Generation of HIV-1 derivatives that productively infect macaque monkey lymphoid cells

The narrow host range of human immunodeficiency virus type 1 (HIV-1) is caused in part by innate cellular factors such as apolipoprotein B mRNA-editing enzyme-catalytic polypeptide-like 3G (APOBEC3G) and TRIM5α, which restrict virus replication in monkey cells. Variant HIV-1 molecular clones containing both a 21-nucleotide simian immunodeficiency virus (SIV) Gag CA element, corresponding to the HIV-1 cyclophilin A-binding site, and the entire SIV vif gene were constructed. Long-term passage in a cynomolgus monkey lymphoid cell line resulted in the acquisition of two nonsynonymous changes in env, which conferred improved replication properties. A proviral molecular clone, derived from infected cells and designated NL-DT5R, was used to generate virus stocks capable of establishing spreading infections in the cynomolgus monkey T cell line and CD8-depleted peripheral blood mononuclear cells from five of five pig-tailed macaques and one of three rhesus monkeys. NL-DT5R, which genetically is >93% HIV-1, provides the opportunity, not possible with currently available SIV/HIV chimeric viruses, to analyze the function of multiple HIV-1 genes in a broad range of nonhuman primate species.

[1]  G. Manning,et al.  Mutational Alteration of Human Immunodeficiency Virus Type 1 Vif Allows for Functional Interaction with Nonhuman Primate APOBEC3G , 2006, Journal of Virology.

[2]  G. Towers,et al.  Cyclophilin A Renders Human Immunodeficiency Virus Type 1 Sensitive to Old World Monkey but Not Human TRIM5α Antiviral Activity , 2006, Journal of Virology.

[3]  Joseph Sodroski,et al.  Specific recognition and accelerated uncoating of retroviral capsids by the TRIM5alpha restriction factor. , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[4]  J. Luban,et al.  Cyclophilin A is required for TRIM5α-mediated resistance to HIV-1 in Old World monkey cells , 2005 .

[5]  J. Sodroski,et al.  TRIM5alpha mediates the postentry block to N-tropic murine leukemia viruses in human cells. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[6]  A. Yang,et al.  Retrovirus resistance factors Ref1 and Lv1 are species-specific variants of TRIM5alpha. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[7]  A. Sakurai,et al.  Functional analysis of HIV-1 vif genes derived from Japanese long-term nonprogressors and progressors for AIDS. , 2004, Microbes and infection.

[8]  K. Kamada,et al.  Transcriptional regulation of TT virus: promoter and enhancer regions in the 1.2-kb noncoding region. , 2004, Virology.

[9]  C. M. Owens,et al.  The cytoplasmic body component TRIM5α restricts HIV-1 infection in Old World monkeys , 2004, Nature.

[10]  Yunkai Yu,et al.  Induction of APOBEC3G Ubiquitination and Degradation by an HIV-1 Vif-Cul5-SCF Complex , 2003, Science.

[11]  M. Khan,et al.  The human immunodeficiency virus type 1 Vif protein reduces intracellular expression and inhibits packaging of APOBEC3G (CEM15), a cellular inhibitor of virus infectivity. , 2003 .

[12]  M. Malim,et al.  The antiretroviral enzyme APOBEC3G is degraded by the proteasome in response to HIV-1 Vif , 2003, Nature Medicine.

[13]  M. Marin,et al.  HIV-1 Vif protein binds the editing enzyme APOBEC3G and induces its degradation , 2003, Nature Medicine.

[14]  J. Mascola,et al.  Cellular Immunity Elicited by Human Immunodeficiency Virus Type 1/ Simian Immunodeficiency Virus DNA Vaccination Does Not Augment the Sterile Protection Afforded by Passive Infusion of Neutralizing Antibodies , 2003, Journal of Virology.

[15]  P. Bieniasz,et al.  Cyclophilin A modulates the sensitivity of HIV-1 to host restriction factors , 2003, Nature Medicine.

