Investigation of the effect of taurine on sciatic nerve ischemia-reperfusion injury in rats

Aim: The aim of this study was to investigate the effect of taurine on IR related changes in rat sciatic nerve tissue. Materials-Methods: A total of 32 Spraque Dawley male rats weighing between 250 and 300 grams were equally and randomly divided into four groups as follows: Controls, sham, I/R and taurine+I/R. Taurine was intraperitoneally administered at 200 mg/kg dose prior to the ischemia period in taurine+I/R group. Sciatic nerve tissues were bilaterally excised following the IR process. Tissue malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) levels were measured. Sciatic nerve tissues were histopathologically and immunohistochemically (terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate nick end-labeling, TUNEL; inducible nitric oxide synthase, iNOS; and proliferating cell nuclear antigen PCNA) stained and examined. Results: Histopathological findings have indicated that taurine decreased the sciatic nerve damage and apoptosis. Morever, taurine has also decreased MDA and NO levels and the number of inducible nitric oxide synthase (iNOS) positive cells, but significantly increased tissue SOD, GPx and CAT enzymatic activity levels when compared with the I/R group. Conclusion: Established data from the current study suggest that taurine treatment at 200 mg/kg dose has protective effects against I/R-mdiated sciatic nerve injury.

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