High-throughput screening of enzyme libraries: in vitro evolution of a beta-galactosidase by fluorescence-activated sorting of double emulsions.
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[1] Dan S. Tawfik,et al. High-throughput screening of enzyme libraries: thiolactonases evolved by fluorescence-activated sorting of single cells in emulsion compartments. , 2005, Chemistry & biology.
[2] George Georgiou,et al. Engineering of protease variants exhibiting high catalytic activity and exquisite substrate selectivity. , 2005, Proceedings of the National Academy of Sciences of the United States of America.
[3] Andrew D Griffiths,et al. High-throughput screens and selections of enzyme-encoding genes. , 2005, Current opinion in chemical biology.
[4] Patrick S Daugherty,et al. Evolutionary optimization of fluorescent proteins for intracellular FRET , 2005, Nature Biotechnology.
[5] Dan S. Tawfik,et al. Directed evolution of protein inhibitors of DNA-nucleases by in vitro compartmentalization (IVC) and nano-droplet delivery. , 2005, Journal of molecular biology.
[6] D. Neri,et al. Covalent DNA display as a novel tool for directed evolution of proteins in vitro. , 2004, Protein engineering, design & selection : PEDS.
[7] Susanne Wilhelm,et al. Ultra-high-throughput screening based on cell-surface display and fluorescence-activated cell sorting for the identification of novel biocatalysts. , 2004, Current opinion in biotechnology.
[8] George Georgiou,et al. Anchored periplasmic expression, a versatile technology for the isolation of high-affinity antibodies from Escherichia coli-expressed libraries. , 2004, Proceedings of the National Academy of Sciences of the United States of America.
[9] R. Woodgate,et al. Generic expansion of the substrate spectrum of a DNA polymerase by directed evolution , 2004, Nature Biotechnology.
[10] Dan S. Tawfik,et al. In vitro compartmentalization by double emulsions: sorting and gene enrichment by fluorescence activated cell sorting. , 2004, Analytical biochemistry.
[11] Stephen J Benkovic,et al. Enhanced crossover SCRATCHY: construction and high-throughput screening of a combinatorial library containing multiple non-homologous crossovers. , 2003, Nucleic acids research.
[12] Hiroshi Yanagawa,et al. DNA display for in vitro selection of diverse peptide libraries. , 2003, Nucleic acids research.
[13] B. Hall. The EBG System of E. coli: Origin and Evolution of a Novel β-Galactosidase for the Metabolism of Lactose , 2003, Genetica.
[14] H. Steven Wiley,et al. Flow-cytometric isolation of human antibodies from a nonimmune Saccharomyces cerevisiae surface display library , 2003, Nature Biotechnology.
[15] Dan S. Tawfik,et al. Directed evolution of an extremely fast phosphotriesterase by in vitro compartmentalization , 2003, The EMBO journal.
[16] Dan S. Tawfik,et al. Microbead display by in vitro compartmentalisation: selection for binding using flow cytometry , 2002, FEBS letters.
[17] Virginia W Cornish,et al. Milestones in directed enzyme evolution. , 2002, Current opinion in chemical biology.
[18] Dan S. Tawfik,et al. Investigating the target recognition of DNA cytosine-5 methyltransferase HhaI by library selection using in vitro compartmentalisation. , 2002, Nucleic acids research.
[19] A. Christmann,et al. Display of Passenger Proteins on the Surface ofEscherichia coli K-12 by the Enterohemorrhagic E. coli Intimin EaeA , 2001, Journal of bacteriology.
[20] G. Georgiou,et al. High-throughput antibody isolation. , 2001, Current opinion in chemical biology.
[21] B. Matthews,et al. A structural view of the action of Escherichia coli (lacZ) beta-galactosidase. , 2001, Biochemistry.
[22] Takuya Ueda,et al. Cell-free translation reconstituted with purified components , 2001, Nature Biotechnology.
[23] K D Wittrup,et al. Protein engineering by cell-surface display. , 2001, Current opinion in biotechnology.
[24] G. Georgiou,et al. Isolation of high-affinity ligand-binding proteins by periplasmic expression with cytometric screening (PECS) , 2001, Nature Biotechnology.
