BETA-EMITTERS IN AQUEOUS SOLUTION BY LIQUID SCINTILLATION COUNTING OF EMULSIONS. , 1965, Analytical chemistry.

[14]  B. Davis.  DISC ELECTROPHORESIS – II METHOD AND APPLICATION TO HUMAN SERUM PROTEINS * , 1964, Annals of the New York Academy of Sciences.

[15]  D. A. Yphantis.  EQUILIBRIUM ULTRACENTRIFUGATION OF DILUTE SOLUTIONS. , 1964, Biochemistry.

[16]  P. S. Chen,et al.  Microdetermination of Phosphorus , 1956 .

[17]  D. Meranze,et al.  Thromboplastinase, a New Enzyme Which Destroys Thromboplastin , 1953, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.