Effects of dextran on the self-association of human spectrin.

The self-association of human spectrin is enhanced in the presence of dextran. The equilibrium constant for association of two heterodimers to form a tetramer is increased by an order of magnitude in the presence of 20% dextran. The rate constant for association is also enhanced, while the rate constant for dissociation is almost independent of dextran concentration. The degree of enhancement of association is dependent only on the mass concentration of dextran; for a given mass concentration of dextran the effect is independent of dextran molecular weight. These effects are believed to be due to excluded volume phenomena, and a model is presented that realistically accounts for the effects. These results imply that the association of spectrin within the erythrocyte will be enhanced by the presence of hemoglobin.

[1]  Jan Hermans,et al.  Excluded‐volume theory of polymer–protein interactions based on polymer chain statistics , 1982 .

[2]  Ralph G. Pearson,et al.  Kinetics and mechanism , 1961 .

[3]  A. Minton.  Excluded volume as a determinant of macromolecular structure and reactivity , 1981 .

[4]  G. Ralston.  Temperature and pH dependence of the self-association of human spectrin. , 1991, Biochemistry.

[5]  L. D. Ward,et al.  Effects of thermodynamic nonideality in kinetic studies. , 1983, Archives of biochemistry and biophysics.

[6]  R. Traut,et al.  Quantification of Coomassie Blue stained proteins in polyacrylamide gels based on analyses of eluted dye. , 1975, Analytical biochemistry.

[7]  D. Speicher,et al.  The complete cDNA and polypeptide sequences of human erythroid alpha-spectrin. , 1990, The Journal of biological chemistry.

[8]  S. Zimmerman,et al.  Effects of macromolecular crowding on the association of E. coli ribosomal particles. , 1988, Nucleic acids research.

[9]  J. Dunbar,et al.  Hydrodynamic characterization of the heterodimer of spectrin. , 1981, Biochimica et biophysica acta.

[10]  E. Ungewickell,et al.  Self-association of human spectrin. A thermodynamic and kinetic study. , 1978, European journal of biochemistry.

[11]  V. Bennett,et al.  The spectrin-actin junction of erythrocyte membrane skeletons. , 1989, Biochimica et biophysica acta.

[12]  G. Ralston,et al.  Physico-chemical characterization of the spectrin tetramer from bovine erythrocyte membranes. , 1976, Biochimica et biophysica acta.

[13]  D. Winzor,et al.  Thermodynamic nonideality in macromolecular solutions. Evaluation of parameters for the prediction of covolume effects. , 1990, European journal of biochemistry.

[14]  R. Josephs,et al.  Structural study of spectrin from human erythrocyte membranes. , 1977, Biochemistry.

[15]  A. Ogston,et al.  Effect of inert polymers on protein self‐association , 1981, FEBS letters.

[16]  D. Wallach,et al.  Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. , 1971, Biochemistry.

[17]  P. V. von Hippel,et al.  "Macromolecular crowding": thermodynamic consequences for protein-protein interactions within the T4 DNA replication complex. , 1990, The Journal of biological chemistry.

[18]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[19]  V. Marchesi,et al.  Full-length sequence of the cDNA for human erythroid beta-spectrin. , 1990, The Journal of biological chemistry.

[20]  A. Ogston,et al.  An approach to the study of phase separation in ternary aqueous systems. , 1968, The Biochemical journal.

[21]  S. Zimmerman,et al.  Macromolecular crowding extends the range of conditions under which DNA polymerase is functional. , 1988, Biochimica et biophysica acta.

[22]  A. Minton,et al.  Analysis of non-ideal behavior in concentrated hemoglobin solutions. , 1977, Journal of molecular biology.

[23]  T. Laurent.  THE INTERACTION BETWEEN POLYSACCHARIDES AND OTHER MACROMOLECULES. 5. THE SOLUBILITY OF PROTEINS IN THE PRESENCE OF DEXTRAN. , 1963, The Biochemical journal.

[24]  S. Liu,et al.  Hemoglobin enhances the self-association of spectrin heterodimers in human erythrocytes. , 1984, The Journal of biological chemistry.

[25]  Vann Bennett,et al.  Partial deficiency of erythrocyte spectrin in hereditary spherocytosis , 1985, Nature.