Development of a membraneless ethanol/oxygen biofuel cell

Abstract Biofuel cells are similar to traditional fuel cells, except the metallic electrocatalyst is replaced with a biological electrocatalyst. This paper details the development of an enzymatic biofuel cell, which employs alcohol dehydrogenase to oxidize ethanol at the anode and bilirubin oxidase to reduce oxygen at the cathode. This ethanol/oxygen biofuel cell has an active lifetime of about 30 days and shows power densities of up to 0.46 mW/cm 2 . The biocathode described in this paper is unique in that bilirubin oxidase is immobilized within a modified Nafion polymer that acts both to entrap and stabilize the enzyme, while also containing the redox mediator in concentrations large enough for self-exchange based conduction of electrons between the enzyme and the electrode. This biocathode is fuel tolerant, which leads to a unique fuel cell that employs both renewable catalysts and fuel, but does not require a separator membrane to separate anolyte from catholyte.

[1]  Shaojun Dong,et al.  Electrogenerated chemiluminescence from R(bpy)3(2+) ion-exchanged in carbon nanotube/perfluorosulfonated ionomer composite films. , 2004, Analytical chemistry.

[2]  G. Gil,et al.  Operational parameters affecting the performannce of a mediator-less microbial fuel cell. , 2003, Biosensors & bioelectronics.

[3]  John D. Enderle,et al.  Introduction to Biomedical Engineering , 1999 .

[4]  Weijiang Zhou,et al.  Performance comparison of low-temperature direct alcohol fuel cells with different anode catalysts , 2004 .

[5]  N. Mano,et al.  Characteristics of a miniature compartment-less glucose-O2 biofuel cell and its operation in a living plant. , 2003, Journal of the American Chemical Society.

[6]  D. Lovley,et al.  Electricity generation by direct oxidation of glucose in mediatorless microbial fuel cells , 2003, Nature Biotechnology.

[7]  A Martinez,et al.  Enteric Bacterial Catalysts for Fuel Ethanol Production , 1999, Biotechnology progress.

[8]  H. Görisch.  The ethanol oxidation system and its regulation in Pseudomonas aeruginosa. , 2003, Biochimica et biophysica acta.

[9]  Adam Heller,et al.  A miniature biofuel cell operating in a physiological buffer. , 2002, Journal of the American Chemical Society.

[10]  Itamar Willner,et al.  A biofuel cell with electrochemically switchable and tunable power output. , 2003, Journal of the American Chemical Society.

[11]  George M. Whitesides,et al.  A methanol/dioxygen biofuel cell that uses NAD+-dependent dehydrogenases as catalysts: application of an electro-enzymatic method to regenerate nicotinamide adenine dinucleotide at low overpotentials , 1998 .

[12]  Nick L. Akers,et al.  Improving the environment for immobilized dehydrogenase enzymes by modifying Nafion with tetraalkylammonium bromides. , 2004, Biomacromolecules.

[13]  P. Kenis,et al.  Membraneless Fuel Cell Based on Laminar Flow , 2003 .

[14]  F. Molinari,et al.  Aldehyde production by alcohol oxidation with Gluconobacter oxydans , 1995, Applied Microbiology and Biotechnology.

[15]  Hong Liu,et al.  Production of electricity during wastewater treatment using a single chamber microbial fuel cell. , 2004, Environmental science & technology.

[16]  W. Verstraete,et al.  A microbial fuel cell capable of converting glucose to electricity at high rate and efficiency , 2004, Biotechnology Letters.

[17]  Johna Leddy,et al.  Density of nafion exchanged with transition metal complexes and tetramethyl ammonium, ferrous, and hydrogen ions: commercial and recast films. , 2002, Analytical chemistry.

[18]  Byung Hong Kim,et al.  Novel BOD (biological oxygen demand) sensor using mediator-less microbial fuel cell , 2003, Biotechnology Letters.

[19]  Collinson,et al.  Electrogenerated chemiluminescence of tris (2,2'-bipyridyl)ruthenium(II) ion-exchanged in nafion-silica composite films , 2000, Analytical chemistry.

[20]  Larry J. Markoski,et al.  Microfluidic fuel cell based on laminar flow , 2004 .

[21]  V. Daniel Hunt.  The Gasohol Handbook , 1981 .

[22]  D. R. Bond,et al.  Electricity Production by Geobacter sulfurreducens Attached to Electrodes , 2003, Applied and Environmental Microbiology.

[23]  K. Kano,et al.  Mediated catalytic current for the oxidation of ethanol produced by Acetobacter aceti cells suspended in solution , 1997 .

[24]  Weijiang Zhou.  Pt based anode catalysts for direct ethanol fuel cells , 2003 .

[25]  Adam Heller,et al.  A miniature biofuel cell operating at 0.78 V. , 2003, Chemical communications.

[26]  Hong Liu,et al.  Electricity generation using an air-cathode single chamber microbial fuel cell in the presence and absence of a proton exchange membrane. , 2004, Environmental science & technology.