Water self-diffusion behavior in yeast cells studied by pulsed field gradient NMR.

The water self-diffusion behavior in yeast cell water suspension was investigated by pulsed field gradient NMR techniques. Three types of water were detected, which differ according to the self-diffusion coefficients: bulk water, extracellular and intracellular water. Intracellular and extracellular water self-diffusion was restricted; the sizes of restriction regions were approximately 3 and 15-20 microm, respectively. The smallest restriction size was determined as inner cell size. This size and also cell permeability varied with the growth phase of yeast cell. Cell size increased, but permeability decreased with increasing growth time. The values of cell permeabilities P(1)(d) obtained from time dependence of water self-diffusion coefficient were in good agreement with the permeabilities obtained from the exchange rate constants P(1)(eff). The values of P(1)(eff) were 7 x 10(-6), 1.2 x 10(-6) and 1.6 x 10(-6) m/s, and P(1)(d) were 6.3 x 10(-6), 8.4 x 10(-7), 1.5 x 10(-6) m/s for yeast cells incubated for 9 h (exponential growth phase), 24 h (end of exponential growth phase), and 48 h (stationary growth phase), respectively.

[1]  J. E. Tanner,et al.  Restricted Self‐Diffusion of Protons in Colloidal Systems by the Pulsed‐Gradient, Spin‐Echo Method , 1968 .

[2]  P. Kuchel,et al.  Comparative Cell Shape and Diffusional Water Permeability of Red Blood Cells from Indian Elephant (Elephas maximus) and Man (Homo sapiens) , 2000, Comparative Haematology International.

[3]  Schwartz,et al.  Short-time behavior of the diffusion coefficient as a geometrical probe of porous media. , 1993, Physical review. B, Condensed matter.

[4]  A. Anisimov,et al.  Water diffusion in biological porous systems: a NMR approach. , 1998, Magnetic resonance imaging.

[5]  Schwartz,et al.  Diffusion propagator as a probe of the structure of porous media. , 1992, Physical review letters.

[6]  P. Kuchel,et al.  NMR diffusion measurements to characterise membrane transport and solute binding , 1997 .

[7]  A. Sinskey,et al.  Process characteristics of cell lysis mutants of Saccharomyces cerevisisae , 1979, Biotechnology and bioengineering.

[8]  P. Agre,et al.  Functional analyses of aquaporin water channel proteins. , 1999, Methods in enzymology.

[9]  E. Talla,et al.  Identification and functional analysis of the Saccharomyces cerevisiae nicotinamidase gene, PNC1 , 2002, Yeast.

[10]  J. Waugh,et al.  Advances In Magnetic Resonance , 1974 .

[11]  Self-diffusion of water and oil in peanuts investigated by PFG NMR. , 1998, Magnetic resonance imaging.

[12]  P. V. van Zijl,et al.  Functional Analysis of Aquaporin-1 Deficient Red Cells , 1996, The Journal of Biological Chemistry.

[13]  R. Valiullin,et al.  Time dependent self-diffusion coefficient of molecules in porous media , 2001 .

[14]  Jörg Kärger,et al.  Principles and Application of Self-Diffusion Measurements by Nuclear Magnetic Resonance , 1988 .

[15]  R. Valiullin,et al.  Molecular exchange processes in partially filled porous glass as seen with NMR diffusometry , 1997 .

[16]  R. Krämer,et al.  Pulsed high-field gradient in vivo NMR spectroscopy to measure diffusional water permeability in Corynebacterium glutamicum. , 2000, Analytical biochemistry.

[17]  E. Steudle,et al.  Reversible closing of water channels in Chara internodes provides evidence for a composite transport model of the plasma membrane , 1995 .

[18]  Chung-Young Lee,et al.  Leakage of cellular materials from Saccharomyces cerevisiae by ohmic heating , 2002 .

[19]  Qunhui Guo,et al.  Self-diffusion of water-ethanol mixtures in polyacrylic acid-polysulfone composite membranes obtained by pulsed-field gradient nuclear magnetic resonance spectroscopy , 1995 .

[20]  P W Kuchel,et al.  Effects of cholesterol on transmembrane water diffusion in human erythrocytes measured using pulsed field gradient NMR. , 1995, Biophysical chemistry.