In situ hybridization assay for the diagnosis of chagas myocarditis and orchitis in a rhesus macaque (Macaca mulatta): A case report

We present the first documented case of Trypanosoma cruzi‐induced orchitis in a rhesus macaque. Additionally, we describe an in situ hybridization–based assay to confirm T. cruzi infection in formalin‐fixed tissues.

[1]  M. Díaz-Menéndez,et al.  Evidence for Previously Unidentified Sexual Transmission of Protozoan Parasites , 2018, Emerging infectious diseases.

[2]  A. Teixeira,et al.  Sexual transmission of American trypanosomiasis in humans: a new potential pandemic route for Chagas parasites , 2017, Memorias do Instituto Oswaldo Cruz.

[3]  R. Shade,et al.  Correlation between presence of Trypanosoma cruzi DNA in heart tissue of baboons and cynomolgus monkeys, and lymphocytic myocarditis. , 2014, The American journal of tropical medicine and hygiene.

[4]  P. Dorn,et al.  Low prevalence of Chagas parasite infection in a nonhuman primate colony in Louisiana. , 2012, Journal of the American Association for Laboratory Animal Science : JAALAS.

[5]  J. Flanagan,et al.  RNAscope: a novel in situ RNA analysis platform for formalin-fixed, paraffin-embedded tissues. , 2012, The Journal of molecular diagnostics : JMD.

[6]  D. A. Gomes,et al.  Inheritance of DNA Transferred from American Trypanosomes to Human Hosts , 2010, PloS one.

[7]  R. Guardado-Mendoza,et al.  Nonspecific lymphocytic myocarditis in baboons is associated with Trypanosoma cruzi infection. , 2009, The American journal of tropical medicine and hygiene.

[8]  G. Hubbard,et al.  Natural Chagas disease in four baboons , 2009, Journal of medical primatology.

[9]  G. Hubbard,et al.  Fatal acute Chagas disease in a chimpanzee , 2009, Journal of medical primatology.

[10]  G. Hubbard,et al.  Trypanosoma cruzi in non‐human primates with a history of stillbirths: a retrospective study (Papio hamadryas spp.) and case report (Macaca fascicularis) , 2008, Journal of medical primatology.

[11]  Carlos Chagas Tripanosomiase americana: forma aguda da molestia , 1916 .

[12]  Gaspar Vianna Contribuição para o estudo da anatomia patolojica da "Molestia de Carlos Chagas": (Esquizotripanoze humana ou tireoidite parazitaria) , 1911 .