Modern Approaches to Differentiation of Live and Dead Bacteria Using Selective Amplification of Nucleic Acids

[1]  S. Fukuzawa,et al.  Selective Detection of DNA from Viable Mycobacterium tuberculosis Complex Strains Using the EMA-PCR Method. , 2019, Japanese journal of infectious diseases (Print).

[2]  C. Zi,et al.  An improved assay for rapid detection of viable Staphylococcus aureus cells by incorporating surfactant and PMA treatments in qPCR , 2018, BMC Microbiology.

[3]  Jing Chen,et al.  Development of propidium monoazide-recombinase polymerase amplification (PMA-RPA) assay for rapid detection of Streptococcus pyogenes and Streptococcus agalactiae. , 2018, Molecular and cellular probes.

[4]  T. Wiele,et al.  Comparison of conventional plating, PMA-qPCR, and flow cytometry for the determination of viable enterotoxigenic Escherichia coli along a gastrointestinal in vitro model , 2018, Applied Microbiology and Biotechnology.

[5]  Qiang Chen,et al.  Flow cytometry-based method facilitates optimization of PMA treatment condition for PMA-qPCR method. , 2018, Molecular and cellular probes.

[6]  D. Qu,et al.  Propidium monoazide real‐time loop‐mediated isothermal amplification for specific visualization of viable Salmonella in food , 2018, Letters in applied microbiology.

[7]  Jiaqi Wang,et al.  Quantitative PCR coupled with sodium dodecyl sulfate and propidium monoazide for detection of viable Staphylococcus aureus in milk. , 2018, Journal of dairy science.

[8]  Seunguk Lee,et al.  Evaluating the newly developed dye, DyeTox13 Green C-2 Azide, and comparing it with existing EMA and PMA for the differentiation of viable and nonviable bacteria. , 2018, Journal of microbiological methods.

[9]  Mickaël Boyer,et al.  Droplet digital PCR improves absolute quantification of viable lactic acid bacteria in faecal samples. , 2018, Journal of microbiological methods.

[10]  R. Niessner,et al.  Quantification of viable and non-viable Legionella spp. by heterogeneous asymmetric recombinase polymerase amplification (haRPA) on a flow-based chemiluminescence microarray. , 2018, Biosensors & bioelectronics.

[11]  Seunguk Lee,et al.  Molecular viability testing of viable but non‐culturable bacteria induced by antibiotic exposure , 2017, Microbial biotechnology.

[12]  Bernd Appel,et al.  Improved sample treatment protocol for accurate detection of live Salmonella spp. in food samples by viability PCR , 2017, PloS one.

[13]  N. Piqué,et al.  Viability qPCR, a new tool for Legionella risk management. , 2017, International journal of hygiene and environmental health.

[14]  Hengyi Xu,et al.  Rapid and simultaneous quantification of viable Escherichia coli O157:H7 and Salmonella spp. in milk through multiplex real-time PCR. , 2017, Journal of dairy science.

[15]  Ling Yang,et al.  The novel loop-mediated isothermal amplification based confirmation methodology on the bacteria in Viable but Non-Culturable (VBNC) state. , 2017, Microbial pathogenesis.

[16]  Wentao Xu,et al.  Ultrasensitive Detection of Viable Enterobacter sakazakii by a Continual Cascade Nanozyme Biosensor. , 2017, Analytical chemistry.

[17]  W. Khan,et al.  Comparison of EMA-, PMA- and DNase qPCR for the determination of microbial cell viability , 2017, Applied Microbiology and Biotechnology.

[18]  A. Nocker,et al.  The feasibility of improved live-dead distinction in qPCR-based microbial source tracking. , 2017, Journal of microbiological methods.

[19]  P. Huck,et al.  Application of long amplicon propidium monoazide-PCR to assess the effects of temperature and background microbiota on pathogens in river water. , 2017, Journal of water and health.

[20]  Yujun Jiang,et al.  Salmonella detection in powdered dairy products using a novel molecular tool. , 2017, Journal of dairy science.

[21]  Zhenwen Zhou,et al.  PMA-LAMP for rapid detection of Escherichia coli and shiga toxins from viable but non-culturable state. , 2017, Microbial pathogenesis.

[22]  J. Meschke,et al.  Molecular Viability Testing of UV-Inactivated Bacteria , 2017, Applied and Environmental Microbiology.

[23]  M. Fittipaldi,et al.  False-Positive Viability PCR Results: An Association with Microtubes , 2017, Current Microbiology.

