Identification of Estrogen Receptor β2, A Functional Variant of Estrogen Receptor β Expressed in Normal Rat Tissues.

The effects of estrogen and estrogen agonists can be mediated by estrogen receptor α (ERα) and estrogen receptor β (ERβ). We now report the identification and initial characterization of several novel isoforms of rat ERβ messenger RNA (mRNA). The most abundant of these mRNA variants we have called ERβ2. ERβ2 had an in-frame insertion of 54 nucleotides that resulted in the predicted insertion of 18 amino acids within the ligand binding domain. We demonstrated by semiquantitative RT-PCR and RNase protection that ERβ2 mRNA was expressed at levels equal to those of the previously published ERβ (ERβ1) in ovary, prostate, pituitary, and muscle. In tissues of the nervous system, including frontal cortex, hippocampus, and hypothalamus, ERβ1 was present in a 2- to 6-fold greater abundance than ERβ2. We have also detected variants of both ERβ1 and ERβ2 mRNAs that contained deletions of 117 bp encompassing the region encoding the second zinc finger of the DNA binding domain. All four mRNA species were efficiently tr...

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