Hydrogen supersaturation in thermophilic mixed culture fermentation

Abstract Hydrogen concentration is important for the metabolic distribution in mixed culture fermentation (MCF) but hydrogen supersaturation is often ignored. In this study, hydrogen supersaturation in thermophilic MCF was investigated online by a membrane inlet mass spectrometry. The results showed that with the increase of glucose loading rate (from 13.5 to 137.5 mmol/L/d) and the decrease of Reynolds number (from 12,900 to 3500), the hydrogen partial pressure ( P H 2 ) remained almost unchanged, but the hydrogen concentration in liquid (H2aq) increased from 0.82 to 1.27 and from 0.68 to 1.21 mmol/L, respectively. It demonstrated that hydrogen supersaturation occurred and the supersaturation ratio was between 1.7 and 3.0. Meanwhile, higher H2aq resulted in lower hydrogen yield, lower glucose degradation rate and higher mole ratio of ethanol/(acetate + butyrate). Thus, H2aq is more appropriate than P H 2 when discussing the H2 role in MCF. Furthermore, the calculated KLa clearly illustrated that the required KLa values for maintaining low H2aq were order of magnitudes higher than the experimental ones. Therefore, hydrogen supersaturation is inevitable in practice and should be considered in MCF.

[1]  J. Steyer,et al.  Development of membrane inlet mass spectrometry for examination of fermentation processes. , 2010, Talanta.

[2]  A. E. Greenberg,et al.  Standard methods for the examination of water and wastewater : supplement to the sixteenth edition , 1988 .

[3]  P. H. Hemberger,et al.  Membrane introduction mass spectrometry: trends and applications. , 2000, Mass spectrometry reviews.

[4]  D. Bagley,et al.  Measurement of H2 consumption and its role in continuous fermentative hydrogen production. , 2008, Water science and technology : a journal of the International Association on Water Pollution Research.

[5]  A. Stams,et al.  Substrate and product inhibition of hydrogen production by the extreme thermophile, Caldicellulosiruptor saccharolyticus. , 2003, Biotechnology and bioengineering.

[6]  Jean-Philippe Steyer,et al.  Experimental determination by principal component analysis of a reaction pathway of biohydrogen production by anaerobic fermentation , 2008 .

[7]  Don W. Green,et al.  Perry's Chemical Engineers' Handbook , 2007 .

[8]  Robbert Kleerebezem,et al.  Influence of the pH on (open) mixed culture fermentation of glucose: A chemostat study , 2007, Biotechnology and bioengineering.

[9]  Irini Angelidaki,et al.  Biohydrogen production in granular up‐flow anaerobic sludge blanket (UASB) reactors with mixed cultures under hyper‐thermophilic temperature (70°C) , 2006, Biotechnology and bioengineering.

[10]  Hang-Sik Shin,et al.  Effect of substrate concentration on hydrogen production and 16S rDNA-based analysis of the microbial community in a continuous fermenter , 2006 .

[11]  J. Charpentier,et al.  Mass-Transfer Rates in Gas-Liquid Absorbers and Reactors , 1981 .

[12]  G. Zacchi,et al.  A kinetic model for quantitative evaluation of the effect of hydrogen and osmolarity on hydrogen production by Caldicellulosiruptor saccharolyticus , 2011, Biotechnology for biofuels.

[13]  Jyeshtharaj B. Joshi,et al.  Role of hydrodynamic shear in the cultivation of animal, plant and microbial cells , 1996 .

[14]  D. Bagley,et al.  Optimisation and design of nitrogen-sparged fermentative hydrogen production bioreactors , 2008 .

[15]  I. Darah,et al.  Influence of Agitation Speed on Tannase Production and Morphology of Aspergillus niger FETL FT3 in Submerged Fermentation , 2011, Applied biochemistry and biotechnology.

[16]  H. Siegrist,et al.  The IWA Anaerobic Digestion Model No 1 (ADM1). , 2002, Water science and technology : a journal of the International Association on Water Pollution Research.

[17]  D. Bagley,et al.  Supersaturation of Dissolved H2 and CO2 During Fermentative Hydrogen Production with N2 Sparging , 2006, Biotechnology Letters.

[18]  M Perrier,et al.  Liquid-to-Gas Mass Transfer in Anaerobic Processes: Inevitable Transfer Limitations of Methane and Hydrogen in the Biomethanation Process , 1990, Applied and environmental microbiology.

[19]  R. Hedderich,et al.  A multisubunit membrane-bound [NiFe] hydrogenase and an NADH-dependent Fe-only hydrogenase in the fermenting bacterium Thermoanaerobacter tengcongensis. , 2004, Microbiology.

[20]  B. Logan,et al.  Increased biological hydrogen production with reduced organic loading. , 2005, Water research.

[21]  R. Lamed,et al.  Effects of Stirring and Hydrogen on Fermentation Products of Clostridium thermocellum , 1988, Applied and environmental microbiology.

[22]  F. Smith,et al.  COLORIMETRIC METHOD FOR DETER-MINATION OF SUGAR AND RELATED SUBSTANCE , 1956 .

[23]  Lawrence Pitt,et al.  Biohydrogen production: prospects and limitations to practical application , 2004 .

[24]  Hanqing Yu,et al.  Effects of temperature and substrate concentration on biological hydrogen production from starch , 2009 .

[25]  Han-Qing Yu,et al.  Comparative performance of mesophilic and thermophilic acidogenic upflow reactors , 2002 .

[26]  M. Shih,et al.  Mixed culture fermentation from lignocellulosic materials using thermophilic lignocellulose-degrading anaerobes , 2011 .

[27]  Hassib Bouallagui,et al.  Mesophilic and thermophilic anaerobic co-digestion of olive mill wastewaters and abattoir wastewaters in an upflow anaerobic filter , 2007 .

[28]  J. Kestin,et al.  Viscosity of Liquid Water in the Range - 8 C to 150 C, , 1978 .

[29]  L. T. Angenent,et al.  Production of bioenergy and biochemicals from industrial and agricultural wastewater. , 2004, Trends in biotechnology.

[30]  A. E. Greenberg,et al.  Standard methods for the examination of water and wastewater. 19th ed. 1995. , 1995 .