[24]  S. C. Jung,et al.  Application of loop‐mediated isothermal amplification with propidium monoazide treatment to detect live Salmonella in chicken carcasses , 2017, Poultry science.

[25]  C. Willers,et al.  Efflux as a mechanism of antimicrobial drug resistance in clinical relevant microorganisms: the role of efflux inhibitors , 2017, Expert opinion on therapeutic targets.

[26]  C. Hoebe,et al.  Viability-PCR Shows That NAAT Detects a High Proportion of DNA from Non-Viable Chlamydia trachomatis , 2016, PloS one.

[27]  T. Soejima,et al.  Innovative Use of Palladium Compounds To Selectively Detect Live Enterobacteriaceae in Milk by PCR , 2016, Applied and Environmental Microbiology.

[28]  Jin-zhong Xiao,et al.  Innovative use of platinum compounds to selectively detect live microorganisms by polymerase chain reaction , 2016, Biotechnology and bioengineering.

[29]  Haiyan Li,et al.  Multiplex PMA-qPCR Assay with Internal Amplification Control for Simultaneous Detection of Viable Legionella pneumophila, Salmonella typhimurium, and Staphylococcus aureus in Environmental Waters. , 2015, Environmental science & technology.

[30]  Man-Ho Lee,et al.  Evaluation of propidium monoazide-quantitative PCR to detect viable Mycobacterium fortuitum after chlorine, ozone, and ultraviolet disinfection. , 2015, International journal of food microbiology.

[31]  R. Levin,et al.  Application of ethidium bromide monoazide for quantification of viable and dead cells of Salmonella enterica by real-time loop-mediated isothermal amplification. , 2015, Journal of microbiological methods.

[32]  Anna Allué-Guardia,et al.  Cell membrane integrity and distinguishing between metabolically active and inactive cells as a means of improving viability PCR. , 2015, Molecular and cellular probes.

[33]  Yulia V. Dubrovskaya,et al.  Utility of propidium monoazide viability assay as a biomarker for a tuberculosis disease. , 2015, Tuberculosis.

[34]  D. Pinto,et al.  Thirty years of viable but nonculturable state research: Unsolved molecular mechanisms , 2015, Critical reviews in microbiology.

[35]  C. Zotti,et al.  Overestimation of the Legionella spp. load in environmental samples by quantitative real-time PCR: pretreatment with propidium monoazide as a tool for the assessment of an association between Legionella concentration and sanitary risk. , 2014, Diagnostic microbiology and infectious disease.

[36]  J. Meschke,et al.  Dead or Alive: Molecular Assessment of Microbial Viability , 2014, Applied and Environmental Microbiology.

[37]  C. Gill,et al.  Use of sodium lauroyl sarcosinate (sarkosyl) in viable real-time PCR for enumeration of Escherichia coli. , 2014, Journal of microbiological methods.

[38]  Feng Xu,et al.  Rapid and accurate detection of viable Escherichia coli O157:H7 in milk using a combined IMS, sodium deoxycholate, PMA and real-time quantitative PCR process , 2014 .

[39]  T. Schwartz,et al.  DNase I and Proteinase K eliminate DNA from injured or dead bacteria but not from living bacteria in microbial reference systems and natural drinking water biofilms for subsequent molecular biology analyses. , 2013, Journal of microbiological methods.

[40]  Feng Xu,et al.  Magnetic nano-beads based separation combined with propidium monoazide treatment and multiplex PCR assay for simultaneous detection of viable Salmonella Typhimurium, Escherichia coli O157:H7 and Listeria monocytogenes in food products. , 2013, Food microbiology.

[41]  W. Hui,et al.  Molecular monitoring of Escherichia coli O157: H7 sterilization rate using qPCR and propidium monoazide treatment , 2013, Letters in applied microbiology.

[42]  R. Balasubramanian,et al.  Discrimination of viable from non-viable gram-negative bacterial pathogens in airborne particles using propidium monoazide-assisted qPCR. , 2013, The Science of the total environment.

[43]  B. Martín,et al.  Effect of Amplicon Length in Propidium Monoazide Quantitative PCR for the Enumeration of Viable Cells of Salmonella in Cooked Ham , 2013, Food Analytical Methods.

[44]  A. Nocker,et al.  Use of propidium monoazide and increased amplicon length reduce false-positive signals in quantitative PCR for bioburden analysis , 2013, Applied Microbiology and Biotechnology.

[45]  Mariana Fittipaldi,et al.  Progress in understanding preferential detection of live cells using viability dyes in combination with DNA amplification. , 2012, Journal of microbiological methods.