[25] Jennifer L. Ong,et al. Directed evolution of polymerase function by compartmentalized self-replication , 2001, Proceedings of the National Academy of Sciences of the United States of America.
[26] Mark J. Olsen,et al. Function-based isolation of novel enzymes from a large library , 2000, Nature Biotechnology.
[27] Dan S. Tawfik,et al. Man-made enzymes--from design to in vitro compartmentalisation. , 2000, Current opinion in biotechnology.
[28] Michele C. Kieke,et al. Directed evolution of a stable scaffold for T-cell receptor engineering , 2000, Nature Biotechnology.
[29] Stephen J Benkovic,et al. Using an AraC-based three-hybrid system to detect biocatalysts in vivo , 2000, Nature Biotechnology.
[30] N. Doi,et al. STABLE: protein‐DNA fusion system for screening of combinatorial protein libraries in vitro , 1999, FEBS letters.
[31] B. Hall. Experimental evolution of Ebg enzyme provides clues about the evolution of catalysis and to evolutionary potential. , 1999, FEMS microbiology letters.
[32] B. Hall,et al. Determining the evolutionary potential of a gene. , 1998, Molecular biology and evolution.
[33] Dan S. Tawfik,et al. Man-made cell-like compartments for molecular evolution , 1998, Nature Biotechnology.
[34] D. M. Brown,et al. An approach to random mutagenesis of DNA using mixtures of triphosphate derivatives of nucleoside analogues. , 1996, Journal of molecular biology.
[35] B. Hall,et al. Catalysis by the large subunit of the second beta-galactosidase of Escherichia coli in the absence of the small subunit. , 1995, The Biochemical journal.
[36] M. Molina,et al. Use of fluorescein-di-beta-D-galactopyranoside (FDG) and C12-FDG as substrates for beta-galactosidase detection by flow cytometry in animal, bacterial, and yeast cells , 1994, Applied and environmental microbiology.
[37] W. Stemmer. Rapid evolution of a protein in vitro by DNA shuffling , 1994, Nature.
[38] D. Kompala,et al. A single‐cell assay of β‐galactosidase in recombinant Escherichia coli using flow cytometry , 1993 .
[39] Zhijian Huang,et al. Kinetic fluorescence measurement of fluorescein di-beta-D-galactoside hydrolysis by beta-galactosidase: intermediate channeling in stepwise catalysis by a free single enzyme. , 1991, Biochemistry.
[40] R. Lamed,et al. Flow cytometry sorting of viable bacteria and yeasts according to beta-galactosidase activity , 1990, Applied and environmental microbiology.
[41] J. Bailey,et al. A single‐cell assay of β‐galactosidase activity in Saccharomyces cerevisiae , 1988 .
[42] G P Nolan,et al. Fluorescence-activated cell analysis and sorting of viable mammalian cells based on beta-D-galactosidase activity after transduction of Escherichia coli lacZ. , 1988, Proceedings of the National Academy of Sciences of the United States of America.
[43] R. Macdonald,et al. A fluorescence assay to monitor vesicle fusion and lysis. , 1982, The Journal of biological chemistry.
[44] B. Hall,et al. Evolution of a new enzymatic function by recombination within a gene. , 1980, Proceedings of the National Academy of Sciences of the United States of America.
[45] J. Lengyel,et al. Evolution of a Second Gene for β-Galactosidase in Escherichia coli , 1973 .
[46] R. Warren. Lactose-utilizing mutants of lac deletion strains of Escherichia coli. , 1972, Canadian journal of microbiology.
[47] Dan S. Tawfik,et al. Altering the sequence specificity of HaeIII methyltransferase by directed evolution using in vitro compartmentalization. , 2004, Protein engineering, design & selection : PEDS.
[48] N. Doi,et al. In vitro selection of restriction endonucleases by in vitro compartmentalization. , 2004, Nucleic acids research.
[49] G. Georgiou. Analysis of large libraries of protein mutants using flow cytometry. , 2000, Advances in protein chemistry.
[50] G. Nolan,et al. Improved FACS-Gal: flow cytometric analysis and sorting of viable eukaryotic cells expressing reporter gene constructs. , 1991, Cytometry.