Cytotoxicity versus antibacterial activity of some antiseptics in vitro.

Through the development of new techniques for bacterial growth and isolation, obligate anaerobes have been shown to be more prevalent than previously thought in the pathogenesis of periapical and pulpal diseases. A follow-up study was conducted to examine the germicidal effect of 0.05% NaOCl, 10 mg/ml metronidazole and supernatant of Ca (OH)2 (0.025 ml) on four anaerobic microorganisms commonly found in root canals, and to compare the toxicity of these substances on cell cultures. In vitro testing revealed that 0.05% NaOCl and Ca (OH)2 were both equally effective on these anaerobes. Also, metronidazole was found to be germicidally effective against Bacteroides melaninogenicus, Bacteroides oralis and Peptostreptococcus anaerobius, but ineffective against Veillonella alcalescens. Furthermore, it was found that NaOCl and Ca (OH)2 had a very destructive effect on cell cultures compared with their antimicrobial effect, whereas metronidazole was less toxic among the agents tested.

[1]  S. Montgomery,et al.  Effectiveness of ultrasonics and calcium hydroxide for the debridement of human mandibular molars. , 1989, Journal of endodontics.

[2]  M. Iwaku,et al.  Bactericidal efficacy of metronidazole against bacteria of human carious dentin in vivo. , 1989, Caries research.

[3]  M. Cvek,et al.  Effects of calcium hydroxide and sodium hypochlorite on the dissolution of necrotic porcine muscle tissue. , 1988, Journal of endodontics.

[4]  M. Iwaku,et al.  Bactericidal efficacy of metronidazole against bacteria of human carious dentin in vitro. , 1988, Caries research.

[5]  D. Mitchell.  Metronidazole: its use in clinical dentistry. , 1984, Journal of clinical periodontology.

[6]  L. Grossman,et al.  Evaluation of the antimicrobial potential of calcium hydroxide as an intracanal medicament. , 1983, Journal of endodontics.

[7]  F. Carranza,et al.  The effect of metronidazole on human periodontal disease. A clinical and bacteriological study. , 1983, Journal of periodontology.

[8]  C. Torneck,et al.  The effect of calcium hydroxide on porcine pulp fibroblasts in vitro. , 1983, Journal of endodontics.

[9]  G Sundqvist,et al.  Bacteriologic evaluation of the effect of 0.5 percent sodium hypochlorite in endodontic therapy. , 1983, Oral surgery, oral medicine, and oral pathology.

[10]  W. J. Loesche,et al.  Treatment of periodontal infections due to anaerobic bacteria with short-term treatment with metronidazole. , 1981, Journal of clinical periodontology.

[11]  L. Hollender,et al.  Treatment of non-vital permanent incisors with calcium hydroxide. VI. A clinical, microbiological and radiological evaluation of treatment in one sitting of teeth with mature or immature root. , 1976, Odontologisk revy.

[12]  L. Spångberg,et al.  Biologic effects of dental materials. 3. Toxicity and antimicrobial effect of endodontic antiseptics in vitro. , 1973, Oral surgery, oral medicine, and oral pathology.