[46]  P. Huck,et al.  Long‐amplicon propidium monoazide‐PCR enumeration assay to detect viable Campylobacter and Salmonella , 2012, Journal of applied microbiology.

[47]  J. Yaguchi,et al.  Enumeration of viable Escherichia coli by real-time PCR with propidium monoazide. , 2012, Water Science and Technology.

[48]  T. Soejima,et al.  An advanced PCR method for the specific detection of viable total coliform bacteria in pasteurized milk , 2012, Applied Microbiology and Biotechnology.

[49]  Gérard Lina,et al.  Evaluation of propidium monoazide (PMA) treatment directly on membrane filter for the enumeration of viable but non cultivable Legionella by qPCR. , 2012, Journal of microbiological methods.

[50]  J. Chae,et al.  Selective detection of viable Helicobacter pylori using ethidium monoazide or propidium monoazide in combination with real‐time polymerase chain reaction , 2011, Microbiology and immunology.

[51]  S. Yoshida,et al.  Rapid detection of viable bacteria by nested polymerase chain reaction via long DNA amplification after ethidium monoazide treatment. , 2011, Analytical biochemistry.

[52]  S. Yoshida,et al.  Polymerase chain reaction amplification length-dependent ethidium monoazide suppression power for heat-killed cells of Enterobacteriaceae. , 2011, Analytical biochemistry.

[53]  H. Urrutia,et al.  Effect of PCR amplicon length on suppressing signals from membrane-compromised cells by propidium monoazide treatment. , 2011, Journal of microbiological methods.

[54]  W. Teughels,et al.  Live/dead real-time polymerase chain reaction to assess new therapies against dental plaque-related pathologies. , 2011, Molecular oral microbiology.

[55]  H. Davey.  Life, Death, and In-Between: Meanings and Methods in Microbiology , 2011, Applied and Environmental Microbiology.

[56]  Maria Befring Hovda,et al.  Propidium monoazide combined with real-time quantitative PCR underestimates heat-killed Listeria innocua. , 2011, Journal of microbiological methods.

[57]  A. Nocker,et al.  Quantification of viable Legionella pneumophila cells using propidium monoazide combined with quantitative PCR. , 2011, Journal of Microbiological Methods.

[58]  Fei Wang,et al.  Rapid Detection of Viable Salmonellae in Produce by Coupling Propidium Monoazide with Loop-Mediated Isothermal Amplification , 2011, Applied and Environmental Microbiology.

[59]  T. Soejima,et al.  New approach to use ethidium bromide monoazide as an analytical tool , 2010, Journal of applied microbiology.

[60]  J. Oliver,et al.  Recent findings on the viable but nonculturable state in pathogenic bacteria. , 2010, FEMS microbiology reviews.

[61]  Haruo Watanabe,et al.  Comparison of ethidium monoazide and propidium monoazide for the selective detection of viable Legionella cells. , 2010, Japanese journal of infectious diseases.

[62]  J. Meschke,et al.  Molecular Detection of Viable Bacterial Pathogens in Water by Ratiometric Pre-rRNA Analysis , 2009, Applied and Environmental Microbiology.

[63]  Lin Li,et al.  Specific Detection of Viable Salmonella Cells by an Ethidium Monoazide-Loop Mediated Isothermal Amplification (EMA-LAMP) Method , 2009 .

[64]  T. Bauer,et al.  Limiting false-positive polymerase chain reaction results: detection of DNA and mRNA to differentiate viable from dead bacteria. , 2009, Diagnostic microbiology and infectious disease.

[65]  D. Wahman,et al.  Monochloramine Disinfection Kinetics of Nitrosomonas europaea by Propidium Monoazide Quantitative PCR and Live/Dead BacLight Methods , 2009, Applied and Environmental Microbiology.

[66]  C. Dock,et al.  PCR-Based Method Using Propidium Monoazide To Distinguish Viable from Nonviable Bacillus subtilis Spores , 2009, Applied and Environmental Microbiology.

[67]  S. Wuertz,et al.  Discrimination of Viable and Dead Fecal Bacteroidales Bacteria by Quantitative PCR with Propidium Monoazide , 2009, Applied and Environmental Microbiology.

[68]  A. Nocker,et al.  Selective detection of live bacteria combining propidium monoazide sample treatment with microarray technology. , 2009, Journal of microbiological methods.

[69]  R. Witthuhn,et al.  Selective PCR detection of viable Enterobacter sakazakii cells utilizing propidium monoazide or ethidium bromide monoazide , 2008, Journal of applied microbiology.

