In situ hybridization of PNA/DNA studied label-free by electrochemical impedance spectroscopy.

The in situ hybridization kinetics of label-free DNA on mixed monolayers of peptide nucleic acid (PNA) and 6-mercapto-1-hexanol (MCH) on Au electrodes was investigated by electrochemical impedance spectroscopy (EIS) and used to discriminate the fully complementary DNA from the single-base mismatched hybrids.

[1]  Wolfgang Knoll,et al.  Oligonucleotide hybridization observed by surface plasmon optical techniques , 1995 .

[2]  Itamar Willner,et al.  Enzyme-Linked Amplified Electrochemical Sensing of Oligonucleotide−DNA Interactions by Means of the Precipitation of an Insoluble Product and Using Impedance Spectroscopy , 1999 .

[3]  M. Egholm,et al.  Sequence-selective recognition of DNA by strand displacement with a thymine-substituted polyamide. , 1991, Science.

[4]  B. Nordén,et al.  Kinetics for hybridization of peptide nucleic acids (PNA) with DNA and RNA studied with the BIAcore technique. , 1997, Biochemistry.

[5]  I. Willner,et al.  Liposomes labeled with biotin and horseradish peroxidase: a probe for the enhanced amplification of antigen--antibody or oligonucleotide--DNA sensing processes by the precipitation of an insoluble product on electrodes. , 2001, Analytical chemistry.

[6]  Chad A. Mirkin,et al.  Programmed Materials Synthesis with DNA. , 1999, Chemical reviews.

[7]  G. Maracas,et al.  Impedance labelless detection-based polypyrrole DNA biosensor. , 2005, Frontiers in bioscience : a journal and virtual library.

[8]  I. Willner,et al.  Electronic transduction of DNA sensing processes on surfaces: amplification of DNA detection and analysis of single-base mismatches by tagged liposomes. , 2001, Journal of the American Chemical Society.

[9]  Yi-Tao Long,et al.  AC impedance spectroscopy of native DNA and M-DNA. , 2003, Biophysical journal.

[10]  E. Southern,et al.  Determining the influence of structure on hybridization using oligonucleotide arrays , 1999, Nature Biotechnology.

[11]  W. Knoll,et al.  Investigating the kinetics of DNA-DNA and PNA-DNA interactions using surface plasmon resonance-enhanced fluorescence spectroscopy. , 2001, Biosensors & bioelectronics.

[12]  W. Knoll,et al.  Complement hybridization from solution to surface-attached probe-oligonucleotides observed by surface-plasmon-field-enhanced fluorescence spectroscopy , 2000 .

[13]  Joseph Wang.  SURVEY AND SUMMARY From DNA biosensors to gene chips , 2000 .

[14]  R. Georgiadis,et al.  Quantitative measurements and modeling of kinetics in nucleic acid monolayer films using SPR spectroscopy , 2000 .

[15]  I. Willner,et al.  Probing Biomolecular Interactions at Conductive and Semiconductive Surfaces by Impedance Spectroscopy: Routes to Impedimetric Immunosensors, DNA‐Sensors, and Enzyme Biosensors , 2003 .

[16]  I. Willner,et al.  Highly sensitive amplified electronic detection of DNA by biocatalyzed precipitation of an insoluble product onto electrodes. , 2003, Chemistry.

[17]  W. Knoll,et al.  Surface-plasmon fluorescence spectroscopy , 2002 .

[18]  Ulrich J. Krull,et al.  Fiber optic biosensor for fluorimetric detection of DNA hybridization , 1994 .

[19]  I. Willner,et al.  Chronopotentiometry and Faradaic impedance spectroscopy as signal transduction methods for the biocatalytic precipitation of an insoluble product on electrode supports: routes for enzyme sensors, immunosensors and DNA sensors. , 2001, Biosensors & bioelectronics.

[20]  Chen-zhong Li,et al.  Protein-DNA interaction: impedance study of MutS binding to a DNA mismatch. , 2004, Chemical communications.