EFFECT OF ERYTHROPOIETIN ON PROLIFERATION OF ERYTHROPOIETIN‐RESPONSIVE CELLS

A single dose of Myleran suppressed CFU in polycythemic mice to around 1% of normal for a period of 2 weeks and permitted the study of effects of erythropoietin on unipotential, erythroid stem cells (erythropoietin‐responsive cells, ERC) in the absence of cell inflow from the CFU compartment. Without erythropoietin no ERC were detectable for 12 days after Myleran. Injections of erythropoietin had no effect on CFU but restored ERC populations in proportion to the dose of erythropoietin. Hydroxyurea given after erythropoietin markedly inhibited ERC repopulation and the latter is attributed to a stimulation of ERC proliferation by erythropoietin. Evidence in support of an age structure in the ERC population is presented. Daily erythropoietin injection resulted in stable ERC populations, indicating that ERC loss through differentiation and ERC self‐replication were in balance.

[1]  K. Udupa,et al.  Homogeneity of Erythropoietin in Its Effect on Self-Replication and Differentiation of Erythropoietin-Responsive Cells 1 , 1972, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[2]  K. Udupa,et al.  Granulopoiesis during myleran-induced suppression of transplantable hen atopoietic stem cells. , 1972, Blood.

[3]  L. Lajtha,et al.  Kinetics of Haemopoietic Colony Growth , 1971, British journal of haematology.

[4]  L. O'Grady,et al.  Proliferation of erythroid-committed cells in the absence of erythropoietin. , 1970, The Journal of laboratory and clinical medicine.

[5]  K. Reissmann,et al.  Effect of erythropoietin on proliferation of erythroid stem cells in the absence of transplantable colony-forming units. , 1970, Blood.

[6]  N. Rencricca,et al.  Relationship of erythropoietin effectiveness to the generative cycle of erythroid precursor cell. , 1970, Blood.

[7]  L. A. Elson,et al.  THE COMPARATIVE EFFECT OF BUSULPHAN (‘MYLERAN’) AND AMINOCHLORAMBUCIL ON HAEMOPOIETIC COLONY FORMING UNITS IN THE RAT , 1970, Cell and tissue kinetics.

[8]  L. Lajtha,et al.  KINETIC PROPERTIES OF HAEMOPOIETIC STEM CELLS , 1969 .

[9]  N. Blackett.  Investigation of bone-marrow stem cell proliferation in normal, anemic, and irradiated rats, using methotrexate and tritiated thymidine. , 1968, Journal of the National Cancer Institute.

[10]  I. Hanna.  Response of Early Erythroid Precursors to Bleeding , 1967, Nature.

[11]  W. K. Sinclair.  Hydroxyurea: effects on Chinese hamster cells grown in culture. , 1967, Cancer research.

[12]  K. Reissmann,et al.  INFLUENCE OF DISAPPEARANCE RATE AND DISTRIBUTION SPACE ON PLASMA CONCENTRATION OF ERYTHROPOIETIN IN NORMAL RATS. , 1965, The Journal of laboratory and clinical medicine.

[13]  L. Lajtha,et al.  A Method for Investigation of Stern‐Cell Kinetics , 1962 .

[14]  L. Lajtha,et al.  A method for investigation of stem-cell kinetics. , 1962, British journal of haematology.

[15]  J. Till,et al.  The radiation sensitivity of normal mouse bone marrow cells, determined by quantitative marrow transplantation into irradiated mice. , 1960, Radiation Research.

[16]  L. A. Elson,et al.  The Action of Chlorambucil (CB. 1348) and Busulphan (Myleran) on the Haemopoietic Organs of the Rat , 1958, British journal of haematology.