Does intranasal application of zinc sulfate produce anosmia in the mouse? An olfactometric and anatomical study.

Mice pre-trained in an olfactometer were tested daily on odor detection and discrimination tasks after irrigation of their olfactory epithelium in each naris with 50 microl of 5% zinc sulfate or saline. Anterograde transport of a wheatgerm agglutinin-horseradish peroxidase (WGA-HRP) conjugate from the epithelium to the olfactory bulb was used to assess anatomical connectivity in these and in mice that were used only for histological analyses. One day after treatment, saline controls performed at high levels of accuracy in detecting vapor from solutions of 5-0.01% ethyl acetate and in an odor discrimination task but most ZnSO4-treated mice performed at chance for 5-30 days before showing recovery. Although dense WGA-HRP reaction product was found in the accessory olfactory bulb, there was little or no evidence for axonal transport to glomeruli of the main olfactory bulb in the first 4-8 days after treatment. These results demonstrate that intranasal application of ZnSO4 to mice produces a brief but essentially total disruption of functional connections from the olfactory epithelium to the main olfactory bulb and a corresponding transient anosmia.

[1]  D. H. Matulionis.  Light and electron microscopic study of the effects of ZnSO4 on mouse nasal respiratory epithelium and subsequent responses , 1975, The Anatomical record.

[2]  J. Alberts.  Producing and interpreting experimental olfactory deficits. , 1974, Physiology & behavior.

[3]  G. Nielsen,et al.  Effects of intranasal ZnSO4 irrigation on olfactory and trigeminal cues , 1994, Physiology & Behavior.

[4]  Wim E Crusio,et al.  Zinc-induced peripheral anosmia and behavioral responses to novelty in mice: a quantitative-genetic analysis. , 1987, Behavioral and neural biology.

[5]  P. Andiné,et al.  The effect of anosmia on MK-801-induced behaviour in mice , 1995, Neuroscience Letters.

[6]  D. A. Edwards,et al.  Olfactory bulb removal vs peripherally induced anosmia: differential effects on the aggressive behavior of male mice. , 1972, Behavioral biology.

[7]  Carrie L Iwema,et al.  Reinnervation of the rat olfactory bulb after methyl bromide‐induced lesion: Timing and extent of reinnervation , 1999, The Journal of comparative neurology.

[8]  G. Archunan,et al.  Stud Male Protection of Implantation in Food-deprived Mice: Evaluation of the Involvement of Olfactory-vomeronasal Systems , 1990, Experimental and clinical endocrinology.

[9]  G. Burd,et al.  Morphological study of the effects of intranasal zinc sulfate irrigation on the mouse olfactory epithelium and olfactory bulb , 1993, Microscopy research and technique.

[10]  N. Bean.  Modulation of agonistic behavior by the dual olfactory system in male mice , 1982, Physiology & Behavior.

[11]  W. E. Smith,et al.  Simultaneous and successive olfactory bulb removal: influences on the mating behavior of male mice. , 1973, Physiology & behavior.

[12]  Bruce F. Field,et al.  A multi-purpose photocell and touch circuit amplifier , 1987, Physiology & Behavior.

[13]  F. Margolis,et al.  Denervation in the primary olfactory pathway of mice. III. Effect on enzymes of carnosine metabolism , 1976, Brain Research.

[14]  J. Powers,et al.  Olfactory and vomeronasal deafferentation of male hamsters: Histological and behavioral analyses , 1977, Brain Research.

[15]  D. Ferriero,et al.  Denervation in the primary olfactory pathway of mice: biochemical and morphological effects. , 1974, Brain research.

[16]  Wim E Crusio,et al.  Zinc-induced peripheral anosmia and exploratory behavior in two inbred mouse strains , 1978, Physiology & Behavior.

[17]  D. A. Edwards,et al.  Olfactory control of the sexual behavior of male and female mice. , 1973, Physiology & behavior.

[18]  T. Getchell,et al.  Denervation of the primary olfactory pathway in mice. V. Long-term effect of intranasal ZnSO4 irrigation on behavior, biochemistry and morphology , 1978, Brain Research.

[19]  J. Vandenbergh.  Effects of central and peripheral anosmia on reproduction of female mice. , 1973, Physiology & behavior.

[20]  M. Tanida,et al.  Effects of olfactory stimulation on the sleep time induced by pentobarbital administration in mice , 1991, Brain Research Bulletin.

[21]  C. Wysocki,et al.  An ephemeral pheromone of female house mice: Perception via the main and accessory olfactory systems , 1995, Physiology & Behavior.

[22]  M. Grillo,et al.  Axoplasmic transport of carnosine (β-alanyl-L-histidine) in the mouse olfactory pathway , 1977, Neurochemical Research.

[23]  M. Mesulam,et al.  Tetramethyl benzidine for horseradish peroxidase neurohistochemistry: a non-carcinogenic blue reaction product with superior sensitivity for visualizing neural afferents and efferents. , 1978, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[24]  B. Gangrade,et al.  Effect of zinc sulphate-induced anosmia on estrous cycle of the laboratory mice. , 1983, Indian journal of experimental biology.

[25]  N. Bean.  Olfactory and vomeronasal mediation of ultrasonic vocalizations in male mice , 1982, Physiology & Behavior.

[26]  L. C. Drickamer.  Peripheral anosmia affects puberty-influencing chemosignals in mice: Donors and recipients , 1986, Physiology & Behavior.

[27]  R. Porter,et al.  Effects of intranasal ZnSO4 irrigation are mitigated by the presence of untreated littermates , 1987, Physiology & Behavior.

[28]  M. Furukawa,et al.  Effects of topical application of steroids on olfactory disturbance in mice , 1991 .

[29]  B. Slotnick,et al.  Does intranasal application of zinc sulfate produce anosmia in the rat? , 2000, Behavioral neuroscience.

[30]  R. Johnston.  Vomeronasal and/or olfactory mediation of ultrasonic calling and scent marking by female golden hamsters , 1992, Physiology & Behavior.

[31]  D. H. Matulionis.  Ultrastructural study of mouse olfactory epithelium following destruction by ZnSO4 and its subsequent regeneration. , 1975, The American journal of anatomy.

[32]  B. Slotnick,et al.  Performance of mice in an automated olfactometer: odor detection, discrimination and odor memory. , 1999, Chemical senses.

[33]  B. Galef,et al.  Acute anosmia in the rat: a behavioral test of a peripherally-induced olfactory deficit. , 1971, Physiology & behavior.

[34]  F. Bronson,et al.  Olfactory perception of the nonsocial environment by male house mice. , 1975, Behavioral biology.

[35]  M. Chuah,et al.  Response of olfactory Schwann cells to intranasal zinc sulfate irrigation , 1995, Journal of neuroscience research.

[36]  J. Matochik.  Role of the main olfactory system in recognition between individual spiny mice , 1988, Physiology & Behavior.

[37]  V. Denenberg,et al.  Maternal experience prevents pup-killing in mice induced by peripheral anosmia. , 1974, Physiology & behavior.

[38]  B. Galef,et al.  Olfactory cues and movement: stimuli mediating intraspecific aggression in the wild Norway rat. , 1973, Journal of comparative and physiological psychology.

[39]  B. Slotnick,et al.  Evaluation of intranasal zinc sulfate treatment on olfactory discrimination in rats. , 1977, Journal of comparative and physiological psychology.