Expression and modulation of voltage-gated calcium channels after RNA injection in Xenopus oocytes.

Calcium ions flow into cells through several distinct classes of voltage-dependent calcium-selective channels. Such fluxes play important roles in electrical signaling at the cell membrane and in chemical signaling within cells. Further information about calcium channels was obtained by injecting RNA isolated from rat brain, heart and skeletal muscle into Xenopus oocytes. Macroscopic currents through voltage-operated calcium channels were resolved when the endogenous calcium-dependent chloride current was blocked by replacing external calcium with barium and chloride with methanesulfonate. The resulting barium current was insensitive to tetrodotoxin but was completely blocked by cadmium or cobalt. With both heart and brain RNA at least two distinct types of calcium ion conductance were found, distinguishable by their time course and inactivation properties. In oocytes injected with heart RNA, the slowly inactivating component was selectively blocked by the calcium-channel antagonist nifedipine. Barium ion currents induced by heart RNA were modulated by isoproterenol, cyclic adenosine monophosphate, and acetylcholine.

[1]  Y. Oron,et al.  Adenosine-induced K+ current in Xenopus oocyte and the role of adenosine 3',5'-monophosphate. , 1985, Molecular pharmacology.

[2]  R. Tsien,et al.  A novel type of cardiac calcium channel in ventricular cells , 1985, Nature.

[3]  R. Tsien,et al.  Three types of neuronal calcium channel with different calcium agonist sensitivity , 1985, Nature.

[4]  B. Bean.  Two kinds of calcium channels in canine atrial cells. Differences in kinetics, selectivity, and pharmacology , 1985, The Journal of general physiology.

[5]  P. Kostyuk,et al.  Two types of calcium channels in the somatic membrane of new‐born rat dorsal root ganglion neurones. , 1985, The Journal of physiology.

[6]  J. Stinnakre,et al.  β-Adrenergic induced K+ current in Xenopus oocytes: role of cAMP, inhibition by muscarinic agents , 1985, Proceedings of the Royal Society of London. Series B. Biological Sciences.

[7]  Y. Oron,et al.  Inositol 1,4,5-trisphosphate mimics muscarinic response in Xenopus oocytes , 1985, Nature.

[8]  A. Cm,et al.  Two distinct populations of calcium channels in a clonal line of pituitary cells. , 1985 .

[9]  A. Fox,et al.  Two calcium currents in Neanthes arenaceodentatus egg cell membranes. , 1984, The Journal of physiology.

[10]  M. Lazdunski,et al.  Purification of the dihydropyridine receptor of the voltage-dependent Ca2+ channel from skeletal muscle transverse tubules using (+) [3H]PN 200-110. , 1984, Biochemical and biophysical research communications.

[11]  N. Dascal,et al.  Xenopus oocyte resting potential, muscarinic responses and the role of calcium and guanosine 3',5'‐cyclic monophosphate. , 1984, The Journal of physiology.

[12]  D. A. Brown,et al.  Expression of functional GABA, glycine and glutamate receptors in Xenopus oocytes injected with rat brain mRNA , 1984, Nature.

[13]  W. Catterall,et al.  Purification of the calcium antagonist receptor of the voltage-sensitive calcium channel from skeletal muscle transverse tubules. , 1984, Biochemistry.

[14]  R. Miledi,et al.  Messenger RNA from human brain induces drug- and voltage-operated channels in Xenopus oocytes , 1984, Nature.

[15]  M. Lazdunski,et al.  Ontogenic appearance of Ca2+ channels characterized as binding sites for nitredipine during development of nervous, skeletal and cardiac muscle systems in the rat , 1983, FEBS letters.

[16]  R. Tsien.  Calcium channels in excitable cell membranes. , 1983, Annual review of physiology.

[17]  M E Barish,et al.  A transient calcium‐dependent chloride current in the immature Xenopus oocyte. , 1983, The Journal of physiology.

[18]  W. Catterall,et al.  Solubilization of the calcium antagonist receptor from rat brain. , 1983, The Journal of biological chemistry.

[19]  H. Reuter.  Calcium channel modulation by neurotransmitters, enzymes and drugs , 1983, Nature.

[20]  R. Miledi,et al.  A calcium-dependent transient outward current in Xenopus laevis oocytes , 1982, Proceedings of the Royal Society of London. Series B. Biological Sciences.

[21]  R. Miledi,et al.  Cholinergic and catecholaminergic receptors in the Xenopus oocyte membrane , 1982, The Journal of physiology.

[22]  N. Dascal,et al.  Types of muscarinic response in Xenopus oocytes. , 1980, Life sciences.

[23]  R. Miledi,et al.  Acetylcholine receptors in the oocyte membrane , 1977, Nature.

[24]  L. D. Smith,et al.  Inhibition of oocyte maturation by theophylline: possible mechanism of action. , 1976, Developmental biology.

[25]  J. Dumont.  Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals , 1972, Journal of morphology.

[26]  J. Gurdon,et al.  Use of Frog Eggs and Oocytes for the Study of Messenger RNA and its Translation in Living Cells , 1971, Nature.