Reply to: COVID-19 and Coagulopathy

1. Suprynowicz FA, Upadhyay G, Krawczyk E, Kramer SC, Hebert JD, Liu X, et al. Conditionally reprogrammed cells represent a stem-like state of adult epithelial cells. Proc Natl Acad Sci USA 2012;109:20035–20040. 2. Liu X, Ory V, Chapman S, Yuan H, Albanese C, Kallakury B, et al. ROCK inhibitor and feeder cells induce the conditional reprogramming of epithelial cells. Am J Pathol 2012;180:599–607. 3. Koh KD, Siddiqui S, Cheng D, Bonser LR, Sun DI, Zlock LT, et al. Efficient RNP-directed human gene targeting reveals SPDEF is required for IL13-induced mucostasis. Am J Respir Cell Mol Biol 2020;62:373–381. 4. Rapiteanu R, Karagyozova T, Zimmermann N, Singh K, Wayne G, Martufi M, et al. Highly efficient genome editing in primary human bronchial epithelial cells differentiated at air-liquid interface. Eur Respir J 2020;55:1900950. 5. Ranney ML, Griffeth V, Jha AK. Critical supply shortages the need for ventilators and personal protective equipment during the covid-19 pandemic. N Engl J Med 2020;382:e41. 6. Bell CL, Quinton PM. Recycle those cell culture inserts. In Vitro Cell Dev Biol 1990;26:1123–1124. 7. Fulcher ML, Randell SH. Human nasal and tracheo-bronchial respiratory epithelial cell culture. Methods Mol Biol 2013;945:109–121. 8. Chen G, Sun L, Kato T, Okuda K, Martino MB, Abzhanova A, et al. IL-1b dominates the promucin secretory cytokine profile in cystic fibrosis. J Clin Invest 2019;129:4433–4450. 9. Grubb BR, Rogers TD, Diggs PC, Boucher RC, Ostrowski LE. Culture of murine nasal epithelia: model for cystic fibrosis. Am J Physiol Lung Cell Mol Physiol 2006;290:L270–L277. 10. Sisson JH, Stoner JA, Ammons BA, Wyatt TA. All-digital image capture and whole-field analysis of ciliary beat frequency. J Microsc 2003;211:103–111. 11. Ostrowski LE, Yin W, Patel M, Sechelski J, Rogers T, Burns K, et al. Restoring ciliary function to differentiated primary ciliary dyskinesia cells with a lentiviral vector. Gene Ther 2014;21:253–261. 12. Hsiau T, Conant D, Rossi N, Maures T, Waite K, Yang J, et al. Inference of CRISPR edits from Sanger trace data [preprint]. bioRxiv; 2019 [accessed 2020 Apr 4]. Available from: https://www.biorxiv.org/content/ 10.1101/251082v3.

[1]  V. Peinado,et al.  Pulmonary Endothelial Dysfunction and Thrombotic Complications in Patients with COVID-19 , 2020, American journal of respiratory cell and molecular biology.

[2]  M. Drutskaya,et al.  IL-6: Relevance for immunopathology of SARS-CoV-2 , 2020, Cytokine & Growth Factor Reviews.

[3]  Suxin Wan,et al.  Characteristics of lymphocyte subsets and cytokines in peripheral blood of 123 hospitalized patients with 2019 novel coronavirus pneumonia (NCP) , 2020, medRxiv.

[4]  N. Mukaida,et al.  Crucial Involvement of IL-6 in Thrombus Resolution in Mice via Macrophage Recruitment and the Induction of Proteolytic Enzymes , 2020, Frontiers in Immunology.

[5]  J. Maher,et al.  The role of the interleukin (IL)-6/IL-6 receptor axis in cancer. , 2018, Biochemical Society transactions.

[6]  S. Reddy,et al.  Sirtuin 1 Promotes Hyperoxia-Induced Lung Epithelial Cell Death Independent of NF-E2-Related Factor 2 Activation. , 2016, American journal of respiratory cell and molecular biology.

[7]  E. Senchenkova,et al.  Interleukin-6 mediates the platelet abnormalities and thrombogenesis associated with experimental colitis. , 2013, The American journal of pathology.

[8]  T. Yoshikawa,et al.  Role of interleukin-6 in fibrinolytic changes induced by lipopolysaccharide in mice , 2006, Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis.

[9]  C. Tohyama,et al.  Protective role of interleukin-6 in coagulatory and hemostatic disturbance induced by lipopolysaccharide in mice , 2004, Thrombosis and Haemostasis.

[10]  M. Jordana,et al.  IL-6 is an antiinflammatory cytokine required for controlling local or systemic acute inflammatory responses. , 1998, The Journal of clinical investigation.