Effects of Salt Concentration and pH on the Absorption Spectra of Polynucleotides with Various S-Values
暂无分享,去创建一个
Poly (A) took stable double-stranded form in 0.02 m phosphate buffer pH 5.8 when its S-value was higher than 3.5. Poly (C) also took stable double-stranded form in 0.01 m acetate buffer pH 3.9 containing 0.1 m NaCl if its S-value was higher than 4.8.Poly (I) with S-values higher than 6.5 stably formed triple-stranded form. Poly (A) with S-values over 3S and poly (I) with S-values over 7S were observed to aggregate in the presence of high concentration of salt, but poly (C) and poly (U) did not aggregate under the same condition. Effect of salt concentration differed with different polynucleotides and with different salts.
[1] B. Janik. Physicochemical Characteristics of Oligonucleotides and Polynucleotides , 1971, Springer US.