[70]  S. Soda,et al.  Application of real-time polymerase chain reaction (PCR) coupled with ethidium monoazide treatment for selective quantification of viable bacteria in aquatic environment. , 2008, Water science and technology : a journal of the International Association on Water Pollution Research.

[71]  S. Yoshida,et al.  Method To Detect Only Live Bacteria during PCR Amplification , 2008, Journal of Clinical Microbiology.

[72]  B. Knapp,et al.  Removal of Free Extracellular DNA from Environmental Samples by Ethidium Monoazide and Propidium Monoazide , 2008, Applied and Environmental Microbiology.

[73]  S. Vesper,et al.  Quantifying fungal viability in air and water samples using quantitative PCR after treatment with propidium monoazide (PMA). , 2008, Journal of microbiological methods.

[74]  J. Pisz,et al.  Differentiation of genes extracted from non-viable versus viable micro-organisms in environmental samples using ethidium monoazide bromide. , 2007, Journal of microbiological methods.

[75]  F. Breidt,et al.  Enumeration of Viable Listeria monocytogenes Cells by Real-Time PCR with Propidium Monoazide and Ethidium Monoazide in the Presence of Dead Cells , 2007, Applied and Environmental Microbiology.

[76]  A. Nocker,et al.  Molecular monitoring of disinfection efficacy using propidium monoazide in combination with quantitative PCR. , 2007, Journal of microbiological methods.

[77]  A. Takade,et al.  Photoactivated Ethidium Monoazide Directly Cleaves Bacterial DNA and Is Applied to PCR for Discrimination of Live and Dead Bacteria , 2007, Microbiology and immunology.

[78]  F. Smulders,et al.  Insufficient differentiation of live and dead Campylobacter jejuni and Listeria monocytogenes cells by ethidium monoazide (EMA) compromises EMA/real-time PCR. , 2007, Research in microbiology.

[79]  Tsugunori Notomi,et al.  [Loop-mediated isothermal amplification]. , 2007, Nihon rinsho. Japanese journal of clinical medicine.

[80]  G. Skogan,et al.  Detection of Vibrio cholerae by Real-Time Nucleic Acid Sequence-Based Amplification , 2007, Applied and Environmental Microbiology.

[81]  Anne K Camper,et al.  Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cells. , 2006, Journal of microbiological methods.

[82]  M. Wagner,et al.  Possible Errors in the Interpretation of Ethidium Bromide and PicoGreen DNA Staining Results from Ethidium Monoazide-Treated DNA , 2006, Applied and Environmental Microbiology.

[83]  Anne K. Camper,et al.  Selective Removal of DNA from Dead Cells of Mixed Bacterial Communities by Use of Ethidium Monoazide , 2006, Applied and Environmental Microbiology.

[84]  Knut Rudi,et al.  Use of Ethidium Monoazide and PCR in Combination for Quantification of Viable and Dead Cells in Complex Samples , 2005, Applied and Environmental Microbiology.

[85]  Knut Rudi,et al.  Ethidium monoazide for DNA-based differentiation of viable and dead bacteria by 5'-nuclease PCR. , 2003, BioTechniques.

[86]  C. E. Dawson,et al.  A comparison of nucleic acid amplification techniques for the assessment of bacterial viability , 2001, Letters in applied microbiology.

[87]  C. Signoretto,et al.  mRNA Detection by Reverse Transcription-PCR for Monitoring Viability over Time in an Enterococcus faecalis Viable but Nonculturable Population Maintained in a Laboratory Microcosm , 2000, Applied and Environmental Microbiology.

[88]  J. Hays,et al.  An RNA transcription‐based amplification technique (NASBA) for the detection of viable Salmonella enterica , 2000, Letters in applied microbiology.

[89]  M. Perkins,et al.  Detection of Viable Mycobacterium tuberculosis by Reverse Transcriptase-Strand Displacement Amplification of mRNA. , 1999, Methods in molecular medicine.

[90]  Lee-Ann Jaykus,et al.  rRNA Stability in Heat-Killed and UV-Irradiated EnterotoxigenicStaphylococcus aureus and Escherichia coliO157:H7 , 1998, Applied and Environmental Microbiology.

[91]  Stephens,et al.  Assessment of bacterial viability status by flow cytometry and single cell sorting , 1998, Journal of applied microbiology.

[92]  B. Mackey,et al.  Detection of mRNA by Reverse Transcription-PCR as an Indicator of Viability in Escherichia coliCells , 1998, Applied and Environmental Microbiology.

[93]  D. Kell,et al.  Viability and activity in readily culturable bacteria: a review and discussion of the practical issues , 1998, Antonie van Leeuwenhoek.

