Low-intensity pulsed ultrasound-mediated stimulation of hematopoietic stem/progenitor cell viability, proliferation and differentiation in vitro

Low-intensity pulsed ultrasound (LIPUS) stimulated the viability, proliferation and differentiation of hematopoietic stem/progenitor cells (HSPC) from fresh and cryopreserved peripheral blood leukapheresis product, as well as cord blood when applied for 10 min each day for 4 days. Cell viability, proliferation and differentiation were assessed on day 5 by viable cell counting, MTS proliferation assay, flow cytometry, and colony-forming unit assay. LIPUS stimulation: (i) enhanced the proliferation of fresh HSPC and maintained the viability of cryopreserved HSPC in vitro; (ii) did not affect the percentage of CD34+ and CD14+ cells; and (iii) enhanced burst-forming unit-erythroid colony formation. Hence, we suggest that this novel LIPUS stimulation approach might enhance the efficacy of clinical transplantation and cellular therapies using HSPC.

[1]  Jie Chen,et al.  Ultrasound-enhanced monoclonal antibody production. , 2012, Ultrasound in medicine & biology.

[2]  Sadahiro Iwabuchi,et al.  Low-intensity pulsed ultrasound stimulates cell proliferation and proteoglycan production in rabbit intervertebral disc cells cultured in alginate. , 2006, Biomaterials.

[3]  K. Kaushansky.  Thrombopoietin and Hematopoietic Stem Cell Development , 1999, Annals of the New York Academy of Sciences.

[4]  I. Bernstein,et al.  Ex vivo expansion of human hematopoietic stem and progenitor cells. , 2011, Blood.

[5]  Tarek El-Bialy,et al.  Repair of orthodontically induced root resorption by ultrasound in humans. , 2004, American journal of orthodontics and dentofacial orthopedics : official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics.

[6]  Ming-Dou Ker,et al.  Level shifters for high-speed 1 V to 3.3 V interfaces in a 0.13 /spl mu/m Cu-interconnection/low-k CMOS technology , 2001, 2001 International Symposium on VLSI Technology, Systems, and Applications. Proceedings of Technical Papers (Cat. No.01TH8517).

[7]  R F Kilcoyne,et al.  Acceleration of tibial fracture-healing by non-invasive, low-intensity pulsed ultrasound. , 1994, The Journal of bone and joint surgery. American volume.

[8]  Woon Tiong Ang,et al.  Design and Implementation of Therapeutic Ultrasound Generating Circuit for Dental Tissue Formation and Tooth-Root Healing , 2010, IEEE Transactions on Biomedical Circuits and Systems.

[9]  T. El-Bialy.  Therapeutic ultrasound applications in craniofacial growth, healing and tissue engineering. , 2007, Rejuvenation research.

[10]  C. Rubin,et al.  The use of low-intensity ultrasound to accelerate the healing of fractures. , 2001, The Journal of bone and joint surgery. American volume.

[11]  V. Praloran,et al.  Blood erythroid progenitors (CFU-E and BFU-E) in acute lymphoblastic leukemias , 1989, Blut.

[12]  B. Blanco,et al.  In leukapheresis products from non-Hodgkin's lymphoma patients, the immature hematopoietic progenitors show higher CD90 and CD34 antigenic expression. , 2007, Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis.

[13]  E. Gluckman.  Milestones in umbilical cord blood transplantation. , 2011, Blood reviews.

[14]  Dong Soon Lee,et al.  Introducing pulsed low-intensity ultrasound to culturing human umbilical cord-derived mesenchymal stem cells , 2009, Biotechnology Letters.

[15]  Yajun Ha,et al.  Ultra Storage-Efficient Time Digitizer for Pseudorandom Single Photon Counter Implemented on a Field-Programmable Gate Array , 2010, IEEE Transactions on Biomedical Circuits and Systems.

[16]  S Meghji,et al.  In vitro effects of therapeutic ultrasound on cell proliferation, protein synthesis, and cytokine production by human fibroblasts, osteoblasts, and monocytes. , 1999, Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons.

[17]  Edward A Copelan,et al.  Hematopoietic stem-cell transplantation. , 2006, The New England journal of medicine.

[18]  J M Piret,et al.  Differential cytokine effects on primitive (CD34+CD38-) human hematopoietic cells: novel responses to Flt3-ligand and thrombopoietin , 1996, The Journal of experimental medicine.

[19]  Daeha Lee,et al.  A 100 V, 10 mA high-voltage driver ICs for field emission display applications , 1999, AP-ASIC'99. First IEEE Asia Pacific Conference on ASICs (Cat. No.99EX360).

[20]  S. Iwabuchi,et al.  Low-intensity pulsed ultrasound stimulates cell proliferation, proteoglycan synthesis and expression of growth factor-related genes in human nucleus pulposus cell line. , 2009, European cells & materials.

[21]  E. Vellenga,et al.  Characterization of the erythropoiesis in myelodysplasia by means of ferrokinetic studies, in vitro erythroid colony formation and soluble transferrin receptor , 1998, Leukemia.

[22]  H. Gul-Uludağ,et al.  Cationic liposome-mediated CXCR4 gene delivery into hematopoietic stem/progenitor cells: implications for clinical transplantation and gene therapy. , 2012, Stem cells and development.

[23]  M. Cairo,et al.  Cord blood immunology and stem cell transplantation. , 2005, Human immunology.

[24]  C. Eaves,et al.  Expansion in vitro of transplantable human cord blood stem cells demonstrated using a quantitative assay of their lympho-myeloid repopulating activity in nonobese diabetic-scid/scid mice. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[25]  K. Kaushansky.  Thrombopoietin and the hematopoietic stem cell. , 1998, Blood.

[26]  Byung Hyune Choi,et al.  Low-intensity ultrasound increased colony forming unit-fibroblasts of mesenchymal stem cells during primary culture. , 2011, Tissue engineering. Part C, Methods.

[27]  C. Bordignon.  Stem-cell therapies for blood diseases , 2006, Nature.

[28]  Jinshun Zhao,et al.  Molecular Mechanisms of Low Intensity Pulsed Ultrasound in Human Skin Fibroblasts* , 2004, Journal of Biological Chemistry.

[29]  J. Lo,et al.  Valproic acid increases CXCR4 expression in hematopoietic stem/progenitor cells by chromatin remodeling. , 2009, Stem cells and development.

[30]  J. Dipersio,et al.  Improving stem cell mobilization strategies: future directions , 2009, Bone Marrow Transplantation.