Inhibition of cell proliferation of human gliomas by benzodiazepines in vitro

ABSTRACT— The effects of several benzodiazepines (diazepam, clonazepam, Ro 15–1788 and Ro 5–4864) on cell proliferation of 2 human gliomas were estimated in vitro by means of [3H]‐thymidine uptake assay. It was found that all tested benzodiazepines suppressed [3H]‐thymidine incorporation into the DNA of glioma cells, the effects being stronger in case of peripheral‐type benzodiazepine receptor ligands. The results indicated that benzodiazepines might exert an antiproliferative action on glioma tumour cells growth.

[1]  G. Clarke,et al.  Tranquillizers can block mitogenesis in 3T3 cells and induce differentiation in Friend cells , 1980, Nature.

[2]  M. Pawlikowski,et al.  Diazepam inhibits proliferation of the mouse spleen lymphocytes in vitro. , 1986, Polish journal of pharmacology and pharmacy.

[3]  L. Hertz,et al.  Dissimilarities between benzodiazepine‐binding sites and adenosine uptake sites in astrocytes and neurons in primary cultures , 1987, Journal of neuroscience research.

[4]  E. Zimmerman,et al.  Benzodiazepines have high-affinity binding sites and induce melanogenesis in B16/C3 melanoma cells. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[5]  Á. Kenessey,et al.  Interaction of calcium channel blockers with non-neuronal benzodiazepine binding sites. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[6]  R. E. Hemphill.  Tranquillizers , 1957, The Medical journal of the South-West.

[7]  S. Mizel,et al.  Interleukin 1, a potential regulator of fibroblast proliferation. , 1982, Journal of immunology.

[8]  C. Braestrup,et al.  Specific benzodiazepine receptors in rat brain characterized by high-affinity (3H)diazepam binding. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[9]  E. Kedar,et al.  A rapid technique for isolation of viable tumor cells from solid tumors: use of the tumor cells for induction and measurement of cell-mediated cytotoxic responses. , 1982, European journal of cancer & clinical oncology.

[10]  C. Braestrup,et al.  Benzodiazepine receptors. , 1985, Clinical neuropharmacology.

[11]  J. Tallman,et al.  Benzodiazepine and β-adrenergic receptor ligands independently stimulate phospholipid methylation , 1979, Nature.

[12]  H. Stępień.  LHRH inhibits [3H]thymidine incorporation by pituitary cells cultured in vitro. , 1981, Endocrinology.

[13]  M. Pawlikowski,et al.  Effects of diazepam on cell proliferation in cerebral cortex, anterior pituitary and thymus of developing rats. , 1987, Life sciences.

[14]  C. W. Adams,et al.  Handbook of Neurochemistry , 1983, Springer US.

[15]  John P. Moore,et al.  A common sequence of calcium and pH signals in the mitogenic stimulation of eukaryotic cells , 1985, Nature.

[16]  J. Morgan,et al.  Benzodiazepines that bind at peripheral sites inhibit cell proliferation. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[17]  H. Schoemaker,et al.  Specific high-affinity saturable binding of [3H] R05-4864 to benzodiazepine binding sites in the rat cerebral cortex. , 1981, European journal of pharmacology.