Effect of sunflower oil Supplementation on in vitro Fermentation Patterns of Forage Based Diets for Ruminant

The aim of present study was to investigation on influence of supplemented sunflower oil (SFO) in vitro fermentation of forage based mixed ration. Alfalfa forage and barley grain (as concentrated feed) were mixed to obtaining total mixed rations with 60 to 40% and 50 to 50% respectively for forage and concentrated portions. The SFO in the levels of 0, 2.5 or 5% of ration was added to mixed rations. Three fistulaed native bulls fed experimental rations twice daily for 15 days, and ruminal fluid was collected. Gas production of in vitro fermentation was measured as the volume of gas in the calibrated syringes and was recorded before incubation 2, 4, 6, 8, 12, 24, 48, 72 and 96 hours after incubation. Total gas values were corrected for blank incubation which contained only rumen fluid. There was no any significant deference treatments (0, 2.5 or 5% SFO), whereas there was significant differences between two basal rations. The addition of vegetable oil to forage based ration didn’t affect gas production parameters. It was concluded that, supplementation of sunflower oil to foragebased rations didn’t affect gas production parameters, and in higher concentration may be inadvisable for ruminal fermentation.

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