[94]  V. Juneja,et al.  Sensitive detection of viable Listeria monocytogenes by reverse transcription-PCR , 1997, Applied and environmental microbiology.

[95]  G. Cangelosi,et al.  Detection of rifampin- and ciprofloxacin-resistant Mycobacterium tuberculosis by using species-specific assays for precursor rRNA , 1996, Antimicrobial agents and chemotherapy.

[96]  Daniel D. Jones,et al.  Detection of viableVibrio cholerae by reverse-transcriptase polymerase chain reaction (RT-PCR) , 1996, Molecular biotechnology.

[97]  B. P. Belotserkovskii,et al.  Polypropylene Tube Surfaces May Induce Denaturation and Multimerization of DNA , 1996, Science.

[98]  P. Klatser,et al.  Assessment of mycobacterial viability by RNA amplification , 1994, Antimicrobial Agents and Chemotherapy.

[99]  R M Atlas,et al.  Effect of amplicon size on PCR detection of bacteria exposed to chlorine. , 1993, PCR methods and applications.

[100]  H. Dockrell,et al.  Use of polymerase chain reaction to assess efficacy of leprosy chemotherapy , 1993, The Lancet.

[101]  A. Abe,et al.  A sensitive method for the detection of enterotoxigenic Escherichia coli by the polymerase chain reaction using multiple primer pairs. , 1992, Zentralblatt fur Bakteriologie : international journal of medical microbiology.

[102]  R M Atlas,et al.  Detection of viable Legionella pneumophila in water by polymerase chain reaction and gene probe methods , 1991, Applied and environmental microbiology.

[103]  T. Schwan,et al.  A specific and sensitive assay for the Lyme disease spirochete Borrelia burgdorferi using the polymerase chain reaction. , 1989, The Journal of infectious diseases.

[104]  J. Bonnet,et al.  Detection of Mycoplasma pneumoniae by using the polymerase chain reaction , 1989 .

[105]  P. Klatser,et al.  Polymerase chain reaction for the detection of Mycobacterium leprae. , 1989, Journal of general microbiology.

[106]  D. M. Olive,et al.  Detection of enterotoxigenic Escherichia coli after polymerase chain reaction amplification with a thermostable DNA polymerase , 1989, Journal of clinical microbiology.

[107]  D. M. Olive,et al.  Detection of toxigenic Escherichia coli using biotin-labelled DNA probes following enzymatic amplification of the heat labile toxin gene. , 1988, Molecular and cellular probes.

[108]  D. Graves,et al.  Ethidium bromide and its photoreactive analogues: spectroscopic analysis of deoxyribonucleic acid binding properties. , 1981, Biochemistry.

[109]  J. Knowles.  Photogenerated reagents for biological receptor-site labeling , 1972 .

[110]  A. Nocker,et al.  Optimization of viability qPCR for selective detection of membrane-intact Legionella pneumophila. , 2019, Journal of microbiological methods.

[111]  B. Örmeci,et al.  Development of a sensitive and false-positive free PMA-qPCR viability assay to quantify VBNC Escherichia coli and evaluate disinfection performance in wastewater effluent. , 2017, Journal of microbiological methods.

[112]  Alison M. Cupples,et al.  Most probable number - loop mediated isothermal amplification (MPN-LAMP) for quantifying waterborne pathogens in <25min. , 2017, Journal of microbiological methods.

[113]  A. Nocker,et al.  Discrimination between live and dead cellsin bacterial communities from environmental water samples analyzed by 454 pyrosequencing. , 2010, International microbiology : the official journal of the Spanish Society for Microbiology.

[114]  A. Camper,et al.  Viable Real-Time PCR in Environmental Samples: Can All Data Be Interpreted Directly? , 2010, Microbial Ecology.

[115]  Ben-Jye Chang,et al.  Vehicle Location and Navigation Systems , 2010 .

[116]  R. Levin,et al.  A comparative study of the ability of EMA and PMA to distinguish viable from heat killed mixed bacterial flora from fish fillets. , 2009, Journal of microbiological methods.

[117]  M R Barer,et al.  Bacterial viability and culturability. , 1999, Advances in microbial physiology.

[118]  C. Stewart,et al.  Use of a photolabeling technique to identify nonviable cells in fixed homologous or heterologous cell populations. , 1991, Cytometry.

[119]  A. Vekris,et al.  Specific amplification of a DNA sequence common to all Chlamydia trachomatis serovars using the polymerase chain reaction. , 1989, Research in microbiology.