Direct Determinationof TherapeuticConcentrationsof Lithiumin Serum by Flow-InjectionAnalysiswith AtomicAbsorptionSpectroscopicDetection

In this flow-Injection system for direct determination of lithium in serum by atomic absorption spectroscopy, the 10-rL sample is manually injected into a continuously flowing non-segmented stream of de-ionized water, which is pumped, via a dispersion tube, to the spectrometer’s nebulizer. Controlled dispersion of the sample zone, before It is introduced into the nebulizer, produces the required sample dilution. Effects of varying the length of the dispersion tube, the flow rate, and the sample size were studied. Analytical readout is obtained, in the form of transient peaks, 5 s after sample injection. ft is necessary to include physiological concentrations of sodium and potassium in the standards because each of these cations enhances the lithium absorbance signal. Analytical recovery (98.5 to 101 %) andCV (about 2%) are good, and resufts compare well with those obtained by aspiration of prediluted samples (n = 121, r = 0.99).

[1]  J. F. Staden,et al.  Simultaneous determination of sodium, potassium, magnesium and calcium in surface, ground and domestic water by flow-injection analysis , 1980 .

[2]  K. Ohls,et al.  Ergebnisse zur Zerstäubung dosierter Lösungsvolumina bei der AAS , 2015 .

[3]  B. F. Rocks,et al.  Flow-injection analysis , 1982 .

[4]  W. J. Price.  Spectrochemical Analysis by Atomic Absorption , 1979 .

[5]  J. Batsakis,et al.  Serum lithium. A CAP survey perspective. , 1977, American journal of clinical pathology.

[6]  A. Amdisen.  Serum Concentration and Clinical Supervision in Monitoring of Lithium Treatment , 1980, Therapeutic drug monitoring.

[7]  H. Coombs.  The Estimation of Lithium in Serum , 1971, British Journal of Psychiatry.

[8]  E. M. Katz,et al.  Comparison of serum lithium determinations by flame photometry and atomic absorption spectrophotomerry. , 1970, Clinical chemistry.

[9]  M. B. Denton,et al.  Microsampling Nebulizer Technique for Premixed Flame Atomic Spectrometry , 1978 .

[10]  J. Pybus,et al.  Measurement of serum lithium by atomic absorption spectroscopy. , 1970, Clinical chemistry.

[11]  H. J. v d. HELM,et al.  The determination of lithium in serum during therapy with lithium salts. , 1961, Clinica chimica acta; international journal of clinical chemistry.

[12]  P. E. Hare,et al.  Rapid analysis of discrete samples: the use of nonsegmented, continuous flow. , 1976, Analytical biochemistry.

[13]  H. S. Wright.  PRIMER OF LITHIUM THERAPY , 1979 .

[14]  H. Berndt,et al.  Atomabsorptions-spektrometrische bestimmung kleiner substanzmengen und analyse von Spurenkonzentrat-mit der “Injektionsmethode” , 1975 .

[15]  F. Krug,et al.  Merging zones in flow injection analysis: Part 2. Determination of Calcium, Magnesium and Potassium in Plant Material by Continuous Flow Injection Atomic Absorption and Flame Emission Spectrometry , 1979 .

[16]  K. Stewart,et al.  Automated multiple flow injection analysis for flame atomic absorption spectrometry , 1979 .

[17]  V. Lehmann.  Direct determination of lithium in serum by atomic absorption spectroscopy. , 1968, Clinica chimica acta; international journal of clinical chemistry.

[18]  R. Fieve,et al.  The measurement of lithium in biologic samples by atomic absorption spectrophotometry. , 1968, Clinical chemistry.