The production of chronic renal disease in rats by a single intravenous injection of aminonucleoside of puromycin and the effect of low dosage continuous hydrocortisone.

Lendrum's acid picro-Mallory and the periodic acid methenamine silver method. Other tissues were stained by H. and E., V"an Gieson and PAS. For electron microscopy, kidney tissue was fixed in buffered osmium tetroxide and emnbedded in methacrylate or Araldite (CIBA). In one animal from each group blood pressure estimations were made at 48 weeks by direct cannulation of the femoral arteries under ether anaesthesia before being killed. All animals were fed standard diets and were weighed at regular intervals. The experiment was continued for 60 weeks.