[16]  R. König,et al.  Species-Specific Exclusion of APOBEC3G from HIV-1 Virions by Vif , 2003, Cell.

[17]  Gersende Caron,et al.  Broad antiretroviral defence by human APOBEC3G through lethal editing of nascent reverse transcripts , 2003, Nature.

[18]  F. Clavel,et al.  Hypermutation of HIV-1 DNA in the Absence of the Vif Protein , 2003, Science.

[19]  H. Lane,et al.  Amino acid deletions are introduced into the V2 region of gp120 during independent pathogenic simian immunodeficiency virus/HIV chimeric virus (SHIV) infections of rhesus monkeys generating variants that are macrophage tropic , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[20]  M. Malim,et al.  Isolation of a human gene that inhibits HIV-1 infection and is suppressed by the viral Vif protein , 2002, Nature.

[21]  N. Haigwood,et al.  Determination of a Statistically Valid Neutralization Titer in Plasma That Confers Protection against Simian-Human Immunodeficiency Virus Challenge following Passive Transfer of High-Titered Neutralizing Antibodies , 2002, Journal of Virology.

[22]  A. Sakurai,et al.  Cyclophilin A-Independent Replication of a Human Immunodeficiency Virus Type 1 Isolate Carrying a Small Portion of the Simian Immunodeficiency Virus SIVMACgag Capsid Region , 2001, Journal of Virology.

[23]  James G. Herndon,et al.  Control of a Mucosal Challenge and Prevention of AIDS by a Multiprotein DNA/MVA Vaccine , 2001, Science.

[24]  M. Malim,et al.  Human Immunodeficiency Virus Type 1 Spinoculation Enhances Infection through Virus Binding , 2000, Journal of Virology.

[25]  H. Akari,et al.  Induction of apoptosis in Herpesvirus saimiri-immortalized T lymphocytes by blocking interaction of CD28 with CD80/CD86. , 1999, Biochemical and biophysical research communications.

[26]  J. Sodroski,et al.  Characterization of molecularly cloned simian-human immunodeficiency viruses causing rapid CD4+ lymphocyte depletion in rhesus monkeys , 1997, Journal of virology.

[27]  J. Sodroski,et al.  An env gene derived from a primary human immunodeficiency virus type 1 isolate confers high in vivo replicative capacity to a chimeric simian/human immunodeficiency virus in rhesus monkeys , 1996, Journal of virology.

[28]  H. McClure,et al.  Chimeric simian/human immunodeficiency virus that causes progressive loss of CD4+ T cells and AIDS in pig-tailed macaques , 1996, Journal of virology.

[29]  E. Freed,et al.  Domains of the human immunodeficiency virus type 1 matrix and gp41 cytoplasmic tail required for envelope incorporation into virions , 1996, Journal of virology.

[30]  J. Sodroski,et al.  T cell receptor V beta repertoire in an acute infection of rhesus monkeys with simian immunodeficiency viruses and a chimeric simian- human immunodeficiency virus , 1995, The Journal of experimental medicine.

[31]  J. Burns,et al.  A general method for the generation of high-titer, pantropic retroviral vectors: highly efficient infection of primary hepatocytes. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[32]  Jeremy Luban,et al.  Human immunodeficiency virus type 1 Gag protein binds to cyclophilins A and B , 1993, Cell.

[33]  M. Emerman,et al.  Detection of replication-competent and pseudotyped human immunodeficiency virus with a sensitive cell line on the basis of activation of an integrated beta-galactosidase gene , 1992, Journal of virology.

[34]  A. Ishimoto,et al.  Generation of a chimeric human and simian immunodeficiency virus infectious to monkey peripheral blood mononuclear cells , 1991, Journal of virology.

[35]  P. Earl,et al.  In vitro mutagenesis identifies a region within the envelope gene of the human immunodeficiency virus that is critical for infectivity , 1988, Journal of virology.

[36]  H. Gendelman,et al.  Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone , 1986, Journal of virology.