Effects of waterborne nano-iron on medaka (Oryzias latipes): antioxidant enzymatic activity, lipid peroxidation and histopathology.

Toxicity tests were performed to investigate possible harmful effects on medaka exposed to nano-iron. Dose-dependent decreases of superoxide dismutase (SOD) and increases of malondialdehyde (MDA) were induced in the medaka embryo, suggesting that oxidative damage was induced by nano-iron. For adult medaka, the disturbance of antioxidative balance was observed during the early exposure period based on the monitoring of the hepatic and cerebral SOD and reduced glutathione (GSH). No terminal oxidative damage occurred during the whole exposure period, probably due to the high self-recovering capability of the adult fish. Some histopathological and morphological alterations (cell swelling, hyperplasia, and granulomas, etc.) were observed in gill and intestine tissues, which confirmed that deleterious effects occurred as a result of direct contact with nano-iron. It is suggested that further evaluation should be made concerning the risk assessment of waterborne nano-iron on aquatic life.

[1]  V. Colvin.  The potential environmental impact of engineered nanomaterials , 2003, Nature Biotechnology.

[2]  K. Pantopoulos,et al.  Iron metabolism and toxicity. , 2005, Toxicology and applied pharmacology.

[3]  S. Bhatia,et al.  Probing the Cytotoxicity Of Semiconductor Quantum Dots. , 2004, Nano letters.

[4]  Y. Sun,et al.  A simple method for clinical assay of superoxide dismutase. , 1988, Clinical chemistry.

[5]  J. West,et al.  Nano-C60 cytotoxicity is due to lipid peroxidation. , 2005, Biomaterials.

[6]  C. Liao,et al.  Zero-valent iron reduction of nitrate in the presence of ultraviolet light, organic matter and hydrogen peroxide. , 2003, Water Research.

[7]  G. Ellman,et al.  Tissue sulfhydryl groups. , 1959, Archives of biochemistry and biophysics.

[8]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[9]  T. Webb,et al.  Comparative pulmonary toxicity assessment of single-wall carbon nanotubes in rats. , 2003, Toxicological sciences : an official journal of the Society of Toxicology.

[10]  S. Fernaeus,et al.  Increased iron-induced oxidative stress and toxicity in scrapie-infected neuroblastoma cells , 2005, Neuroscience Letters.

[11]  R. Nemanich,et al.  Multi-walled carbon nanotube interactions with human epidermal keratinocytes. , 2005, Toxicology letters.

[12]  L. Chiang,et al.  Acute and Subacute Toxicity Study of Water-Soluble Polyalkylsulfonated C60 in Rats , 1998, Toxicologic pathology.

[13]  N. Tanaka,et al.  The photogenotoxicity of titanium dioxide particles. , 1997, Mutation research.

[14]  Jeremy N Skepper,et al.  Effect of ultrasmall superparamagnetic iron oxide nanoparticles (Ferumoxtran-10) on human monocyte-macrophages in vitro. , 2007, Biomaterials.

[15]  Huajian Gao,et al.  Effect of single wall carbon nanotubes on human HEK293 cells. , 2005, Toxicology letters.

[16]  Mark Green,et al.  Semiconductor quantum dots and free radical induced DNA nicking. , 2005, Chemical communications.

[17]  Wei-xian Zhang,et al.  Synthesizing Nanoscale Iron Particles for Rapid and Complete Dechlorination of TCE and PCBs , 1997 .

[18]  R. Wilkin,et al.  High-level Arsenite Removal from Groundwater by Zero-valent Iron , 2022 .

[19]  J. Khim,et al.  Rapid reductive destruction of hazardous organic compounds by nanoscale Fe0. , 2001, Chemosphere.

[20]  Qamar Rahman,et al.  Evidence that ultrafine titanium dioxide induces micronuclei and apoptosis in Syrian hamster embryo fibroblasts. , 2002, Environmental health perspectives.

[21]  Vincent M Rotello,et al.  Toxicity of gold nanoparticles functionalized with cationic and anionic side chains. , 2004, Bioconjugate chemistry.

[22]  P. Aisen,et al.  Chemistry and biology of eukaryotic iron metabolism. , 2001, The international journal of biochemistry & cell biology.

[23]  Feng Zhao,et al.  Acute toxicological effects of copper nanoparticles in vivo. , 2006, Toxicology letters.

[24]  J. James,et al.  Pulmonary toxicity of single-wall carbon nanotubes in mice 7 and 90 days after intratracheal instillation. , 2003, Toxicological sciences : an official journal of the Society of Toxicology.

[25]  Heechul Choi,et al.  Removal of arsenic(III) from groundwater by nanoscale zero-valent iron. , 2005, Environmental science & technology.

[26]  E. Oberdörster.  Manufactured Nanomaterials (Fullerenes, C60) Induce Oxidative Stress in the Brain of Juvenile Largemouth Bass , 2004, Environmental health perspectives.

[27]  大川 博,et al.  Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction , 1979 .

[28]  K. Pinkerton,et al.  Pulmonary responses of acute exposure to ultrafine iron particles in healthy adult rats , 2003, Environmental toxicology.

[29]  R. Meneghini,et al.  Iron is the intracellular metal involved in the production of DNA damage by oxygen radicals. , 1991, Mutation research.