Effect of Dual Inoculation of Arbuscular Mycorrhiza and Rhizobium on the Chlorophyll, Nitrogen and Phosphorus Contents of Pigeon Pea (Cajanus cajan L.)

The pot experiments were conducted in the Department of Life Science and Bioinformatics of Assam University, Silchar. The aim of the present study was to investigate the effect of dual inoculation of Arbuscular mycorrhiza (AM) (Glomus fasciculatum) and Rhizobium on the chlorophyll, nitrogen and phosphorus contents of pigeon pea (Cajanus cajan L.). The results revealed an overall increase in chlorophyll, nitrogen and phosphorus contents in the inoculated plants than uninoculated ones. Maximum chlorophyll contents were recorded in the plants dually inoculated with Glomus fasciculatum and Rhizobium. The combined application of Glomus fasciculatum and Rhizobium also remarkably increased the nitrogen and phosphorus contents of pigeon pea. The dual inoculation with microsymbionts revealed synergistic effect. The results suggest that dual inoculation of Glomus fasciculatum and Rhizobium have the potential to enhance the chlorophyll, nitrogen and phosphorus contents of pigeon pea.

[1]  K. Krishna,et al.  Growth and nutrient uptake of peanut inoculated with the mycorrhizal fungusGlomus fasciculatum compared with non-inoculated ones , 1984, Plant and Soil.

[2]  B. S. Dwivedi,et al.  BIOFERTILIZERS FOR CEREAL PRODUCTION IN INDIA : A REVIEW , 1999 .

[3]  M. Mardi,et al.  Interactive effects of Arbuscular mycorrhizal fungi and rhizobial strains on chickpea growth and nutrient content in plant , 2011 .

[4]  D. Swelim,et al.  Improving salinity tolerance of Acacia saligna (Labill.) plant by arbuscular mycorrhizal fungi and Rhizobium inoculation , 2012 .

[5]  G. Abdel-Fattah,et al.  Interactions between a vesicular-arbuscular mycorrhizal fungus (Glomus intraradices) and Streptomyces coelicolor and their effects on sorghum plants grown in soil amended with chitin of brawn scales , 2000, Biology and Fertility of Soils.

[6]  R. Bravo,et al.  COLONIZACIÓN MICORRÍZICA ARBUSCULAR Y CRECIMIENTO DE GENOTIPOS DE PASTO BUFFEL (Cenchrus ciliaris) ARBUSCULAR MYCORRHIZAL COLONIZATION AND GROWTH OF BUFFEL GRASS (Cenchrus ciliaris) GENOTYPES , 2006 .

[7]  J. Bai,et al.  The influence of arbuscular mycorrhizal fungi on As and P uptake by maize (Zea mays L.) from As-contaminated soils , 2008 .

[8]  J. Vincent A manual for the practical study of root-nodule bacteria , 1971 .

[9]  T. Nicolson,et al.  Spores of mycorrhizal Endogone species extracted from soil by wet sieving and decanting , 1963 .

[10]  Y. D. Gaur,et al.  Cultural and Biochemical Characteristics of Root Nodule Bacteria of Chickpea (Cicer arietinum L.) , 1981 .

[11]  R. Clárk,et al.  Mineral acquisition by arbuscular mycorrhizal plants , 2000 .

[12]  K. Minamisawa,et al.  Plant-microbe communications for symbiosis. , 2010, Plant & cell physiology.

[13]  E. Cardoso,et al.  Arbuscular mycorrhiza and kinetic parameters of phosphorus absorption by bean plants , 2004 .

[14]  M. H. Abd‐Alla,et al.  SURVIVAL OF RHIZOBIA/BRADYRHIZOBIA AND A ROCK-PHOSPHATE-SOLUBILIZING FUNGUS ASPERGILLUS NIGER ON VARIOUS CARRIERS FROM SOME AGRO-INDUSTRIAL WASTES AND THEIR EFFECTS ON NODULATION AND GROWTH OF FABA BEAN AND SOYBEAN , 2001 .

[15]  J. Vessey Plant growth promoting rhizobacteria as biofertilizers , 2003, Plant and Soil.

[16]  B. Lugtenberg,et al.  Plant-growth-promoting rhizobacteria. , 2009, Annual review of microbiology.

[17]  N. B. Talaat,et al.  Response of Faba Bean (Vicia faba L.) to Dual Inoculation with Rhizobium and VA Mycorrhiza under Different Levels of N and P Fertilization , 2008 .

[18]  R. H. Bray,et al.  DETERMINATION OF TOTAL, ORGANIC, AND AVAILABLE FORMS OF PHOSPHORUS IN SOILS , 1945 .

[19]  N. Rao,et al.  Synergistic effect of vesicular-arbuscular mycorrhizas and Azospirillum brasilense on the growth of barley in pots , 1985 .

[20]  V. G. Panse,et al.  Statistical methods for agricultural workers. , 1954 .

[21]  W. Schwartz J. M. Vincent, A Manual for the Practical Study of the Root-Nodule Bacteria (IBP Handbuch No. 15 des International Biology Program, London). XI u. 164 S., 10 Abb., 17 Tab., 7 Taf. Oxford-Edinburgh 1970: Blackwell Scientific Publ., 45 s , 1972 .