Synthesis of Pt Hollow Nanodendrites with Enhanced Peroxidase‐Like Activity against Bacterial Infections: Implication for Wound Healing

Improving the antibacterial activity of H2O2 and reducing its usage are requirements for wound disinfection. Nanomaterials with intrinsic peroxidase‐like properties are developed to enhance the antibacterial performance of H2O2 and avoid the toxicity seen with high H2O2 levels. Here, Pd–Pt core–frame nanodendrites consist of a dense array of platinum (Pt) branches on a Pd core are synthesized, and subsequently converted to Pt hollow nanodendrites by selective removal of the Pd cores by wet etching. The fabricated Pt hollow nanodendrites exert striking peroxidase‐like activity due to the maximized utilization efficiency of the Pt atoms and the presence of high‐index facets on their surfaces. By catalyzing the decomposition of H2O2 into more toxic hydroxyl radicals (•OH), Pt hollow nanodendrites exhibit excellent bactericidal activity against both Gram‐negative and Gram‐positive bacteria with the assistance of low concentrations of H2O2. Furthermore, Pt hollow nanodendrites accelerate wound healing in the presence of low doses of H2O2. In addition, no obvious adverse effects are observed at the given dose of nanodendrites. These findings can be used to guide the design of noble metal‐based nanomaterials as potential enzyme‐mimetic systems and advance the development of nanoenzymes to potentiate the antibacterial activity of H2O2.

[1]  Min Zhou,et al.  Boosting the Peroxidase-Like Activity of Nanostructured Nickel by Inducing Its 3+ Oxidation State in LaNiO3 Perovskite and Its Application for Biomedical Assays , 2017, Theranostics.

[2]  X. Qu,et al.  An Efficient and Benign Antimicrobial Depot Based on Silver-Infused MoS2. , 2017, ACS nano.

[3]  Ge Fang,et al.  Facet Energy versus Enzyme-like Activities: The Unexpected Protection of Palladium Nanocrystals against Oxidative Damage. , 2016, ACS nano.

[4]  Younan Xia,et al.  Platinum Cubic Nanoframes with Enhanced Catalytic Activity and Durability Toward Oxygen Reduction. , 2016, ChemSusChem.

[5]  H. Gu,et al.  Antibiotic-loaded, silver core-embedded mesoporous silica nanovehicles as a synergistic antibacterial agent for the treatment of drug-resistant infections. , 2016, Biomaterials.

[6]  Younan Xia,et al.  Pt-Based Icosahedral Nanocages: Using a Combination of {111} Facets, Twin Defects, and Ultrathin Walls to Greatly Enhance Their Activity toward Oxygen Reduction. , 2016, Nano letters.

[7]  Moon J. Kim,et al.  Pd-Ir Core-Shell Nanocubes: A Type of Highly Efficient and Versatile Peroxidase Mimic. , 2015, ACS nano.

[8]  M. Chi,et al.  Platinum-based nanocages with subnanometer-thick walls and well-defined, controllable facets , 2015, Science.

[9]  X. Qu,et al.  Bifunctionalized Mesoporous Silica‐Supported Gold Nanoparticles: Intrinsic Oxidase and Peroxidase Catalytic Activities for Antibacterial Applications , 2015, Advanced materials.

[10]  Cuiling Li,et al.  Mesoporous Pt hollow cubes with controlled shell thicknesses and investigation of their electrocatalytic performance. , 2014, Chemical communications.

[11]  Yong Hu,et al.  X-ray CT and pneumonia inhibition properties of gold-silver nanoparticles for targeting MRSA induced pneumonia. , 2014, Biomaterials.

[12]  Xiaogang Qu,et al.  Graphene quantum dots-band-aids used for wound disinfection. , 2014, ACS nano.

[13]  J. Callahan,et al.  Photogenerated charge carriers and reactive oxygen species in ZnO/Au hybrid nanostructures with enhanced photocatalytic and antibacterial activity. , 2014, Journal of the American Chemical Society.

[14]  Y. Yamauchi,et al.  All-metal mesoporous nanocolloids: solution-phase synthesis of core-shell Pd@Pt nanoparticles with a designed concave surface. , 2013, Angewandte Chemie.

[15]  Bao Yu Xia,et al.  Highly concave platinum nanoframes with high-index facets and enhanced electrocatalytic properties. , 2013, Angewandte Chemie.

[16]  Y. Yamauchi,et al.  Metallic nanocages: synthesis of bimetallic Pt-Pd hollow nanoparticles with dendritic shells by selective chemical etching. , 2013, Journal of the American Chemical Society.

