that is selectively expressed in B-lymphoid cells and B-CLL The PANE1 gene encodes a novel human minor histocompatibility antigen

Abstract Minor histocompatibility antigens (mHAg) are peptides encoded by polymorphic genes that are presented by MHC molecules and recognized by T cells in recipients of allogeneic hematopoietic cell transplants. Here we report that an alternative transcript of the proliferation associated nuclear element 1 ( PANE1 ) gene encodes a novel HLA-A*0301-restricted mHAg that is selectively expressed in B-lymphoid cells. The antigenic peptide is entirely encoded within a unique exon not present in other PANE1 transcripts. Sequencing of PANE1 alleles in mHAg- positive and mHAg- negative cells demonstrates that differential T cell recognition is due to a single nucleotide polymorphism within the variant exon that replaces an arginine codon with a translation termination codon. The PANE1 transcript that encodes the mHAg is expressed at high levels in resting CD19 + B cells and B-lineage chronic lymphocytic leukemia (B-CLL) cells, and at significantly lower levels in activated B cells. Activation of B-CLL cells through CD40L stimulation decreases expression of the mHAg-encoding

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