Urinary excretion of lactose and oligosaccharides in preterm infants fed human milk or infant formula

At present, not much is known about the absorption and metabolism of human milk (HM) oligosaccharides in term and preterm infants. We investigated the renal excretion of lactose and complex oligosaccharides in preterm infants fed HM (n= 9, mean actual body weight 2290 g) or a cow's milk‐based infant formula (n= 9, mean actual body weight 2470 g). We found that the renal excretion of lactose in HM‐fed infants was slightly lower than in formula‐fed infants (14.0 ± 7.4 versus 20.4 ± 8.7 mg kg‐1 day‐1, mean ± SD). The excretion of neutral sugars deriving from oligosaccharides was similar in HM‐fed and formula‐fed infants (3.8 ± 2.1 versus 2.9 ± 0.9mgkg‐1 day1‐); the difference between means was not statistically significant. The separation and characterization of oligosaccharides by high‐pH anion exchange chromatography with pulsed amperometric detection (HPAE‐PAD) and subsequent analysis by fast atom bombardment‐mass spectrometry (FAB‐MS) revealed a more complex pattern in HM‐fed infants compared to the formula‐fed group. Lactose‐derived oligosaccharides characteristic for HM (e.g lacto‐N‐tetraose, and lacto‐N‐fucopentaoses I and II) were excreted in HM‐fed but not in formula‐fed infants. These results indicate that nutrition has a significant impact on the oligosaccharide composition in urine of preterm infants.

[1]  E. Savilahti,et al.  Human α-Lactalbumin and Bovine β-Lactoglobulin Absorption in Premature Infants , 1994, Pediatric Research.

[2]  J. Marks,et al.  Review of the epidemiologic evidence for an association between infant feeding and infant health. , 1984, Pediatrics.

[3]  S. Mårild,et al.  Breastfeeding and urinary-tract infection , 1990, The Lancet.

[4]  R. Goldblum,et al.  Human Milk Feeding Enhances the Urinary Excretion of Immunologic Factors in Low Birth Weight Infants , 1989, Pediatric Research.

[5]  A. Cravioto,et al.  Inhibition of localized adhesion of enteropathogenic Escherichia coli to HEp-2 cells by immunoglobulin and oligosaccharide fractions of human colostrum and breast milk. , 1991, The Journal of infectious diseases.

[6]  B. Stoll,et al.  Protection against cholera in breast-fed children by antibodies in breast milk. , 1983, The New England journal of medicine.

[7]  J. Marks,et al.  Mortality and infectious disease associated with infant-feeding practices in developing countries. , 1984, Pediatrics.

[8]  T. Yip,et al.  Structurally intact (78-kDa) forms of maternal lactoferrin purified from urine of preterm infants fed human milk: identification of a trypsin-like proteolytic cleavage event in vivo that does not result in fragment dissociation. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[9]  J. Peter-Katalinic,et al.  Fast atom bombardment mass spectrometry for structural elucidation of glycoconjugates , 1987 .

[10]  A. Lundblad,et al.  Sialylated oligosaccharides in human milk and feces of preterm, full-term, and weaning infants. , 1991, Journal of pediatric gastroenterology and nutrition.

[11]  A. Dell,et al.  Fucose containing oligosaccharides from human milk. I. Separation and identification of new constituents. , 1983, Archives of biochemistry and biophysics.

[12]  A. Lundblad,et al.  Oligosaccharides from feces of preterm infants fed on breast milk. , 1988, Archives of biochemistry and biophysics.

[13]  J. Holmgren,et al.  Receptor-like glycocompounds in human milk that inhibit classical and El Tor Vibrio cholerae cell adherence (hemagglutination) , 1983, Infection and immunity.

[14]  S. Polberger,et al.  Macromolecular Absorption in Preterm and Term Infants , 1989, Acta paediatrica Scandinavica.

[15]  E. Hounsell,et al.  NEW TYPE OF ADHESIVE SPECIFICITY REVEALED BY OLIGOSACCHARIDE PROBES IN ESCHERICHIA COLI FROM PATIENTS WITH URINARY TRACT INFECTION , 1988, The Lancet.

[16]  C. Kunz,et al.  Biological functions of oligosaccharides in human milk , 1993, Acta paediatrica.

[17]  C. Svanborg,et al.  Intestinal Colonization with Enterobacteriaceae in Pakistani and Swedish Hospital‐Delivered Infants , 1991, Acta paediatrica Scandinavica.

[18]  P. Burrows,et al.  Simultaneous assessment of intestinal permeability and lactose tolerance with orally administered raffinose, lactose and L-arabinose. , 1990, Clinical science.

[19]  A. Prentice,et al.  Breast feeding increases concentrations of IgA in infants' urine. , 1987, Archives of disease in childhood.

[20]  L. Svennerholm,et al.  Quantitative estimation of sialic acids. II. A colorimetric resorcinol-hydrochloric acid method. , 1957, Biochimica et Biophysica Acta.

[21]  A. Pisacane,et al.  Breast-feeding and urinary tract infection. , 1992, The Journal of pediatrics.

[22]  L. Hanson,et al.  NON-ANTIBODY-CONTAINING FRACTIONS OF BREAST MILK INHIBIT EPITHELIAL ATTACHMENT OF STREPTOCOCCUS PNEUMONIAE AND HAEMOPHILUS INFLUENZAE , 1985, The Lancet.

[23]  S. Ahlstedt,et al.  Protective factors in milk and the development of the immune system. , 1985, Pediatrics.

[24]  R. Goldblum,et al.  Molecular Forms of Lactoferrin in Stool and Urine from Infants Fed Human Milk , 1990, Pediatric Research.