[17]  Michael R Hamblin,et al.  Antimicrobial strategies centered around reactive oxygen species--bactericidal antibiotics, photodynamic therapy, and beyond. , 2013, FEMS microbiology reviews.

[18]  Jiye Shi,et al.  Graphene Oxide‐Based Antibacterial Cotton Fabrics , 2013, Advanced healthcare materials.

[19]  Haiping Fang,et al.  Destructive extraction of phospholipids from Escherichia coli membranes by graphene nanosheets. , 2013, Nature nanotechnology.

[20]  Lixin Xia,et al.  Templated high-yield synthesis of Pt nanorods enclosed by high-index {311} facets for methanol selective oxidation , 2013 .

[21]  Martin Wasser,et al.  Effects of Hydrogen Peroxide on Wound Healing in Mice in Relation to Oxidative Damage , 2012, PloS one.

[22]  Morteza Mahmoudi,et al.  Antibacterial properties of nanoparticles. , 2012, Trends in biotechnology.

[23]  W. Tremel,et al.  Vanadium pentoxide nanoparticles mimic vanadium haloperoxidases and thwart biofilm formation. , 2012, Nature nanotechnology.

[24]  Y. Niwano,et al.  In vitro and in vivo anti-Staphylococcus aureus activities of a new disinfection system utilizing photolysis of hydrogen peroxide. , 2012, Journal of bioscience and bioengineering.

[25]  Hui Zhang,et al.  Noble-metal nanocrystals with concave surfaces: synthesis and applications. , 2012, Angewandte Chemie.

[26]  Shaoming Huang,et al.  Selective etching induces selective growth and controlled formation of various platinum nanostructures by modifying seed surface free energy. , 2012, ACS nano.

[27]  M. Mahmoudi,et al.  Silver-coated engineered magnetic nanoparticles are promising for the success in the fight against antibacterial resistance threat. , 2012, ACS nano.

[28]  Jing Kong,et al.  Antibacterial activity of graphite, graphite oxide, graphene oxide, and reduced graphene oxide: membrane and oxidative stress. , 2011, ACS nano.

[29]  Younan Xia,et al.  Synthesis of Pd nanocrystals enclosed by {100} facets and with sizes <10 nm for application in CO oxidation , 2011 .

[30]  Omid Akhavan,et al.  Toxicity of graphene and graphene oxide nanowalls against bacteria. , 2010, ACS nano.

[31]  Menachem Elimelech,et al.  Electronic-structure-dependent bacterial cytotoxicity of single-walled carbon nanotubes. , 2010, ACS nano.

[32]  Pedro J. J. Alvarez,et al.  Nanomaterials in the construction industry: a review of their applications and environmental health and safety considerations. , 2010, ACS nano.

[33]  Chunhai Fan,et al.  Graphene-based antibacterial paper. , 2010, ACS nano.

[34]  Jianbo Wu,et al.  Electrochemical synthesis and catalytic property of sub-10 nm platinum cubic nanoboxes. , 2010, Nano letters.

[35]  Christopher T. Walsh,et al.  Antibiotics for Emerging Pathogens , 2009, Science.

[36]  Younan Xia,et al.  Pd-Pt Bimetallic Nanodendrites with High Activity for Oxygen Reduction , 2009, Science.

[37]  Younan Xia,et al.  Shape-controlled synthesis of metal nanocrystals: simple chemistry meets complex physics? , 2009, Angewandte Chemie.

[38]  G. Somorjai,et al.  Localized Pd overgrowth on cubic Pt nanocrystals for enhanced electrocatalytic oxidation of formic acid. , 2008, Journal of the American Chemical Society.

[39]  Zhong Lin Wang,et al.  Synthesis of Tetrahexahedral Platinum Nanocrystals with High-Index Facets and High Electro-Oxidation Activity , 2007, Science.

[40]  Liberato Manna,et al.  Synthesis, properties and perspectives of hybrid nanocrystal structures. , 2006, Chemical Society reviews.

[41]  P. Greenwel,et al.  Role of hydrogen peroxide and oxidative stress in healing responses , 2002, Cellular and Molecular Life Sciences CMLS.

[42]  A. Fujishima,et al.  Detection of active oxidative species in TiO2 photocatalysis using the fluorescence technique , 2000 .

[43]  G. Schackert,et al.  An Easy and Safe Method to Store and Disinfect Explanted Skull Bone , 1999, Acta Neurochirurgica.